Osteoarthritis(OA)progresses due to the excessive generation of reactive oxygen and nitrogen species(ROS/RNS)and abnormal ATP energy metabolism related to the oxidative phosphorylation pathway in the mitochondria.High...Osteoarthritis(OA)progresses due to the excessive generation of reactive oxygen and nitrogen species(ROS/RNS)and abnormal ATP energy metabolism related to the oxidative phosphorylation pathway in the mitochondria.Highly active single-atom nanozymes(SAzymes)can help regulate the redox balance and have shown their potential in the treatment of inflammatory diseases.In this study,we innovatively utilised ligand-mediated strategies to chelate Pt^(4+)with modified g-C_(3)N_(4)byπ-πinteraction to prepare g-C_(3)N_(4)-loaded Pt single-atom(Pt SA/C_(3)N_(4))nanozymes that serve as superoxide dismutase(SOD)/catalase(CAT)mimics to scavenge ROS/RNS and regulate mitochondrial ATP production,ultimately delaying the progression of OA.Pt SA/C_(3)N_(4)exhibited a high loading of Pt single atoms(2.45 wt%),with an excellent photothermal conversion efficiency(54.71%),resulting in tunable catalytic activities under near-infrared light(NIR)irradiation.Interestingly,the Pt-N_(6) active centres in Pt SA/C_(3)N_(4)formed electron capture sites for electron holes,in which g-C_(3)N_(4)regulated the d-band centre of Pt,and the N-rich sites transferred electrons to Pt,leading to the enhanced adsorption of free radicals and thus higher SOD-and CAT-like activities compared with pure g-C_(3)N_(4)and g-C_(3)N_(4)-loaded Pt nanoparticles(Pt NPs/C_(3)N_(4)).Based on the use of H_(2)O_(2)-induced chondrocytes to simulate ROS-injured cartilage in vitro and an OA joint model in vivo,the results showed that Pt SA/C_(3)N_(4)could reduce oxidative stress-induced damage,protect mitochondrial function,inhibit inflammation progression,and rebuild the OA microenvironment,thereby delaying the progression of OA.In particular,under NIR light irradiation,Pt SA/C_(3)N_(4)could help reverse the oxidative stress-induced joint cartilage damage,bringing it closer to the state of the normal cartilage.Mechanistically,Pt SA/C_(3)N_(4)regulated the expression of mitochondrial respiratory chain complexes,mainly NDUFV2 of complex 1 and MT-ATP6 of ATP synthase,to reduce ROS/RNS and promote ATP production.This study provides novel insights into the design of artificial nanozymes for treating oxidative stress-induced inflammatory diseases.展开更多
We regret that an error was made in“A novel inhibitor of MDM2 oncogene blocks metastasis of hepatocellular carcinoma and overcomes chemoresistance”(Genes Dis 2019 Dec;694:419e430).In Fig.1C of the original manuscrip...We regret that an error was made in“A novel inhibitor of MDM2 oncogene blocks metastasis of hepatocellular carcinoma and overcomes chemoresistance”(Genes Dis 2019 Dec;694:419e430).In Fig.1C of the original manuscript,the right panel(showing the migration ability of the MHCCLM3 cells)was erroneously duplicated with the left panel(showing the migration ability of the Huh7 cells)when the figures were assembled.We have now checked the original data and request to replace the right panel with the correct data for MHCCLM3 cells.The corrected Fig.展开更多
基金the support from the Guangxi Natural Science Foundation(No.2023GXNSFBA026020)Guangxi Scientific Research and Technological Development Foundation(Grant No.GuikeAB21220062)National Natural Science Foundation of China(Grant No.82160429,82360426,52301303,81960400).
文摘Osteoarthritis(OA)progresses due to the excessive generation of reactive oxygen and nitrogen species(ROS/RNS)and abnormal ATP energy metabolism related to the oxidative phosphorylation pathway in the mitochondria.Highly active single-atom nanozymes(SAzymes)can help regulate the redox balance and have shown their potential in the treatment of inflammatory diseases.In this study,we innovatively utilised ligand-mediated strategies to chelate Pt^(4+)with modified g-C_(3)N_(4)byπ-πinteraction to prepare g-C_(3)N_(4)-loaded Pt single-atom(Pt SA/C_(3)N_(4))nanozymes that serve as superoxide dismutase(SOD)/catalase(CAT)mimics to scavenge ROS/RNS and regulate mitochondrial ATP production,ultimately delaying the progression of OA.Pt SA/C_(3)N_(4)exhibited a high loading of Pt single atoms(2.45 wt%),with an excellent photothermal conversion efficiency(54.71%),resulting in tunable catalytic activities under near-infrared light(NIR)irradiation.Interestingly,the Pt-N_(6) active centres in Pt SA/C_(3)N_(4)formed electron capture sites for electron holes,in which g-C_(3)N_(4)regulated the d-band centre of Pt,and the N-rich sites transferred electrons to Pt,leading to the enhanced adsorption of free radicals and thus higher SOD-and CAT-like activities compared with pure g-C_(3)N_(4)and g-C_(3)N_(4)-loaded Pt nanoparticles(Pt NPs/C_(3)N_(4)).Based on the use of H_(2)O_(2)-induced chondrocytes to simulate ROS-injured cartilage in vitro and an OA joint model in vivo,the results showed that Pt SA/C_(3)N_(4)could reduce oxidative stress-induced damage,protect mitochondrial function,inhibit inflammation progression,and rebuild the OA microenvironment,thereby delaying the progression of OA.In particular,under NIR light irradiation,Pt SA/C_(3)N_(4)could help reverse the oxidative stress-induced joint cartilage damage,bringing it closer to the state of the normal cartilage.Mechanistically,Pt SA/C_(3)N_(4)regulated the expression of mitochondrial respiratory chain complexes,mainly NDUFV2 of complex 1 and MT-ATP6 of ATP synthase,to reduce ROS/RNS and promote ATP production.This study provides novel insights into the design of artificial nanozymes for treating oxidative stress-induced inflammatory diseases.
文摘We regret that an error was made in“A novel inhibitor of MDM2 oncogene blocks metastasis of hepatocellular carcinoma and overcomes chemoresistance”(Genes Dis 2019 Dec;694:419e430).In Fig.1C of the original manuscript,the right panel(showing the migration ability of the MHCCLM3 cells)was erroneously duplicated with the left panel(showing the migration ability of the Huh7 cells)when the figures were assembled.We have now checked the original data and request to replace the right panel with the correct data for MHCCLM3 cells.The corrected Fig.
