Hexagonal-phase NaYF_(4)(β-NaYF_(4))has been acknowledged to be one of the most efficient doping hosts to prepare bright lanthanide-doped luminescent nano-bioprobes for various biomedical applications.However,to date...Hexagonal-phase NaYF_(4)(β-NaYF_(4))has been acknowledged to be one of the most efficient doping hosts to prepare bright lanthanide-doped luminescent nano-bioprobes for various biomedical applications.However,to date,it remains a great challenge to synthesize ultra-bright lanthanide-dopedβ-NaYF_(4)nano-bioprobes under a low reaction temperature by using conventional synthetic methods.Herein,we first develop an acetic acid(HAc)-mediated coprecipitation method for the preparation of ultrabright lanthanide-dopedβ-NaYF_(4)nanoprobes under a low reaction temperature at 200℃.Based on a series of comparative spectroscopic investigations,we show that the use of HAc in the reaction environment can not only promote the rapidα-βphase transformation of NaYF_(4)host at 200℃ within 1 h but also boost the absolute photoluminescence quantum yield(PLQY)of NaYF_(4)nanocrystals to 30.68%for near-infrared emission and to 3.79%for upconversion luminescence,both of which are amongst the highest values for diverse lanthanide-doped luminescent nanocrystals ever reported.By virtue of their superior nearinfrared luminescence,we achieve optical-guided dynamic vasculature imaging in vivo of the whole body at a high spatial resolution(23.8μm)under 980 nm excitation,indicating its potential for the diagnosis and treatment evaluation of vasculaturerelated diseases.展开更多
Multiplexed intracellular detection is desirable in biomedical sciences for its higher eficiency and accuracy compared to the single-analyte detection.However,it is very challenging to construct nanoprobes that posses...Multiplexed intracellular detection is desirable in biomedical sciences for its higher eficiency and accuracy compared to the single-analyte detection.However,it is very challenging to construct nanoprobes that possess multiple fluorescent signals to recognize the different intracellular species synchronously.Herein,we proposed a novel dual-excitation/dual-emission upconversion strategy for multiplexed detection through the design of upconversion nanoparticles(UCNP)loaded with two dyes for sensitization and quenching of the upconversion luminescence(UCL),respectively.Based on the two independent energy transfer processes of near-infrared(NIR)dye IR845 to UCNP and UCNP to visible dye PAPS-Zn,CIO-and Zn2+were simultaneously detected with a limit of detection(LOD)of41.4 and 10.5 nM,respectively.By tilizing a purpose built 830/980 nm dual-laser confocal microscope,both intrinsic and exogenous CIO and Zn2+in live MCF-7 cells have been accurately quantified.Such dual-excitation/dual-emission ratiometric UCL detection mode enables not only monitoring multiple intracellular analytes but also eliminating the detection deviation caused by inhomogeneous probe distribution in cells.Through modulation of NIR dye and visible dye with other reactive groups,the nanoprobes can be extended to analyze various intraellular species,which provides a promising tool to study the biological activities in live cells and diagnose diseases.展开更多
Near-infrared(NIR)light,which has ignorable tissue scattering/absorption,minimal photodamage,and no autofluorescence interference,is highly favorable for bioapplications.NIR dye and lanthanide-doped nanoparticle(LnNP)...Near-infrared(NIR)light,which has ignorable tissue scattering/absorption,minimal photodamage,and no autofluorescence interference,is highly favorable for bioapplications.NIR dye and lanthanide-doped nanoparticle(LnNP),as representative NIR-excited luminescence probes,have attracted increasing interest due to their unique optical property and low biological toxicity.Design of luminescence probes based on NIR dye/LnNP nanocomposites cannot only integrate the advantages but also achieve additional functions via regulating internal energy transfer pathways.In this review,we focus on the most recent advances in the development of NIR dye/LnNP nanocomposites as potential bioprobes,which cover from their fundamental photophysics to bioapplications,including energy transfer mechanisms,interface engineering(involving binding interaction,distance,and aggregation as key factors),and their applications for dye-sensitized upconversion/downshifting luminescent bioimaging,detection of biomolecules,and NIR-triggered diagnosis and therapy.Some future prospects and efforts toward this active research field are also envisioned.展开更多
基金This work was supported by the Fund of Fujian Science&Technology Innovation Laboratory for Optoelectronic Information(No.2020ZZ114)the Key Research Program of Frontier Science CAS(No.QYZDY-SSW-SLH025)+2 种基金the National Natural Science Foundation of China(Nos.21871256 and 12204481)the Natural Science Foundation of Fujian Province(No.2022J01211422)Fund of Advanced Energy Science and Technology Guangdong Laboratory(No.DJLTN0200/DJLTN0240).
文摘Hexagonal-phase NaYF_(4)(β-NaYF_(4))has been acknowledged to be one of the most efficient doping hosts to prepare bright lanthanide-doped luminescent nano-bioprobes for various biomedical applications.However,to date,it remains a great challenge to synthesize ultra-bright lanthanide-dopedβ-NaYF_(4)nano-bioprobes under a low reaction temperature by using conventional synthetic methods.Herein,we first develop an acetic acid(HAc)-mediated coprecipitation method for the preparation of ultrabright lanthanide-dopedβ-NaYF_(4)nanoprobes under a low reaction temperature at 200℃.Based on a series of comparative spectroscopic investigations,we show that the use of HAc in the reaction environment can not only promote the rapidα-βphase transformation of NaYF_(4)host at 200℃ within 1 h but also boost the absolute photoluminescence quantum yield(PLQY)of NaYF_(4)nanocrystals to 30.68%for near-infrared emission and to 3.79%for upconversion luminescence,both of which are amongst the highest values for diverse lanthanide-doped luminescent nanocrystals ever reported.By virtue of their superior nearinfrared luminescence,we achieve optical-guided dynamic vasculature imaging in vivo of the whole body at a high spatial resolution(23.8μm)under 980 nm excitation,indicating its potential for the diagnosis and treatment evaluation of vasculaturerelated diseases.
基金the Science and Technology Cooperation Fund between Chinese and Australian Governments(No.2017YFE0132300)the Strategic Priority Research Program of the CAS(No.XDB20000000)+2 种基金the National Natural Science Foundation of China(Nos.51672272,21771185,21771178,and 21975257)Youth Innovation Promotion Association of CAS(No.2017347)the CAS/SAFEA International Partnership Program for Creative Research Teams.
文摘Multiplexed intracellular detection is desirable in biomedical sciences for its higher eficiency and accuracy compared to the single-analyte detection.However,it is very challenging to construct nanoprobes that possess multiple fluorescent signals to recognize the different intracellular species synchronously.Herein,we proposed a novel dual-excitation/dual-emission upconversion strategy for multiplexed detection through the design of upconversion nanoparticles(UCNP)loaded with two dyes for sensitization and quenching of the upconversion luminescence(UCL),respectively.Based on the two independent energy transfer processes of near-infrared(NIR)dye IR845 to UCNP and UCNP to visible dye PAPS-Zn,CIO-and Zn2+were simultaneously detected with a limit of detection(LOD)of41.4 and 10.5 nM,respectively.By tilizing a purpose built 830/980 nm dual-laser confocal microscope,both intrinsic and exogenous CIO and Zn2+in live MCF-7 cells have been accurately quantified.Such dual-excitation/dual-emission ratiometric UCL detection mode enables not only monitoring multiple intracellular analytes but also eliminating the detection deviation caused by inhomogeneous probe distribution in cells.Through modulation of NIR dye and visible dye with other reactive groups,the nanoprobes can be extended to analyze various intraellular species,which provides a promising tool to study the biological activities in live cells and diagnose diseases.
基金supported by the Fund of Fujian Science&Technology Innovation Laboratory for Optoelectronic Information(2020ZZ114)the Key Research Program of Frontier Science CAS(QYZDY-SSW-SLH025)+1 种基金the National Natural Science Foundation of China(21731006 and 21871256)the Fund of Advanced Energy Science and Technology Guangdong Laboratory(DJLTN0200/DJLTN0240)。
基金Science andTechnologyCooperation Fund between Chinese and AustralianGovernments,Grant/Award Number:2017YFE0132300Strategic Priority Research Program of the CAS,Grant/Award Number:XDB20000000+1 种基金NSFC,Grant/Award Numbers:51672272,21771185,21771178,21975257,12074380Youth Innovation Promotion Association of CAS,Grant/Award Number:2017347。
文摘Near-infrared(NIR)light,which has ignorable tissue scattering/absorption,minimal photodamage,and no autofluorescence interference,is highly favorable for bioapplications.NIR dye and lanthanide-doped nanoparticle(LnNP),as representative NIR-excited luminescence probes,have attracted increasing interest due to their unique optical property and low biological toxicity.Design of luminescence probes based on NIR dye/LnNP nanocomposites cannot only integrate the advantages but also achieve additional functions via regulating internal energy transfer pathways.In this review,we focus on the most recent advances in the development of NIR dye/LnNP nanocomposites as potential bioprobes,which cover from their fundamental photophysics to bioapplications,including energy transfer mechanisms,interface engineering(involving binding interaction,distance,and aggregation as key factors),and their applications for dye-sensitized upconversion/downshifting luminescent bioimaging,detection of biomolecules,and NIR-triggered diagnosis and therapy.Some future prospects and efforts toward this active research field are also envisioned.
