The competition between Bidens pilosa and Setaria viridis alters soil microbial composition and soil ecological function

Bidens pilosa is recognized as one of the major invasive plants in China.Its invasion has been associated with significant losses in agriculture,forestry,husbandry,and biodiversity.Soil ecosystems play an important role in alien plant invasion.Microorganisms within the soil act as intermediaries between plants and soil ecological functions,playing a role in regulating soil enzyme activities and nutrient dynamics.Understanding the interactions between invasive plants,soil microorganisms,and soil ecological processes is vital for managing and mitigating the impacts of invasive species on the environment.In this study,we conducted a systematic analysis focusing on B.pilosa and Setaria viridis,a common native companion plant in the invaded area.To simulate the invasion process of B.pilosa,we constructed homogeneous plots consisting of B.pilosa and S.viridis grown separately as monocultures,as well as in mixtures.The rhizosphere and bulk soils were collected from the alien plant B.pilosa and the native plant S.viridis.In order to focus on the soil ecological functional mechanisms that contribute to the successful invasion of B.pilosa,we analyzed the effects of B.pilosa on the composition of soil microbial communities and soil ecological functions.The results showed that the biomass of B.pilosa increased by 27.51% and that of S.viridis was significantly reduced by 66.56%.The organic matter contents in the bulk and rhizosphere soils of B.pilosa were approximately 1.30 times those in the native plant soils.The TN and NO_(3)^(-)contents in the rhizosphere soil of B.pilosa were 1.30 to 2.71 times those in the native plant soils.The activities of acid phosphatase,alkaline phosphatase,and urease in the rhizosphere soil of B.pilosa were 1.98-2.25 times higher than in the native plant soils.Using high-throughput sequencing of the16S rRNA gene,we found that B.pilosa altered the composition of the soil microbial community.Specifically,many genera in Actinobacteria and Proteobacteria were enriched in B.pilosa soils.Further correlation analyses verified that these genera had significantly positive relationships with soil nutrients and enzyme activities.Plant biomass,soil p H,and the contents of organic matter,TN,NO_(3)^(-),TP,AP,TK,and AK were the main factors affecting soil microbial communities.This study showed that the invasion of B.pilosa led to significant alterations in the composition of the soil microbial communities.These changes were closely linked to modifications in plant traits as well as soil physical and chemical properties.Some microbial species related to C,N and P cycling were enriched in the soil invaded by B.pilosa.These findings provide additional support for the hypothesis of soil-microbe feedback in the successful invasion of alien plants.They also offer insights into the ecological mechanism by which soil microbes contribute to the successful invasion of B.pilosa.Overall,our research contributes to a better understanding of the complex interactions between invasive plants,soil microbial communities,and ecosystem dynamics.

Effects of shrub-grass patterns on soil detachment and hydraulic parameters of slope in the Pisha sandstone area of Inner Mongolia,China

The characteristics of soil holding capacity for different shrub-grass patterns are important to research the mechanisms regulating vegetation on slopes.The objective of this study was to describe the characteristics and mecha-nisms of soil erosion and hydraulic parameters under differ-ent vegetation patterns in the Pisha sandstone area of Inner Mongolia on lands of 8°slope gradient.We carried out field scouring experiments on five different shrub-grass patterns as treatments,viz no shrubs(GL),shrubs on the upper part of the slope(SU),middle part of the slope(SM)and lower part of the slope(SL).We designated bare slope(BL)as the control.We employed three different water flow rates(15,20,30 L·min^(−1)).Our results showed that the contribution of plant root systems to slope sediment reduction ranged from 64 to 84%.The root systems proved to be the main contributing factor to reduction of erosion by vegetation.The relationship between soil detachment rate,stream flow power,and flow unit stream power under different scouring discharge rates showed that soil detachment declined in rank order as:BL>GL>SU>SM>SL.The SL pat-tern had the lowest soil detachment rate(0.098 g·m^(−2)·s^(−1)),flow stream power(2.371 W·m^(−2)),flow unit stream power(0.165 m·s^(−1))and flow shear stress(16.986 Pa),and proved to be the best erosion combating pattern.The results of decision coefficient and path analysis showed that stream power was the most important hydraulic parameter for describing soil detachment rate.The combination of stream power and shear stress,namely Dr=0.1ω−0.03τ−0.56(R^(2)=0.924),most accurately simulated the soil detachment characteristics on slopes.Our study suggests that the risk of soil ero-sion can be reduced by planting shrub-grass mixes on these slopes.Under the conditions of limited water resources and economy,the benefit of sediment reduction can be maxi-mized by planting shrubbery on the lower parts of slopes.

Study on Environmental Multi-parameter Sensor Based on MEMS

A new membrane type Al_2O_3 micromachining material is used.We develop an environmental multi-parameter detection micro-system,which implements the detection to temperature,humidity,wind speed,and CO.The test results illustrate that the heat-release unit in micro-system intercross greatly affects other sensing units on the temperature.We study the method of etching process,which formed cavity to reduce the heat exchange efficiency and decrease temperature intercross effect.

Integrated Gas Sensor Based on MEMS

Integrated gas sensor suspending bridge style micro-structure has been developed on Al_2O_3 new type membrane based on MEMS.CH_4 gas sensing unit and CO gas sensing unit were fabricated separately on 4 unit array style suspending bridge structure.We have studied on time-sequence modulation form voltage pulse heating method which forms different work temperature

四川盆地中侏罗统沙溪庙组天然气地球化学特征及成因

四川盆地中侏罗统沙溪庙组是低油价形势下四川盆地天然气勘探的重要领域,但盆地内不同地区天然气来源尚不明确,影响下一步勘探部署决策,为此开展侏罗系沙溪庙组天然气地球化学特征及成因研究。结果表明:(1)沙溪庙组天然气属于干酪根降解气,甲烷含量>84%,含少量乙烷、丙烷等烃类气体及少量的氮气、二氧化碳等非烃气体,不含硫化氢,不同区域的天然气成熟度存在差别;(2)天然气δ^(13)C_(1)值为-39.2‰~-31.2‰、δ^(13)C_(2)值为-32.8‰~-22.3‰、δ^(13)C3值为-28.7‰~-19.5‰,天然气碳同位素未发生倒转,川西地区为煤成气,川中地区为油型气,川东地区为煤成气和油型气混合气,以油型气为主;(3)不同区域天然气δ^(13)C_(1)值、δ^(13)C_(2)值的差异,与其来源于不同类型烃源岩贡献比例大小有关。川西、川西南地区主力烃源岩为须五段煤系烃源岩,川中地区为下侏罗统湖相烃源岩,川东地区天然气来源于须五段和下侏罗统烃源岩。研究结果对四川盆地侏罗系沙溪庙组下一步天然气勘探部署决策具有重要的指导意义。

Andrographolide and Its Derivative Potassium Dehydrographolide Succinate Suppress PRRSV Replication in Primary and Established Cells via Differential Mechanisms of Action

Porcine reproductive and respiratory syndrome virus(PRRSV) continues to cause significant economic loss worldwide and remains a serious threat to the pork industry. Currently, vaccination strategies provide limited protection against PRRSV infection, and consequently, new antiviral strategies are urgently required. Andrographolide(Andro) and its derivative potassium dehydrographolide succinate(PDS) have been used clinically in China and other Asian countries as therapies for inflammation-related diseases, including bacterial and viral infections, for decades. Here, we demonstrate that Andro and PDS exhibit robust activity against PRRSV replication in Marc-145 cells and primary porcine alveolar macrophages(PAMs). The two compounds exhibited broad-spectrum inhibitory activities in vitro against clinically circulating type 2 PRRSV GD-HD, XH-GD, and NADC30-like HNhx strains in China. The EC_(50)values of Andro against three tested PRRSV strain infections in Marc-145 cells ranged from 11.7 to 15.3 lmol/L, with selectivity indexes ranging from 8.3 to10.8, while the EC_(50)values of PDS ranged from 57.1 to 85.4 lmol/L, with selectivity indexes ranging from 344 to 515.Mechanistically, the anti-PRRSV activity of the two compounds is closely associated with their potent suppression on NFj B activation and enhanced oxidative stress induced by PRRSV infection. Further mechanistic investigations revealed that PDS, but not Andro, is able to directly interact with PRRSV particles. Taken together, our findings suggest that Andro and PDS are promising PRRSV inhibitors in vitro and deserves further in vivo studies in swine.

Maternal heterozygous mutation in CHEK1 leads to mitotic arrest in human zygotes

Dear Editor,The first mitotic division in zygotes is crucial for the beginning of the life cycle for the human.After fertilization,zygotes reactivate cell cycle,both paternal and maternal genomes replicate and reprogram to become totipotent.In the meantime,the male and female pronucleus move to the center of the zygote and merge.Then zygotes enter the metaphase,and sister chromatids separate into two daughter cells(Eckersley-Maslin et al.,2018;Reichmann et al.,2018).This is a sensitive time window and many perturbances may cause the first mitosis to fail.

中国氦气资源成藏规律与开发前景

氦气是一种关系国民经济和高新技术产业发展的全球性稀缺资源,中国工业用氦主要依赖进口,因此急需明确氦气成藏富集规律、寻找富氦气田,落实中国氦气资源家底和开发潜力。系统梳理了全球氦气资源分布及开发现状,开展国内氦气资源普查与分布研究,剖析了国内典型富氦气田气藏特征及成藏条件,明确了中国主要富氦气田氦气成因来源和富集成藏主控因素,认为中国天然气中氦气主要以壳源为主,指出相对浅埋的富铀钍古老花岗岩基底或侵入体、早期形成的大型稳定古隆起或古潜山、良好的上覆巨厚膏盐岩或泥岩盖层、沟通基底和储层的运移通道是氦气成藏富集的主控因素,建立了富氦常规气、富氦页岩气、富氦非烃气和富氦水溶气4种不同类型富氦气藏的成藏模式并预测富氦有利勘探区。在此基础上分析了中国氦气资源开发前景,提出了寻找富氦气田,加强中、低丰度氦气资源综合开发利用是未来提高中国国内氦气产量的重要方式。