LncFASA promotes cancer ferroptosis via modulating PRDX1 phase separation

Ferroptosis, a unique type of non-apoptotic cell death resulting from iron-dependent lipid peroxidation, has a potential physiological function in tumor suppression, but its underlying mechanisms have not been fully elucidated. Here, we report that the long non-coding RNA(lncRNA) LncFASA increases the susceptibility of triple-negative breast cancer(TNBC) to ferroptosis. As a tumor suppressor, LncFASA drives the formation of droplets containing peroxiredoxin1(PRDX1), a member of the peroxidase family, resulting in the accumulation of lipid peroxidation via the SLC7A11-GPX4 axis. Mechanistically, LncFASA directly binds to the Ahpc-TSA domain of PRDX1, inhibiting its peroxidase activity by driving liquid-liquid phase separation, which disrupts intracellular ROS homeostasis. Notably, high LncFASA expression indicates favorable overall survival in individuals with breast cancer, and LncFASA impairs the growth of breast xenograft tumors by modulating ferroptosis. Together, our findings illustrate the crucial role of this lncRNA in ferroptosis-mediated cancer development and provide new insights into therapeutic strategies for breast cancer.

Dispersion of“guava-like”silica/polyacrylate nanocomposite particles in polyacrylate matrix

A series of“guava-like”silica/polyacrylate nanocomposite particles with close silica content and different grafting degrees were prepared via mini-emulsion polymerization using 3-(trimethoxysilyl)propyl methacrylate(TSPM)modified silica/acrylate dispersion.The silica/polyacrylate composite particles were melt-mixed with unfilled polyacrylate(PA)resin to prepare corresponding silica/polyacrylate molded composites and the dispersion mechanism of these silica particles from the“guava-like”composite particles into polyacrylate matrix was studied.It was calculated that about 110 silica particles were accumulated in the bulk of every silica/polyacrylate composite latex particle.Both the solubility tests of silica/polyacrylate composite latex particles in tetrahydrofuran(THF)and the section transmission electron microscope(TEM)micrographs of silica/polyacrylate molded composites indicated that the grafting degree of silica particles played a crucial role in the dispersion of silica/polyacrylate composite particles into the polyacrylate matrix.When the grafting degree of polyacrylate onto silica was in a moderate range(ca.20%-70%),almost all of silica particles in these“guava-like”composite particles were dispersed into the polyacrylate matrix in a primaryparticle-level.However,at a lower grafting degree,massive silica aggregations were found in molded composites because of the lack of steric protection.At a greater grafting degree(i.e.,200%),a cross-linked network was formed in the silica/polyacrylate composite particles,which prevented the dispersion of composite particles in THF and polyacrylate matrix as primary particles.

Phenolic rigid organic filler/isotactic polypropylene composites. Ⅱ. Tensile properties

A novel phenolic rigid organic filler(KT)was used to modify isotactic polypropylene(iPP).The influence of KT particles on the tensile properties of PP/KT micro-composites was studied by uniaxial tensile test and the mor-phological structures of the stretched specimens were observed by scanning electron microscopy(SEM)and polarized optical microscopy(POM).We found that the Young's modulus of PP/KT specimens increased with filler content,while the yield and break of the specimens are related to the filler particles size.The yield stress,the break-ing stress and the ultimate elongation of PP/KT specimens were close to those of unfilled iPP specimens when the max-imal filler particles size is less than a critical value,which is 7μm at a crosshead speed of 10 mm/min and 3μm at 200 mm/min,close to that of glass bead but far more than those of other rigid inorganic filler particles.The interfacial interaction was further estimated from yield stress,indi-cating that KT particles have a moderate interracial inter-action with iPP matrix.Thus,the incorporation of small KT particles can reinforce iPP matrix and simultaneously cause few detrimental effects on the other excellent tensile properties of iPP matrix,due to their organic nature,higher specific area,solid true-spherical shape and the homogen-ous dispersion of the ROF particles in microcomposites.