Differences in the Content of Protein and Essential Amino Acids between Different Rice Varieties

Evaluation of the protein content is a major indicator of the nutritional quality of rice,and the protein quality of rice is the best among cereal crops. Essential amino acids play an irreplaceable role in human growth,development and health care. Essential amino acid is a key ingredient to measure the nutritional value of rice. Using the experimental rice processed from the rice variety " Yuzhenxiang" sprayed with plant nutrients( patent number: ZL201110103910. 9),namely high essential amino acid nutritional rice,combined with five kinds of high quality rice imported by COFCO and homegrown " Wuchang rice",we send the samples of the seven kinds of rice to Hunan Food Testing Center,and adopt HPLC method to test the content of protein and eight kinds of essential amino acids. Three bags of rice are randomly selected for each kind of rice,and each bag is a replication. The test results show that there are highly significant differences in the content of essential amino acids between different kinds of rice( F = 246. 29**,P =5 ×10- 71),and there are also highly significant differences in the content between different kinds of essential amino acids( F = 3937. 09**,P = 4 × 10- 146). The test results of protein content indicate that there are highly significant differences in the content of protein between different kinds of rice( F = 3937. 0973. 29**,P =5. 81 ×10- 11),and the test results of lysine content show that there are highly significant differences in the content of lysine between different kinds of rice( F =3937. 0973. 29**,P =5. 81 ×10- 11).

Early Screening of Rice Varieties with High Essential Amino Acids by Aseptic Seedling Method

With 5 rice varieties( Yifeng 3321,Yifeng 3315,Yifeng 3317,Yifeng 45,Yifeng 14-24) as the experimental objects,the aseptic seedling method was used to culture the rice seeds,and the HPLC was used to determine the content of amino acids in aseptic rice seedlings as well as the total content of 8 kinds of essential amino acids and the content of protein. And the content of protein and amino acids in the polished rice of 5 varieties was detected,and the analysis of variance was performed for screening of the varieties containing high essential amino acids. The rice with high essential amino acids could be produced to meet people's rice quality requirements. The analysis of variance indicated that there were significant differences( F = 39. 262,p = 0. 01) in the content of essential amino acids among 5 kinds of rice varieties. By the Duncan's multiple comparison,the differences among rice varieties were calculated to obtain screening results. The aseptic seedlings:Yifeng 3321(7. 268),Yifeng 3315(6. 395),Yifeng 3317(5. 698),Yifeng 45(5. 567),Yifeng 14-24(5. 190). Rice: Yifeng 3321(2.998),Yifeng 14-24(2. 627),Yifeng 3317(2. 611),Yifeng 3315(2. 503),Yifeng 45(2. 281). Based on the estimated human daily requirement of essential amino acids and amino acids requirement pattern,it was found that the polished rice of 5 varieties was up to standard.The variety with the highest content of essential amino acids was Yifeng 3317(3830 mg/100g),followed by Yifeng 3321(3770 mg/100 g).The results showed that the high quality rice variety in this experiment was Yifeng 3321.

Study on Preparation Technology of Taurine in Rice Peptide Feed

With rice as experimental material,the multi-strain solid-state fermentation method was used to study the preparation technology of taurine,and the analysis of variance was employed to determine the optimal process of taurine preparation as follows: fermentation time(72h); amount of cysteinyl acid( 2. 5 g),amount of methionine( 2. 5 g); ratio of Bacillus subtilis YS-45 to yeast S-78 to Aspergillus niger PL-39( 1 ∶ 2 ∶ 1). Under the optimal process,the taurine content reached 117. 8 mg/g.

Effects of Nano-sustained Release Sodium Butyrate on Growth and Intestinal Cell Proliferation of Grass Carps

Taking grass carps with the initial weight of about 20g as the research object,the basic feeds of grass carps were added with0.0%,0.1%,0.3%,0.6%,0.8%,and 1% of nano-sustained release sodium butyrate to prepare 6 types of experimental feeds with equal nitrogen and energy.The effects of different concentrations of nano-sustained release sodium butyrate were surveyed on growth and intestinal cell proliferation of grass carps.The experiment was carried out in cages with 50 carps per cage,and each treatment was repeated 3 times for60 days.Experimental results indicated that the addition of nano-sustained release sodium butyrate significantly promoted the growth of grass carps and significantly increased the ratio of intestinal villus to crypt depth.When the addition of nano-sustained release sodium butyrate was0.6%,the weight increase rate,specific growth rate,fullness and intestinal villus height of grass carps were the highest,which was significantly higher than that of the control group(P < 0.05).The study results indicated that addition of appropriate amount of nano-sustained release sodium butyrate can promote the growth of grass carps through increasing the intestinal villus height,and the suitable addition dosage was0.6%.