目的:比较国内外新型冠状病毒肺炎(以下简称新冠肺炎)心理健康研究的现状并分析未来研究方向。方法:通过筛选中英文检索词后组成检索式,在中文数据库CNKI和英文数据库Web of Science核心合集中分别检索关于新冠肺炎疫情对心理健康影响...目的:比较国内外新型冠状病毒肺炎(以下简称新冠肺炎)心理健康研究的现状并分析未来研究方向。方法:通过筛选中英文检索词后组成检索式,在中文数据库CNKI和英文数据库Web of Science核心合集中分别检索关于新冠肺炎疫情对心理健康影响的文献,然后基于CiteSpace软件从期刊来源、国家与机构、研究热点等方面对所搜集到的文献进行可视化分析。结果:截止到2020年6月12日,从Web of Science核心合集上搜索到英文文献175篇,从CNKI搜索到中文文献118篇。结论:中国在新冠肺炎疫情心理健康的研究中发挥了积极作用,心理健康研究符合我国国情发展,注重心理干预是未来国内外的发展趋势。展开更多
Plant mitochondrial phosphate transporters regulate phosphate transport and ATP synthesis. Determining whether they function in abiotic stress response process would shed light on their response to salt stress. We use...Plant mitochondrial phosphate transporters regulate phosphate transport and ATP synthesis. Determining whether they function in abiotic stress response process would shed light on their response to salt stress. We used the CRISPR/Cas9 gene-editing system to mutagenize two mitochondrial phosphate transporters, OsMPT3;1 and OsMPT3;2, to investigate their regulatory roles under salt stress. Two cas9(CRISPR-associated protein9)-free homozygous mutants, mpt33 and mpt30, were confirmed to be stable. Both OsMPT3;1 and OsMPT3;2 were markedly induced by salt stress, and their mutagenesis strongly inhibited growth and development, especially under salt stress. Mutagenesis sharply reduced the accumulation of ATP, phosphate, calcium, soluble sugar, and proline and increased osmotic potential, malondialdehyde, and Na^+ /K^+ ratio under salt stress. Both mutants demonstrate normal growth and development in the presence of ATP, revealing high sensitivity to exogenous ATP under salt stress. The mutants showed lowered rates of Na^+ efflux but also of K^+ and Ca^(2+) influx under salt stress. Mutagenesis of OsMPT3;2 altered the enrichment profiles of differentially expressed genes involved mainly in synthesis of secondary metabolites, metabolism of glycolysis, pyruvate, tricarboxylic acid cycle, in response to salt stress. The mutant displayed significant accumulation differences in 14 metabolites involved in 17 metabolic pathways, and strongly up-regulated the accumulation of glutamine, a precursor in proline synthesis, under salt stress. These findings suggest that the OsMPT3 gene modulates phosphate transport and energy supply for ATP synthesis and triggers changes in accumulation of ions and metabolites participating in osmotic regulation in rice under salt stress, thus increasing rice salt tolerance. This study demonstrates the effective application of CRISPR/Cas9 gene-editing to the investigation of plant functional genes.展开更多
在钠离子电池正极材料体系中,钠超离子导体型Na_(3)V_(2)(PO_(4))_(3)(NVP)因其高电压平台和良好的循环稳定性而备受关注,然而,其本征电导率较差严重限制了其实际应用。通过球磨辅助固相法制备了稀土离子(La^(3+),Gd^(3+),Lu^(3+))掺杂...在钠离子电池正极材料体系中,钠超离子导体型Na_(3)V_(2)(PO_(4))_(3)(NVP)因其高电压平台和良好的循环稳定性而备受关注,然而,其本征电导率较差严重限制了其实际应用。通过球磨辅助固相法制备了稀土离子(La^(3+),Gd^(3+),Lu^(3+))掺杂的Na_(3)V_(2-x)RE_(x)(PO_(4))_(3)/C (x=0,0.01,0.03,0.05)正极材料。通过X射线衍射(XRD)、扫描电镜(SEM)、X射线能谱(EDS)证实了稀土离子(La^(3+),Gd^(3+),Lu^(3+))在NVP/C结构中的成功掺杂,同时通过电化学测试研究了稀土离子(La^(3+),Gd^(3+),Lu^(3+))掺杂对NVP/C的电化学性能的影响。结果表明,La^(3+)和Gd^(3+)的掺杂均能在不同程度上改善NVP/C材料的电化学性能。NVP/C-La0.03和NVP/C-Gd0.03在1C倍率下的初始放电比容量分别为110和105.5 m Ah·g^(-1),200次循环后容量保持率分别为97.5%和95.7%。在这些掺杂样品中NVP/C-La0.03表现出的电化学性能最佳,即使在5C的倍率下循环500次后,放电比容量仍可达100.05 m Ah·g^(-1)。因此,适当的稀土离子掺杂对提高NVP/C正极材料的电化学性能有一定的提升作用,为钠离子电池正极材料的深入探索提供了基础研究支撑。展开更多
文摘目的:比较国内外新型冠状病毒肺炎(以下简称新冠肺炎)心理健康研究的现状并分析未来研究方向。方法:通过筛选中英文检索词后组成检索式,在中文数据库CNKI和英文数据库Web of Science核心合集中分别检索关于新冠肺炎疫情对心理健康影响的文献,然后基于CiteSpace软件从期刊来源、国家与机构、研究热点等方面对所搜集到的文献进行可视化分析。结果:截止到2020年6月12日,从Web of Science核心合集上搜索到英文文献175篇,从CNKI搜索到中文文献118篇。结论:中国在新冠肺炎疫情心理健康的研究中发挥了积极作用,心理健康研究符合我国国情发展,注重心理干预是未来国内外的发展趋势。
基金supported by the National Key Research and Development Program of China(2016YFC0501203)the National Genetically Modified Organism Project(2016ZX08010005-9)。
文摘Plant mitochondrial phosphate transporters regulate phosphate transport and ATP synthesis. Determining whether they function in abiotic stress response process would shed light on their response to salt stress. We used the CRISPR/Cas9 gene-editing system to mutagenize two mitochondrial phosphate transporters, OsMPT3;1 and OsMPT3;2, to investigate their regulatory roles under salt stress. Two cas9(CRISPR-associated protein9)-free homozygous mutants, mpt33 and mpt30, were confirmed to be stable. Both OsMPT3;1 and OsMPT3;2 were markedly induced by salt stress, and their mutagenesis strongly inhibited growth and development, especially under salt stress. Mutagenesis sharply reduced the accumulation of ATP, phosphate, calcium, soluble sugar, and proline and increased osmotic potential, malondialdehyde, and Na^+ /K^+ ratio under salt stress. Both mutants demonstrate normal growth and development in the presence of ATP, revealing high sensitivity to exogenous ATP under salt stress. The mutants showed lowered rates of Na^+ efflux but also of K^+ and Ca^(2+) influx under salt stress. Mutagenesis of OsMPT3;2 altered the enrichment profiles of differentially expressed genes involved mainly in synthesis of secondary metabolites, metabolism of glycolysis, pyruvate, tricarboxylic acid cycle, in response to salt stress. The mutant displayed significant accumulation differences in 14 metabolites involved in 17 metabolic pathways, and strongly up-regulated the accumulation of glutamine, a precursor in proline synthesis, under salt stress. These findings suggest that the OsMPT3 gene modulates phosphate transport and energy supply for ATP synthesis and triggers changes in accumulation of ions and metabolites participating in osmotic regulation in rice under salt stress, thus increasing rice salt tolerance. This study demonstrates the effective application of CRISPR/Cas9 gene-editing to the investigation of plant functional genes.
文摘在钠离子电池正极材料体系中,钠超离子导体型Na_(3)V_(2)(PO_(4))_(3)(NVP)因其高电压平台和良好的循环稳定性而备受关注,然而,其本征电导率较差严重限制了其实际应用。通过球磨辅助固相法制备了稀土离子(La^(3+),Gd^(3+),Lu^(3+))掺杂的Na_(3)V_(2-x)RE_(x)(PO_(4))_(3)/C (x=0,0.01,0.03,0.05)正极材料。通过X射线衍射(XRD)、扫描电镜(SEM)、X射线能谱(EDS)证实了稀土离子(La^(3+),Gd^(3+),Lu^(3+))在NVP/C结构中的成功掺杂,同时通过电化学测试研究了稀土离子(La^(3+),Gd^(3+),Lu^(3+))掺杂对NVP/C的电化学性能的影响。结果表明,La^(3+)和Gd^(3+)的掺杂均能在不同程度上改善NVP/C材料的电化学性能。NVP/C-La0.03和NVP/C-Gd0.03在1C倍率下的初始放电比容量分别为110和105.5 m Ah·g^(-1),200次循环后容量保持率分别为97.5%和95.7%。在这些掺杂样品中NVP/C-La0.03表现出的电化学性能最佳,即使在5C的倍率下循环500次后,放电比容量仍可达100.05 m Ah·g^(-1)。因此,适当的稀土离子掺杂对提高NVP/C正极材料的电化学性能有一定的提升作用,为钠离子电池正极材料的深入探索提供了基础研究支撑。
