Seabuckthorn juice alleviates allergic symptoms in shrimp-induced food allergy mice

Tropomyosin(TM)in shrimp is one of the predominant causes of food allergy around the world.In the present study,the effect of seabuckthorn juice against TM-induced shrimp allergy was investigated in BALB/c mice.Allergic symptoms,spleen index,intestinal section and diarrhea were measured in shrimp allergy mice.As the results,seabuckthorn juice suppressed the lesions in jejunum tissue,diarrhea and allergic symptoms in shrimp allergy mice.Seabuckthorn juice also reduced serum concentrations of tumor necrosis factor-a(TNF-α)as well as immunoglobulin E(IgE)and stimulated the secretion of interleukin-10(IL-10)in mice with shrimp allergy.Taken together,our findings suggest that increased IL-10 by seabuckthorn juice inhibits Th2 cytokine production to suppress shrimp allergic symptoms.Furthermore,seabuckthorn juice also regulates shrimp allergy by reducing jejunum lesions,inhibiting levels of TNF-αand IgE.

A hippocampal anti-hypertensive mechanism induced by twirling reinforcing-reducing manipulation in rats

Objective:To investigate a hippocampal anti-hypertensive mechanism induced by twirling reinforcingreducing manipulation(TRRM)using proteomics in rats.Methods:Forty-two male spontaneously hypertensive rats were randomly divided into 3 groups,and 14 Wistar-Kyoto rats were served as control group.In the twirling reinforcing(TRF)group,needles were directly inserted into the Taichong(LR 3)point,then thumbs were moved heavily forward and lightly backward for 3 min,while needles remained inserted for 17 min.In the twirling reduction(TRD)group,the same treatment was applied as in the TRF group except that the thumb moved lightly forward and heavily backward.In the model and control groups,only the corresponding grasping and fixation were applied.All interventions were conducted for 14 days.The blood pressure(BP)of all rats was measured one day before intervention and every other day after.Then hematoxylin-eosin(H&E)staining,label-free and parallel reaction monitoring proteomic techniques were used to assess hippocampal samples from each group.Results:Systolic BPs showed a significant decrease in the TRF and TRD groups compared with the model group(P<.01).In the model group,H&E staining showed obvious pathological changes in the hippocampus,while in the TRF and TRD groups,the hippocampal morphology was only slightly altered.Labelfree proteomic analysis revealed 1163 differential protein expressions between groups.Gene Ontology enrichment analysis confirmed that the differentially expressed proteins were enriched in different biological processes,cellular components,and molecular functions.Kyoto Encyclopedia of Genes and Genomes enrichment analysis showed that TRRM proteins were expressed in the serotonergic synapse pathway,renin-angiotensin system,the mitogen-activated protein kinase signaling pathway,and the peroxisome pathway,which were all also related to BP regulation.Conclusion:TRRM can significantly lower the BP of SHRs.The mode of action may be through the activation of various protein pathways in the hippocampus that are related to BP regulation.

Adenosine cyclic phosphate with ultrasonic-assisted pectinase extraction alleviated allergic reactions in RBL-2H3 through inhibiting the influx of intracellular Ca^(2+)

Jujube contains abundant cyclic adenosine monophosphate(cAMP)and the ultrasonic-assisted pectinase extraction(UAPE)conditions for obtaining the maximum cAMP yield from jujube were optimized.Orthogonal array design was applied to evaluate the effects of 4 variables by UAPE on cAMP yield.The results showed that the optimal cAMP yield(783.0μg/g)was derived at ratio of liquid to solid 5 mL/g,ratio of pectinase to raw material 1.5%,time 60 min and temperature 40℃.Moreover,the effect of cAMP on the anti-allergic function of action induced by immunoglobulin E(IgE)and its meschanism was investigated through establishing the sensitized cell model in rat basophilic leukemia(RBL-2 H3)cells using dinitrophenylated(DNP)-bovine serum albumin(BSA)-IgE.The results showed that cAMP interfered with sensitized cells,effectively inhibited the occurrence of basophil degranulation in dose dependence,and significantly reduced the activity ofβ-hexosamindase(β-hex),at the optimal concentration of 50μg/mL.The level of anti-inflammatory factor interleukin-10(IL-10)was promoted and the content of pro-inflammatory factor tumor necrosis factor-α(TNF-α)was suppressed by cAMP.In addition,influx of intracellular Ca^(2+) was repressed effectively.Our results demonstrate that jujube cAMP regulated the cytokine balance in the allergy pathway through blocking the influx of extracellular Ca^(2+),with the prevention of allergy symptoms.

Accumulative Effect of Soil Organic Carbon and Nitrogen in Water-Stable Aggregates and Soil Stability Characteristics of Robinia pseudoacacia Plantation in the Loess Hilly-Gully Region

Soil water-stable aggregates (WSAs) are the basic unit of soil constitution and can contribute to remaining the stable soil constitution. The objective of this study was to clarify the distribution and stability of WSAs and the soil organic carbon (SOC), the total nitrogen (TN), and the total phosphorus (TP) concentrations in 0 - 20 cm and 20 - 40 cm soil layers under the different ages of Robinia pseudoacacia plantations. The 20, 25, 40, and 50 years-old Robinia pseudoacacia plantations were selected. Stepwise regression analysis showed that >5 mm and 1 - 2 mm WSAs, SOC concentration in 2 - 5 mm WSAs, and TN and TP concentrations in < 0.25 mm WSAs were dominant independent variables affecting aggregate stability and that SOC in 0.25 - 0.5 mm WSAs, TN in <0.25 mm and 1 - 2 mm WSAs and TP in 2 - 5 mm WSAs were dominant independent variables affecting SOC, TN, and TP concentrations in bulk soils.

系统性红斑狼疮心脏受累患者临床特征及免疫抑制干预对并发全心脏扩大者的效果

目的总结心脏受累系统性红斑狼疮(SLE)患者临床特征,分析该类患者临床特点及免疫抑制治疗后的预后。方法回顾性分析2010年1月-2017年1月新疆医科大学第一附属医院风湿免疫科诊治的902例SLE患者临床资料。结果 902例患者中,532例无心脏损害,370例出现心脏损害。370例心脏损害患者中,男性45例,女性325例。男女间比较,心悸、胸痛、胸闷表现差异有统计学意义(P<0.05);心电图方面,T波改变、房室传导阻滞、PR间期延长差异有统计学意义(P<0.05);心脏超声(UCG)中,少量、中量的心包积液差异有统计学意义(P<0.05);有无心脏损害两者间比较,贫血、肾脏病变比例差异有统计学意义(P<0.05)。有心脏损害患者的补体C3、高密度脂蛋白低于无心脏损害者(P<0.05),而起病至免疫抑制剂治疗前的时间大于无心脏损害者(P<0.05)。有无全心扩大两者间比较,贫血、肾脏病变比例差异有统计学意义(P<0.05),全心扩大组贫血、肾脏病变比例高于无全心扩大组。全心扩大组左心室射血分数(LVEF)低于无全心扩大组(P<0.05),病程、起病至免疫抑制剂治疗前的时间、左心室舒张末期内径(LVEDD)大于无全心扩大组(P<0.05)。心脏扩大7例患者UCG指标治疗前后水平差异有统计学意义(P<0.05)。结论 SLE患者心脏损害较为常见,全心脏扩大者早期免疫干预治疗对保护心脏有益。免疫抑制治疗对心脏扩大SLE患者心脏结构及功能的变化有改善。

Experimental detection of bubble-wall interactions in a vertical gas–liquid flow

Bubble motions and bubble-wall interactions in stagnant liquid were experimentally investigated by high-speed CCD and PIV technique with the main feature parameters such as E?tv?s numbers Eo = 0.98–1.10, Morton number Mo = 3.21 × 10^(-9)and Reynolds numbers Re = 180 ~ 190. The effect of bubble injecting frequency and the distance S between the gas injection nozzle and the wall on the statistical trajectory of bubbles, average velocity distribution of flow field and Reynolds shear stress were studied in detail. It was shown that the combination of bubble injecting frequency and the distance S caused different bubble motion forms and hydrodynamic characteristics.When the normalized initial distance was very little, like S*≈ 1.2(here S*= 2S/d_e, and deis the bubble equivalent diameter), bubbles ascended in a zigzag trajectory with alternant structure of high and low speed flow field around the bubbles, and the distribution of positive and negative Reynolds shear stress looked like a blob. With the increase of distance S*, bubbles' trajectory would tend to be smooth and straight from the zigzag curve. Meanwhile, with the increase of bubble injecting frequency, the camber of bubble trajectory at 20<y<60 mm had a slight increase due to the inhibitory effect from the vertical wall. Under larger spacing, such as S*≈ 3.6, the low-frequency bubbles gradually moved away from the vertical plane wall in a straight trajectory and the high-frequency bubbles gradually moved close to the vertical wall in a similar straight trajectory after an unstable camber motion. Under the circumstances, high-speed fluid was mainly distributed in the region between the wall and the bubbles, while the relative large Reynolds shear stress mainly existed in the region far away from the wall.

Investigation and analysis of lower extremity arterial disease in hospitalized elderly type 2 diabetic patients

Background:The risk of lower extremity arterial disease (LEAD) is increased in diabetic patients.LEAD in diabetic patients occurs earlier and is often more severe and diffuse;however,it is largely underdiagnosed and untreated.The purposes of this study were to investigate and analyze LEAD situation of hospitalized elderly type 2 diabetic patients.Methods:The ankle-brachial index (ABI) was used to screen LEAD in hospitalized elderly type 2 diabetic patients.The patients were divided into 5 groups based on the screening results:non-LEAD group and LEAD group;the LEAD group was divided into mild stenosis group,moderate stenosis group,and severe stenosis group.Results:The percentage of patients who had LEAD was 43%.Significant difference in age,diabetes duration,peak velocity,microalbuminuria,and vibratory sensory neuropathy was observed between patients with and without LEAD;regression analysis showed that urinary albumin and vibratory sensory neuropathy were independent risk factors for LEAD.Significant difference in age,body mass index (BMI),peak velocity,urinary albumin,and high-density lipoprotein cholesterol (HDL-C) was observed between mild stenosis group,moderate stenosis group,and severe stenosis group;regression analysis showed that urinary albumin,BMI,and HDL-C were independent risk factors for accelerating vascular stenosis.Conclusions:The incidence of LEAD in hospitalized elderly type 2 diabetic patients is high;age,diabetes duration,peak velocity,BMI,urinary microalbumin,vibratory sensory neuropathy,and HDL-C are the maior risk factors for LEAD.Active control of risk factors is helpful to reduce or delay LEAD.

The characteristics of walking strategy in elderly patients with type 2 diabetes

Objective:To explore the walking strategy by monitoring the characteristics of center of pressure(COP)of gait in the elderly with type2 diabetes.Methods:All of the elderly patients with type2 diabetes(n=543)were enrolled from Huadong Hospital Affiliated to Fudan University.Dynamic barefoot plantar pressure was assessed by Footscan7 USB2 flat.Outcome measures included excursion,the x-and ycoordinates displacement of COP and falling frequency.Results:There were 64.5%of cases with abnormal COP trajectory.Among them,45.2%were with abnormal fold-back,14.0%with two or more abnormal fold-back,20.5%with abnormal beginning point deviating from the heel to the arch and metatarsal region,18.0%with abnormal terminal point deviating from the hallux to toe 2e5 and the x-and y-coordinates displacement of COP in both feet are asymmetry.Conclusions:It highlights to put forward the walking strategy according to the abnormal COP trajectory.Due to the elderly diabetics with high risks of falling,the rehabilitation nursing should be strengthened mainly including the training of enhancing proprioception to prevent the elderly patients with type2 diabetes from falling.

Effect of combining music media therapy with lower extremity exercise on elderly patients with diabetes mellitus

Objective:To evaluate the compliance with lower extremity exercise and blood circulation in the feet of elderly diabetics following a combination of music media therapy and a lower extremity exercise regimen.Method:The 72 elderly diabetic patient subjects were divided into two groups:control group(n=38)and intervention group(n=34).Both groups were exposed to the same comprehensive therapy to control glucose levels.While the control group was given the lower extremity exercise,the intervention group received the extremity exercise in addition to the music media.Result:After three months of intervention,the adherence to the lower extremity exercise regimen in the intervention group was significantly higher than that of the control group(p<0.05).Additionally,following six months of treatment,both the dorsal artery peak values and ankle-brachial indices(ABIs)showed significant differences between the control and intervention groups(p<0.05).Conclusion:Music media treatment combined with lower extremity exercise can both significantly increase the extent of exercise compliance of elderly patients suffering from diabetes mellitus,as well as improve blood circulation in their feet.

Effect of Internet technology on extended care in elderly patients with diabetic feet

Objective： To evaluate the effect of Internet technology on continuing nursing in elderly patients with diabetic feet, Method： From January 2015 to July 2016,12 elderly patients with diabetic foot ulcers were enrolled from the Endocrinology Department in our hospital. We used ＂WeChat＂, ＂E nursing＂ and other Internet technologies to perform remote extended care and to observe the foot ulcer outcomes. Results： All foot ulcers healed with a wound healing time between 38 and 73 days （average 57.08 ~ 12.69 days）. Patients did not need to travel long distances to seek medical treatment for foot ulcers, improving their satisfaction. Conclusions： The implementation of extended care for elderly patients with diabetic foot ulcers was based on the application of Internet technology. It is helpful to facilitate medical treatment, share high quality health resources and promote disease rehabilitation.

Quantum steerability of two qubits mediated by one-dimensional plasmonic waveguides

We study the dynamics of the quantum steering between two separated qubits trapped in a one-dimensional plasmonic waveguide.By numerical methods,we calculate the quantum steerability and other quantum correlations,i.e.,entanglement,discord,and coherence,for both cases with and without laser driving fields.It is found that steerability may exhibit a sudden disappearance and sudden reappearance phenomenon.Specifically,there exist time windows with no steerability but finite entanglement.The effects of plasmon wavenumber and the distance between the two qubits on steerability are also examined.Furthermore,we show that quantum steerability is tunable by adjusting the laser driving fields.

Nursing care for diabetic toe ulcers:A case series report and literature review

This paper reports the treatment of eight cases with toe ulcers.The eight patients received nursing treatments,including debridement,dressings,decompression,anti-fungal management,and education from specialist nurses.The patients were also given drugs to control glucose,nourish the nerve,and improve blood circulation of the lower extremities.The toe ulcers of the eight diabetic patients healed after 42e168 days[average 85±36 days].Thus far,few studies have focused on diabetic toe ulcers.Timely identification of risk factors and regular toe care could decrease the prevalence of toe ulcers and shorten the recovery time in patients with diabetic mellitus.Hence,these patients must be diagnosis and treated early.Results suggest that feet,particularly toes,should be inspected and cared for regularly in diabetic patients.

High-purity Mg and Mg-1Ca alloys:Comparative assessment of the merits regarding degradation,osteogenesis,and biosafety for orthopedic applications

High-purity magnesium(HP Mg)and Mg-1Ca,as representations of Mg-matrix implants produced by purifying and alloying,are employed in biomedical applications primarily because of their bioactivity and degradability.The superiority of both degradation properties,the match between degradation and osteo-genesis in vivo,and biosafety are critical problems that will decide future purifying or alloying to construct Mg-based implants and promote clinical translation.The present study investigated the benefits and limitations of degradation behavior and biosafety of HP Mg and Mg-1Ca according to the electro-chemical experiment,hydrogen evolution test,immersion test,and in vivo bone implantation assay.The results indicated that due to its Mg 2 Ca phase,Mg-1Ca exhibited a considerably higher corrosion current density and hydrogen production than HP Mg in vitro.Furthermore,HP Mg and Mg-1Ca display a favorable match between their degradation and the surrounding osteogenesis,resulting in no significant variation in degradation in vivo during 26 weeks.Additionally,the implantation and degradation of HP Mg and Mg-1Ca do not result in major organ dysfunction or pathological abnormalities.This work is expected to lay the foundation for future clinical translation of Mg and Mg alloy orthopedic implants.

A bioinspired and high-strengthed hydrogel for regeneration of perforated temporomandibular joint disc:Construction and pleiotropic immunomodulatory effects

Due to the lack of an ideal material for TMJ(temporomandibular joint)disc perforation and local inflammation interfering with tissue regeneration,a functional TGI/HA-CS(tilapia type I gelatin/hyaluronic acid-chondroitin sulfate)double network hydrogel was constructed in this paper.It was not only multiply bionic in its composition,structure and mechanical strength,but also endowed with the ability to immunomodulate microenvironment and simultaneously induce in situ repair of defected TMJ discs.On the one hand,it inhibited inflammatory effects of inflammasome in macrophages,reduced the extracellular matrix(ECM)-degrading enzymes secreted by chondrocytes,reversed the local inflammatory state,promoted the proliferation of TMJ disc cells and induced fibrochondrogenic differentiation of synovium-derived mesenchymal stem cells(SMSCs).On the other hand,it gave an impetus to repairing a relatively-large(6 mm-sized)defect in mini pigs’TMJ discs in a rapid and high-quality manner,which suggested a promising clinical application.

高度近视萎缩弧面积的多模态成像定量分析

目的:比较高度近视患者萎缩弧(PPA)面积的多模态成像定量分析结果。方法:系列病例研究。收集2016年10月至2017年10月在首都医科大学附属北京友谊医院就诊的高度近视伴萎缩弧的患者30例(45眼)。其中,男13例(19眼),女17例(26眼)。年龄27~69(53.1±11.6)岁。所有患者均行眼底彩色照相(CFP)、近红外自发荧光(NIR-FAF)、眼底荧光素血管造影(FFA)及频域光学相干断层扫描(OCT)检查。由2名眼科医师在相互独立、互不干扰的情况下分别对上述多模态成像图像进行精准定量分析。采用Image J 1.50i版软件描绘并计算CFP、FAF、FFA图像中PPA的面积。当NIR-FAF难以确定视盘和PPA边界时,用频域OCT辅助确定。医师之间多模态成像结果的一致性检验采用Bland-Altman分析。结果:CFP、NIR-FAF、FFA检查测得患眼PPA面积分别为(7.1±4.7)mm2、(8.3±5.8)mm2、(8.1±5.5)mm2。CFP、FAF、FFA 3种方法测得PPA面积差异无统计学意义(F=1.193,P=0.307)。Bland-Altman分析结果显示,2名眼科医师之间多模态成像结果的一致性较高,其变异范围CFP<NIR-FAF<FFA。结论:NIR-FAF测得高度近视PPA面积最大,FFA次之,CFP最小。

多环芳烃厌氧生物降解研究进展

多环芳烃(PAHs)是环境中广泛分布的一类持久性有机污染物,对生态环境和公众健康具有极大危害。微生物降解是环境中去除多环芳烃污染的有效途径,近年来PAHs厌氧生物降解研究逐渐取代好氧降解成为人们关注的重点。本文从PAHs厌氧生物降解的研究背景出发,从不同厌氧还原反应体系、厌氧降解微生物、PAHs厌氧生物转化途径等方面阐述了PAHs厌氧生物降解的研究概况,归纳了对PAHs厌氧生物降解有积极作用的影响因素,提出了PAHs厌氧降解研究目前存在的问题,并对该领域未来研究方向作了简述和展望。希望为多环芳烃厌氧生物降解与环境修复研究与实践提供参考。

Up-conversion fluorescence biosensor for sensitive detection of CA-125 tumor markers

Sensitive and specific bioassays of tumor markers are critical for early cancer detection and treatment.In recent years,lanthanide(Ln3+)doped upconversion nanoparticles(UCNPs)have attracted wide attentions in tumor markers detectio n.Herein,we co mbined NaYF4:Yb,Tm and silver nanoparticles,serving as energy donor and receptor,respectively,to form an up-conversion fluorescence based inhibitory tumor marker biosensor system.The tumor marker CA-125 molecules are labeled with silver NPs,and the energy transfer fluorescent signal can be detected between the UCNPs and the silver NP receptors.The biosensor shows good stability,high sensitivity and selectivity in the tumor marker concentration range from 5 to 100 ng/mL,as well as a detection limit of 120 pg/mL.Due to the advantage of ease of fabrication and operation,low cost and high information capacity,this technology holds great potential for the clinical applications.

Anticancer activity of Isoliquiritigenin: biological effects and molecular mechanisms

Natural compounds derived from plants have attracted considerable attention in the scientific community due to their nontoxic nature and anti-cancer activity.Isoliquiritigenin(ISL),an active flavonoid isolated from the root of the licorice plant(Glycyrrhiza uralensis),has been previously demonstrated to have anti-inflammatory,antioxidant and tumor suppressive effects.In the past few years,the number of studies describing the effects of ISL against cancer has been gradually increased.ISL has been found to inhibit viability,proliferation,and migration of cancer cells mainly through cell cycle arrest,induction of apoptosis as well as autophagy.However,the molecular mechanisms of action are not completely comprehended.This review aimed to provide a comprehensive summary of the biological effects and molecular mechanisms of ISL against cancer.

Removal of Cu（Ⅱ） and Fe（Ⅲ） from aqueous solutions by dead sulfate reducing bacteria

The biosorption properties of dead sulfate reducing bacteria （SRB） for the removal of Cu（Ⅱ） and Fe（Ⅲ） from aqueous solutions was studied. The effects of the biosorbent concentration, the initial pH value and the temperature on the biosorption of Cu（Ⅱ） and Fe（Ⅲ） by the SRB were investigated. FTIR analysis verified that the hydroxyl, carbonyl and amine functional groups of the SRB biosorbent were involved in the biosorption process. For both Cu（Ⅱ） and Fe（Ⅲ）, an increase in the SRB biosorbent concentration resulted in an increase in the removal percentage but a decrease in the amount of specific metal biosorption. The maximum specific metal biosorption was 93.25 mg·g^-1 at pH 4.5 for Cu（Ⅱ） and 88.29 mg· g^-1 at pH 3.5 for Fe（Ⅲ）. The temperature did not have a significant effect on biosorption. In a binary metal system, the specific biosorption capacity for the target metal decreased when another metal ion was added. For both the single metal and binary metal systems, the biosorption of Cu（Ⅱ） and Fe（Ⅲ） onto a SRB biosorbent was better represented by a Langmuir model than by a Freundlich model.