Despite being a common therapy for hepatocellular carcinoma(HCC),insufficient thermal ablation can leave behind tumor residues that can cause recurrence.This is believed to augment M2 inflammatory macrophages that usu...Despite being a common therapy for hepatocellular carcinoma(HCC),insufficient thermal ablation can leave behind tumor residues that can cause recurrence.This is believed to augment M2 inflammatory macrophages that usually play a pro-tumorigenic role.To address this problem,we designed D-mannose-chelated iron oxide nanoparticles(man-IONPs) to polarize M2-like macrophages into the antitumor Ml phenotype.In vitro and in vivo experiments demonstrated that man-IONPs specifically targeted M2-like macrophages and accumulated in peri-ablation zones after macrophage infiltration was augmented under insufficient microwave ablation(MWA).The nanoparticles simultaneously induced polarization of pro-tumorigenic M2 macrophages into antitumor M1 phenotypes,enabling the transformation of the immunosuppressive microenvironment into an immunoactivating one.Post-MWA macrophage polarization exerted robust inhibitory effects on HCC progression in a well-established orthotopic liver cancer mouse model.Thus,combining thermal ablation with man-IONPs can salvage residual tumors after insufficient MWA.These results have strong potential for clinical translation.展开更多
Lacustrine shale oil resources in China are abundant,with remarkable exploration breakthroughs being achieved.Compared to marine shale oil in North America,efficient exploration of lacustrine shale oil is more difficu...Lacustrine shale oil resources in China are abundant,with remarkable exploration breakthroughs being achieved.Compared to marine shale oil in North America,efficient exploration of lacustrine shale oil is more difficult;thus,selecting favorable layer and optimization zone for horizontal wells is more important.In this study,based on systematic coring of approximately 500 m fine-grained deposits of the Kong 2 Member,combining laboratory tests and log data,source rock geochemistry and reservoir physical properties,the favorable rock fabric facies for oil accumulation was analyzed and classified.First,the dominant lithologic facies,organic facies,and bed combination facies were determined based on mineral composition from logging,total organic content(TOC),and sedimentary structure.Secondly,10 fabric facies were classified by combining these three facies,with 4 fabric facies were found to have high TOC content,high total hydrocarbon,and strong fluorescence features,indicating good shale oil enrichment.Thirdly,the distribution of the upon good fabric facies was identified to be located at the top of the Kong 2 Member,with evidences of seismic resistivity inversion,thermal maturity,structure depth,and strata thickness.And the favorable facies were found to be stably distributed lateral at the area of about 100 km2.High oil flow has been detected at this layer within this area by several wells,including horizontal wells.The exploratory study of fabric facies classification and evaluation provides a new research idea for lacustrine shale oil exploration and effectively promotes breakthroughs in lacustrine shale oil exploration in Bohai Bay Basin.展开更多
基金supported by the National Natural Science Foundation of China (Grant Nos. 91859201, 92159305, 81971625, and 82030047)
文摘Despite being a common therapy for hepatocellular carcinoma(HCC),insufficient thermal ablation can leave behind tumor residues that can cause recurrence.This is believed to augment M2 inflammatory macrophages that usually play a pro-tumorigenic role.To address this problem,we designed D-mannose-chelated iron oxide nanoparticles(man-IONPs) to polarize M2-like macrophages into the antitumor Ml phenotype.In vitro and in vivo experiments demonstrated that man-IONPs specifically targeted M2-like macrophages and accumulated in peri-ablation zones after macrophage infiltration was augmented under insufficient microwave ablation(MWA).The nanoparticles simultaneously induced polarization of pro-tumorigenic M2 macrophages into antitumor M1 phenotypes,enabling the transformation of the immunosuppressive microenvironment into an immunoactivating one.Post-MWA macrophage polarization exerted robust inhibitory effects on HCC progression in a well-established orthotopic liver cancer mouse model.Thus,combining thermal ablation with man-IONPs can salvage residual tumors after insufficient MWA.These results have strong potential for clinical translation.
基金supported by PetroChina Major Science and Technology Project“Research and Application of Key Technology for Efficient Reservoir Increase and Stable Production in Dagang Oil and Gas Field”(2018E-11)。
文摘Lacustrine shale oil resources in China are abundant,with remarkable exploration breakthroughs being achieved.Compared to marine shale oil in North America,efficient exploration of lacustrine shale oil is more difficult;thus,selecting favorable layer and optimization zone for horizontal wells is more important.In this study,based on systematic coring of approximately 500 m fine-grained deposits of the Kong 2 Member,combining laboratory tests and log data,source rock geochemistry and reservoir physical properties,the favorable rock fabric facies for oil accumulation was analyzed and classified.First,the dominant lithologic facies,organic facies,and bed combination facies were determined based on mineral composition from logging,total organic content(TOC),and sedimentary structure.Secondly,10 fabric facies were classified by combining these three facies,with 4 fabric facies were found to have high TOC content,high total hydrocarbon,and strong fluorescence features,indicating good shale oil enrichment.Thirdly,the distribution of the upon good fabric facies was identified to be located at the top of the Kong 2 Member,with evidences of seismic resistivity inversion,thermal maturity,structure depth,and strata thickness.And the favorable facies were found to be stably distributed lateral at the area of about 100 km2.High oil flow has been detected at this layer within this area by several wells,including horizontal wells.The exploratory study of fabric facies classification and evaluation provides a new research idea for lacustrine shale oil exploration and effectively promotes breakthroughs in lacustrine shale oil exploration in Bohai Bay Basin.
