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乏情和发情初产母猪下丘脑–垂体–卵巢轴中lincRNAs表达谱比较分析 被引量:3
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作者 任巧玲 张家庆 +7 位作者 陆东锋 王璟 陈俊峰 马强 白献晓 郭红霞 高彬文 邢宝松 《遗传》 CAS CSCD 北大核心 2020年第4期388-402,共15页
初产母猪断奶后能否正常发情对养猪生产影响重大,也是初产母猪被淘汰的主要原因。本研究以乏情和发情初产母猪为研究对象,首次利用RNA-seq技术对其下丘脑-垂体-卵巢轴中的基因间长链非编码RNAs(long intergenic noncoding RNAs,lincRNAs... 初产母猪断奶后能否正常发情对养猪生产影响重大,也是初产母猪被淘汰的主要原因。本研究以乏情和发情初产母猪为研究对象,首次利用RNA-seq技术对其下丘脑-垂体-卵巢轴中的基因间长链非编码RNAs(long intergenic noncoding RNAs,lincRNAs)进行筛选比较,得到lincRNAs的表达图谱,并对其特征和功能进行了初步分析。结果显示,在乏情和发情初产母猪下丘脑–垂体–卵巢轴中鉴定得到3519个lincRNAs,以发情组为对照共有17个lincRNAs存在差异表达,其中12个表达上调,5个表达下调(FC≥2,P<0.05)。选择4个差异表达的lincRNAs经qRT-PCR验证,其表达水平与测序结果基本一致。对这17个差异表达的lincRNAs进行GO分析、KEGG通路分析及lincRNA-mRNA共表达网络分析,发现这些lincRNAs主要与猪卵母细胞减数分裂成熟、卵巢细胞分化及颗粒细胞凋亡等生殖活动相关。本研究结果丰富了猪lincRNAs数据资源,为进一步深入研究初产母猪的生殖机能提供了理论依据。 展开更多
关键词 lincRNAs 乏情 发情 初产母猪 下丘脑–垂体–卵巢轴
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去势和非去势公猪背最长肌circRNA差异表达分析 被引量:1
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作者 邢宝松 王璟 +7 位作者 陈俊峰 马强 任巧玲 张家庆 张华 滑留帅 孙加节 曹海 《遗传》 CAS CSCD 北大核心 2021年第11期1066-1077,共12页
公猪去势可减少异味和打斗,但去势后产肉量和肌内脂肪沉积发生变化,其分子机制的解析对生产具有重要意义。近年来研究表明,环状RNA(circRNA)在肌肉发育中具有重要调控作用。为探究去势后circRNAs对背最长肌发育的调控,本研究选择6头淮... 公猪去势可减少异味和打斗,但去势后产肉量和肌内脂肪沉积发生变化,其分子机制的解析对生产具有重要意义。近年来研究表明,环状RNA(circRNA)在肌肉发育中具有重要调控作用。为探究去势后circRNAs对背最长肌发育的调控,本研究选择6头淮南公猪,随机选择3头去势,当体重达130 kg左右屠宰,采集背最长肌样品,利用高通量测序筛选差异表达circRNAs(differentially expressed circRNAs,DECs)并进行KEGG功能富集分析。结合前期筛选的公猪去势相关miRNAs,构建DECs-miRNAs调控网络,最后使用猪骨骼肌卫星细胞验证候选circRNA表达谱及其与miRNA互作关系。结果表明,去势和非去势组背最长肌样品共获得5866个circRNAs,两组之间共有370个DECs(|log2Foldchange|>1,padj<0.8),KEGG富集分析表明,DECs来源母基因主要富集于肌肉发育、肌纤维类型转化、能量代谢等相关通路。构建的DECs-miRNA调控网络共包括69个circRNAs和8个miRNAs。选择circRNA_2241和circRNA_4237进行验证,结果发现这两个circRNAs真实存在且表达趋势与测序结果一致。进一步在猪骨骼肌卫星细胞初步验证circRNA_2241与miR-1互作关系,结果表明睾酮显著促进circRNA_2241表达,同时抑制miR-1表达。本研究结果提示circRNAs可能通过与miRNAs互作调控猪去势后背最长肌发育,从而为解析去势对肌肉发育调控的分子机制提供参考。 展开更多
关键词 环状RNA 去势 公猪 背最长肌
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Ophthalmic complications related to dengue fever: a case report
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作者 Ruihong Ju Jinglin zhang +2 位作者 jiaqing zhang Binbin Wu Yipeng Deng 《Eye Science》 CAS 2016年第3期178-181,共4页
Two patients aged of 30 and 22(female in cases 1,and male in case 2) both complained of unilateral blurring of vision and scotoma within a week of being diagnosed with dengue fever.No other abnormal findings were foun... Two patients aged of 30 and 22(female in cases 1,and male in case 2) both complained of unilateral blurring of vision and scotoma within a week of being diagnosed with dengue fever.No other abnormal findings were found in their anterior segment.Retinal examination revealed blurring of the optic disc margin and several white spots in the posterior in both cases.Optical coherence tomography(OCT)imagery revealed that the white spots were only located in the retinal outer layers.Macular cystic foveolitis were also found in case 1 and diffused macular edema in case 2.In case 1,visual and retinal recovery were seen to resolve spontaneously.In case 2,patient had complete visual recovery two months after onset of the disease after being treated with steroids but central scotomata has continued to persist. 展开更多
关键词 Dengue fever ophthalmic complications foveolitis optical coherence tomography(OCT)
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Path of Promoting Industrial Transformation and Upgrading Based on Rural Revitalization:A Case Study of Green Plum Industry in Luhe County of Shanwei City
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作者 jiaqing zhang Peiling MA Qian JIANG 《Asian Agricultural Research》 2022年第8期9-12,共4页
Taking green plum Industry in Luhe County of Shanwei City in Guangdong Province as an example,this paper made a precise positioning,based on the characteristics of rural tourism resources in Yongchuan Township,took th... Taking green plum Industry in Luhe County of Shanwei City in Guangdong Province as an example,this paper made a precise positioning,based on the characteristics of rural tourism resources in Yongchuan Township,took the current situation as the guide and improving the tourism function as the carrier,made an innovation of the rural tourism industry system,in order to improve the quality of rural tourism development,and realize the strategic goal of rural revitalization. 展开更多
关键词 Green plum industry Rural revitalization Luhe County
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全固态钠离子电池及界面改性
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作者 杨冬荣 张达 +5 位作者 任昆 李付鹏 东鹏 张家庆 杨斌 梁风 《化学进展》 SCIE CAS CSCD 北大核心 2023年第8期1177-1190,共14页
全固态钠离子电池具有原料成本低、安全性高以及能量密度高等特点,在移动电源、电动汽车和大规模储能系统领域表现出巨大的应用潜力。然而全固态钠离子电池的发展和规模化应用亟需解决固体电解质室温离子电导率低、界面电荷转移阻抗大... 全固态钠离子电池具有原料成本低、安全性高以及能量密度高等特点,在移动电源、电动汽车和大规模储能系统领域表现出巨大的应用潜力。然而全固态钠离子电池的发展和规模化应用亟需解决固体电解质室温离子电导率低、界面电荷转移阻抗大、固体电解质与电极界面兼容性和接触差等问题。本文结合近年来全固态钠离子电池相关报道和本课题组研究成果,概述了β-Al2O3型固体电解质、NASICON型固体电解质、硫化物固体电解质、聚合物固体电解质、复合固体电解质的研究进展及发展趋势;综述了全固态钠离子电池界面特性、固体电解质表面修饰、电极/固体电解质界面改性最新研究成果;最后对全固态钠离子电池界面改性策略发展方向进行了展望。本综述有助于加深对全固态钠离子电池界面科学问题的认识,并对固态钠离子电池的发展应用形成理论指导。 展开更多
关键词 全固态钠离子电池 固体电解质 界面 改性
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Experimental Study on Elevated Fires in a Ceiling Vented Compartment 被引量:3
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作者 jiaqing zhang Shouxiang LU +3 位作者 Qiang LI Changhai LI Man YUAN Richard YUEN 《Journal of Thermal Science》 SCIE EI CAS CSCD 2013年第4期377-382,共6页
The impacts of elevation on fires in a ceiling vented compartment were investigated experimentally. The flame behavior of elevated fires was recorded. Various parameters including the fuel mass loss rate, the light ex... The impacts of elevation on fires in a ceiling vented compartment were investigated experimentally. The flame behavior of elevated fires was recorded. Various parameters including the fuel mass loss rate, the light extinction coefficient, the oxygen concentration and the gas temperature were measured. Results indicated that the variations of the flame behavior were consistent with that of the fuel mass loss rate. The fire location significantly impacted the light extinction coefficient, the oxygen concentration and the gas temperature, which all showed distinct stratification phenomena. For a higher elevated fire, the average fuel loss rate and the overall light extinction coefficient were smaller, the oxygen concentration was higher and the gas temperature was lower. In addition, the smoke descending was slower. From the perspective of those parameters the fire was less hazardous if the fire was elevated higher, which was totally different from the elevated fires in closed compartments. 展开更多
关键词 火灾危险性 天花板 高架 实验 通风 质量损失率 气体温度 货厢
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Large eddy simulation of room fire spread using a medium scale compartment made of medium density fibreboard (MDF) panels 被引量:1
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作者 Jiayidaer Baolati Kaiyuan Li +4 位作者 Yanyan Zou Kevin Frank George Hare jiaqing zhang Fanliang Ge 《Building Simulation》 SCIE EI CSCD 2022年第4期495-510,共16页
At present,there is a shortage of experimental and simulation studies on fire spread in medium-and large-scale compartments while the existing models of the fire spread are limited for typical engineering applications... At present,there is a shortage of experimental and simulation studies on fire spread in medium-and large-scale compartments while the existing models of the fire spread are limited for typical engineering applications.This paper proposes a new model for large-scale fire spread on medium density fibreboard(MDF)panels.Validating the model with single burning item(SBI)experiments,it is found that the numerical simulation closely predicts the experimental heat release rate(HRR)with some error near the peak.The predicted heat flux and distance of lateral flame spread are consistent with the experiments and an existing model.The effects of kinetic properties and heat of combustion are identified through a sensitivity analysis.The decrease of activation energy and increase of pre-exponential factor make the MDF easier to pyrolyze and the increase of heat of combustion enhances the flame temperature and thus provide more heat feedback to the sample surface.The low activation energy(71.9 kJ/mol)and high heat of combustion(46.5 MJ/kg)of the model ensure the occurrence of flame spread.Furthermore,the model was validated using medium-scale compartment fire experiments and the results showed that the model can accurately predict the HRR after flashover(the error is within 7%).While the burner is ignited,the predictions of in-compartment gas temperature and heat flux are more accurate.However,when the burner is extinguished,the modelled in-compartment gas temperature is lower than the experimental values,resulting in a lower heat flux prediction.The model leads to easier flame spread;therefore,the modelled flame spreads faster in the compartment compared to the experiment,and thus the HRR increases more rapidly. 展开更多
关键词 flame spread compartment fire SBI numerical simulation MDF activation energy
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Construction of physical crosslink-based chitosan/liquid crystal composite hydrogel and evaluation on their cytocompatibility
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作者 Lin Du Xiaohui Yang +4 位作者 Wenqiang Li Xuhui Luo Hao Wu jiaqing zhang Mei Tu 《Regenerative Biomaterials》 SCIE 2017年第1期39-45,共7页
In order to provide a novel biomimetic composite substrate for tissue engineering and explore the interaction between cells and this type of material,we developed chitosan/liquid crystal(CS/LC)composite hydrogel with ... In order to provide a novel biomimetic composite substrate for tissue engineering and explore the interaction between cells and this type of material,we developed chitosan/liquid crystal(CS/LC)composite hydrogel with embedded LC phases by composing of cholesterol hydroxypropyl cellulose ester liquid crystalline material and CS.The micromorphology of CS/LC composite hydrogels exhibited‘islands-sea’phase separation structures similar to the‘fluid mosaic model’of biomembrane.In vitro cell compatibility study suggested that 3T3 is fibroblasts exhibited better initial cell adhesions and higher proliferation rates on the composite hydrogel than on the polystyrene control plate and the pure LC membrane.This novel CS/LC composite hydrogel provides more favorable interface for cell growth and proliferation and may serve as potentially active substrate for engineering interfaces to live cells. 展开更多
关键词 CHITOSAN liquid crystal composite hydrogels structure and morphology cell compatibility
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