[Objectives]To investigate the serum metabolomic characteristics of primary dysmenorrhea rat model induced by estradiol benzoate combined with oxytocin,and to reveal its material basis.[Methods]20 female SD rats were ...[Objectives]To investigate the serum metabolomic characteristics of primary dysmenorrhea rat model induced by estradiol benzoate combined with oxytocin,and to reveal its material basis.[Methods]20 female SD rats were randomly divided into control group and model group.The primary dysmenorrhea rat model was established by subcutaneous injection of estradiol benzoate for 10 consecutive days and intraperitoneal injection of oxytocin on the last day.The serum samples of rats in control group and model group were collected by ultra-performance liquid chromatography(UPLC)quadrupole time-of-flight mass spectrometry(Q-TOF-MS).The differential metabolites were identified by multivariable pattern recognition method and endogenous metabolite database,and the metabolic pathways were enriched by Metaboanalyst 5.0 platform.[Results]There were significant differences in serum metabolic profiles between the two groups.A total of 36 potential biomarkers of primary dysmenorrhea including L-tyrosine,glycocholic acid,citric acid,palmitoyl carnitine and cholesterol were screened and identified,mainly involving metabolic pathways such as phenylalanine,tyrosine and tryptophan biosynthesis,glycerophospholipid metabolism,and primary bile acid biosynthesis.[Conclusions]The serum metabolic profile of primary dysmenorrhea rats deviates significantly from that of healthy rats,and there are multiple metabolic pathway disorders,which are mainly related to phenylalanine,tyrosine and tryptophan biosynthesis,glycerophospholipid metabolism,and primary bile acid biosynthesis.展开更多
基金Supported by the Natural Science Foundation for the Youth funded by the Natural Science Foundation of China(82104384)the Science and Technology Project of Hebei Education Department(QN2021008)+5 种基金the Research Fund for the Doctoral Program Funded by the Chengde Medical University(202103)Key Discipline Construction Project of Colleges and Universities in Hebei Province(Ji Jiao Gao[2013]4)Technology Innovation Guidance Project-Science and Technology Work Conference of Hebei Provincial Department of Science and TechnologyPharmacodynamic Material Basis Team of Traditional Chinese Medicine of the Youth PI(Principle Investigator)Science and Technology Innovation Team Project of Chengde Medical UniversityChengde Medical University Student Innovation and Entrepreneurship Training Program(202203)Hebei Key Laboratory of Nerve Injury and Repair(NJKF202202)。
文摘[Objectives]To investigate the serum metabolomic characteristics of primary dysmenorrhea rat model induced by estradiol benzoate combined with oxytocin,and to reveal its material basis.[Methods]20 female SD rats were randomly divided into control group and model group.The primary dysmenorrhea rat model was established by subcutaneous injection of estradiol benzoate for 10 consecutive days and intraperitoneal injection of oxytocin on the last day.The serum samples of rats in control group and model group were collected by ultra-performance liquid chromatography(UPLC)quadrupole time-of-flight mass spectrometry(Q-TOF-MS).The differential metabolites were identified by multivariable pattern recognition method and endogenous metabolite database,and the metabolic pathways were enriched by Metaboanalyst 5.0 platform.[Results]There were significant differences in serum metabolic profiles between the two groups.A total of 36 potential biomarkers of primary dysmenorrhea including L-tyrosine,glycocholic acid,citric acid,palmitoyl carnitine and cholesterol were screened and identified,mainly involving metabolic pathways such as phenylalanine,tyrosine and tryptophan biosynthesis,glycerophospholipid metabolism,and primary bile acid biosynthesis.[Conclusions]The serum metabolic profile of primary dysmenorrhea rats deviates significantly from that of healthy rats,and there are multiple metabolic pathway disorders,which are mainly related to phenylalanine,tyrosine and tryptophan biosynthesis,glycerophospholipid metabolism,and primary bile acid biosynthesis.
