Identification of serous ovarian tumors based on polarization imaging and correlation analysis with clinicopathological features

Ovarian cancer is one of the most aggressive and heterogeneous female tumors in the world,and serous ovarian cancer(SOC)is of particular concern for being the leading cause of ovarian cancer death.Due to its clinical and biological complexities,ovarian cancer is still considered one of the most di±cult tumors to diagnose and manage.In this study,three datasets were assembled,including 30 cases of serous cystadenoma(SCA),30 cases of serous borderline tumor(SBT),and 45 cases of serous adenocarcinoma(SAC).Mueller matrix microscopy is used to obtain the polarimetry basis parameters(PBPs)of each case,combined with a machine learning(ML)model to derive the polarimetry feature parameters(PFPs)for distinguishing serous ovarian tumor(SOT).The correlation between the mean values of PBPs and the clinicopathological features of serous ovarian cancer was analyzed.The accuracies of PFPs obtained from three types of SOT for identifying dichotomous groups(SCA versus SAC,SCA versus SBT,and SBT versus SAC)were 0.91,0.92,and 0.8,respectively.The accuracy of PFP for identifying triadic groups(SCA versus SBT versus SAC)was 0.75.Correlation analysis between PBPs and the clinicopathological features of SOC was performed.There were correlations between some PBPs(δ,β,q_(L),E_(2),rqcross,P_(2),P_(3),P_(4),and P_(5))and clinicopathological features,including the International Federation of Gynecology and Obstetrics(FIGO)stage,pathological grading,preoperative ascites,malignant ascites,and peritoneal implantation.The research showed that PFPs extracted from polarization images have potential applications in quantitatively differentiating the SOTs.These polarimetry basis parameters related to the clinicopathological features of SOC can be used as prognostic factors.

Hierarchically porous N-doped carbon derived from biomass as oxygen reduction electrocatalyst for high-performance Al–air battery

Developing large-scale and highly efficient oxygen reduction reaction(ORR)catalysts acts a vital role in realizing wide application of metal–air batteries.Here,we propose a gas-foaming strategy to fabricate sustainable and 3D hierarchically porous N-doped carbon with high specific surface area and abundant defects sites derived from biomass.The obtained catalyst exhibits prominent ORR property with higher half-wave potential(0.861 V)and slightly lower kinetic current density(32.44 m A cm^-2),compared to Pt/C(0.856 V and 43.61 m A cm^-1).Furthermore,employing it as catalyst of air cathode,the Al–air battery delivers remarkable discharge performance with excellent power density of 401 m W cm^-2,distinguished energy density of 2453.4 Wh kg^-1 and extremely high open-circuit voltage of 1.85 V among the reported metal–air batteries in the literatures.This gas-foaming strategy for full utilization of biomass affords a chance to explore scalable advanced catalysts in metal–air battery.

Brassinosteroid and gibberellin coordinate rice seed germination and embryo growth by regulating glutelin mobilization

Seed germination is the beginning of a new lifecycle,and involves many complex physiological and biochemical reactions including seed reserve mobilization in the endosperm and nutrient transport and reuse in the embryo.Although glutelin is a dominant storage protein in rice,its contribution to seed germination and its regulatory mechanisms are mostly unknown.Gibberellin (GA) and brassinosteroid (BR),two major growth-promoting phytohormones,also play positive roles in controlling seed germination.However,how GA and BR interact and coordinate seed germination and facilitate glutelin mobilization remains unclear.In the present study,biochemical and physiological analyses of seed germination indicated that both GA and BR promote seed germination and post-germination growth.Exogenous application of GA restored germination defects caused by BR deficiency or insensitivity.Proteomic and q RT-PCR results showed that the expression of several glutelin proteins and their encoding genes was induced by BR and GA in the embryo.Expression assays suggested that the increased accumulation of glutelin protein in the embryo was due to the accelerated degradation of glutelin by a cysteine proteinase (REP-1) in the endosperm.The breakdown of glutelin in the endosperm showed a strict positive correspondence with the length of the shoot.The GluA2 mutation led to reduced degradation rate of glutelin and defects in seed germination,and the promotion effect of GA on seed germination was weakened in the glua2mutant.In vitro culture assay of rice embryos showed that glutelin mobilization functioned downstream of the GA and BR pathways to promote shoot elongation.These findings suggest a mechanism that mediates crosstalk between BR and GA in co-regulating rice seed germination and embryo growth.

Effect and Its Mechanism of Brassinolide-regulated Starch Metabolism in Rice Germination

The study was aimed to explore the effect and mechanism of brassinolide（ BL） regulated starch metabolism in rice endosperm during seed germination.The radicle elongation of rice seeds treated with 1 μmol/L BL was inhibited during germination. The analysis on rice seeds with a GUS-fused promoter showed that BL had different regulatory effects on Wx,SBEI,and AGPS1. The effect of BL treatment on the physicochemical properties of rice starch was further investigated by scanning electron microscopy,Fourier transform infrared spectroscopy,and X-ray diffraction. The results showed that the starch treated with BL maintained better crystallinity and orderly structure,indicating that during the germination process,the degradation of starch in endosperm was slow,which might be one of the reasons for the slow radicle growth in the early stages of seed germination. This study provided important clues for further analysis of the molecular mechanisms underlying BR-regulated rice seed germination.

Design and Optimization of Wing Structure for a Fixed-Wing Unmanned Aerial Vehicle (UAV)

The wing, one of the most important parts of aircraft, always requires sophis-ticated design to increase lift, reduce drag and weight. For modern fixed-wing UAV, extending cruising time is always a requirement for the overall design. Designing a most light wing that can match the requirements of work condi-tions is desired. In this work, according to the work conditions, we compare several types of wing and chose beam-type wing. Then we made the detailed design and optimization to reduce the weight of wing. At last, we draw the 3D model for potential realistic production.

miR172-AP2模块调控植物生长发育及逆境响应的研究进展

MicroRNA(miRNA)是一类具有调控能力的非编码小分子RNA,通过与靶基因mRNA特异或非特异性结合,诱导靶基因mRNA降解或抑制其翻译,从而调控植物的生长发育。其中,miR172的靶基因AP2所编码的转录因子为植物所特有,miR172在转录后或翻译水平对AP2进行表达调控,进而调控植物的花发育、时序转换、小穗形态、块茎和果实发育、结瘤(豆科)以及逆境响应等过程。该文综述了近年来miR172-AP2模块在植物生长发育调控方面的最新研究进展。

自体髌骨股四头肌肌腱治疗家兔巨大肩袖损伤的实验研究

目的探究应用自体髌骨股四头肌肌腱移植物修复家兔巨大肩袖损伤愈合效果的实验研究。方法将36只成年健康家兔随机分为3组,分别为对照组(n=12)、缝线修复组(n=12)、移植物修复组(n=12)。对照组切开一侧肩关节,暴露肩袖肌腱后缝合伤口;缝线修复组采用经骨缝合方法将切断的肩袖肌腱缝合至肱骨大结节;移植物修复组将自体髌骨股四头肌肌腱移植物缝合至肩袖破裂处,髌骨固定于肱骨大结节,股四头肌肌腱固定于关节盂。术后6周各组处死6只家兔进行生物力学测试,术后8周每组处死6只家兔进行组织形态学分析。结果生物力学研究结果显示移植物修复组的拉力最大值明显大于缝线修复组(P<0.05),其与对照组无明显差异。组织形态学分析显示移植物修复组髌骨与肱骨愈合良好;缝线修复组可见缝合部位腱骨愈合不佳,与肱骨连接处纤维组织较为疏松。结论自体髌骨股四头肌肌腱修复家兔巨大肩袖损伤可在肱骨处以骨-骨愈合方式取代腱-骨愈合,达到更好的愈合强度。