Reinventing the tetraploid potato into a seed-propagated,diploid,hybrid potato would significantly accelerate potato breeding.In this regard,the development of highly homozygous inbred lines is a prerequisite for bree...Reinventing the tetraploid potato into a seed-propagated,diploid,hybrid potato would significantly accelerate potato breeding.In this regard,the development of highly homozygous inbred lines is a prerequisite for breeding hybrid potatoes,but self-incompatibility and inbreeding depression present challenges for developing pure inbred lines.To resolve this impediment,we developed a doubled haploid(DH)technology,based on mutagenesis of the potato DOMAIN OF UNKNOWN FUNCTION 679 membrane protein(StDMP)gene.Here,we show that a deficiency in StDMP allows the generation of maternal haploids for generating diploid potato lines.An exercisable protocol,involving hybridization,fluorescent marker screening,molecular and flow cytometric identification,and doubling with colchicine generates nearly 100%homozygous diploid potato lines.This dmp-triggered haploid induction(HI)system greatly shortens the breeding process and offers a robust method for generating diploid potato inbred lines with high purity.展开更多
To study the change mechanism and the control of the variable cycle engine in the process of modal transition,a variable cycle engine model based on component level characteristics is established.The two-dimensional C...To study the change mechanism and the control of the variable cycle engine in the process of modal transition,a variable cycle engine model based on component level characteristics is established.The two-dimensional CFD technology is used to simulate the influence of mode selection valve rotation on the engine flow field,which improves the accuracy of the model.Furthermore,the constant flow control plan is proposed in the modal transition process to reduce the engine installed drag.The constant flow control plan adopts the augmentation linear quadratic regulator control method.Simulation results indicate that the control method is able to effectively control the bypass ratio and demand flow of the variable cycle engine,and make the engine transform smoothly,which ensures the stable operation of the engine in modal transition and the constant demand flow of the engine.展开更多
基金supported by the Key Project of the Major Research Base of Humanities and Social Sciences of the Ministry of Education (22JJD720022)the Social Science Foundation Project of Fujian Province (FJ2023C072)the College Students' Innovation and Entrepreneurship Training Program Project of Guangdong Province (S202111078016)。
基金supported by the Guangdong Major Project of Basic and Applied Basic Research(2021B0301030004)the National Natural Science Foundation of China(Grant No.31991185,31902027)supported by the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Science.Author information。
文摘Reinventing the tetraploid potato into a seed-propagated,diploid,hybrid potato would significantly accelerate potato breeding.In this regard,the development of highly homozygous inbred lines is a prerequisite for breeding hybrid potatoes,but self-incompatibility and inbreeding depression present challenges for developing pure inbred lines.To resolve this impediment,we developed a doubled haploid(DH)technology,based on mutagenesis of the potato DOMAIN OF UNKNOWN FUNCTION 679 membrane protein(StDMP)gene.Here,we show that a deficiency in StDMP allows the generation of maternal haploids for generating diploid potato lines.An exercisable protocol,involving hybridization,fluorescent marker screening,molecular and flow cytometric identification,and doubling with colchicine generates nearly 100%homozygous diploid potato lines.This dmp-triggered haploid induction(HI)system greatly shortens the breeding process and offers a robust method for generating diploid potato inbred lines with high purity.
基金co-supported by the National Science and Technology Major Project, China (No. J2019-Ⅲ-0009-0053)the Advanced Jet Propulsion Creativity Center, China (No. HKCX2020020022)
文摘To study the change mechanism and the control of the variable cycle engine in the process of modal transition,a variable cycle engine model based on component level characteristics is established.The two-dimensional CFD technology is used to simulate the influence of mode selection valve rotation on the engine flow field,which improves the accuracy of the model.Furthermore,the constant flow control plan is proposed in the modal transition process to reduce the engine installed drag.The constant flow control plan adopts the augmentation linear quadratic regulator control method.Simulation results indicate that the control method is able to effectively control the bypass ratio and demand flow of the variable cycle engine,and make the engine transform smoothly,which ensures the stable operation of the engine in modal transition and the constant demand flow of the engine.
