Virus-induced gene silencing(VIGS)is a natural defense mechanism of plants,which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome.At present,the VIGS system med...Virus-induced gene silencing(VIGS)is a natural defense mechanism of plants,which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome.At present,the VIGS system mediated by Tobacco rattle virus(TRV)has been successfully established in many plant species.However,the VIGS system in Chinese narcissus had not been reported yet.In this paper,the first use of the TRV-VIGS system in Chinese narcissus is described in detail.By injecting pTRV2-GFP into narcissus leaves,we confirmed that TRV could infect narcissus and move in narcissus plants.After the inoculation of pTRV2-Nt PDS by two methods,phenomena such as photo-bleaching appeared in narcissus leaves and qRT-PCR results proved that PDS gene of narcissus was successfully silenced.Besides,pTRV2-NtMYB3 was inoculated into basal plates of narcissus to study the function of NtMYB3;the results showed that NtMYB3 is an inhibitor of flavonoids biosynthesis,which can increase the content of proanthocyanidins in basal plates of narcissus by repressing the expression level of NtFLS.These results indicate that the TRV-VIGS system has been successfully established in Chinese narcissus and successfully applied to the functional study of NtMYB3.展开更多
Iron hexacyanoferrate(FeHCF)is a promising cathode material for sodium-ion batteries.However,FeHCF always suffers from a poor cycling stability,which is closely related to the abundant vacancy defects in its framework...Iron hexacyanoferrate(FeHCF)is a promising cathode material for sodium-ion batteries.However,FeHCF always suffers from a poor cycling stability,which is closely related to the abundant vacancy defects in its framework.Herein,post-synthetic and in-situ vacancy repairing strategies are proposed for the synthesis of highquality FeHCF in a highly concentrated Na_(4)Fe(CN)_(6) solution.Both the post-synthetic and in-situ vacancy repaired FeHCF products(FeHCF-P and FeHCF-I)show the significant decrease in the number of vacancy defects and the reinforced structure,which can suppress the side reactions and activate the capacity from low-spin Fe in FeHCF.In particular,FeHCF-P delivers a reversible discharge capacity of 131 mAh g^(−1) at 1 C and remains 109 mAh g^(−1) after 500 cycles,with a capacity retention of 83%.FeHCF-I can deliver a high discharge capacity of 158.5 mAh g^(−1) at 1 C.Even at 10 C,the FeHCF-I electrode still maintains a discharge specific capacity of 103 mAh g^(−1) and retains 75% after 800 cycles.This work provides a new vacancy repairing strategy for the solution synthesis of high-quality FeHCF.展开更多
[Objectives]To systematically study the effects of different nitrogen levels on the growth and nitrogen utilization of sugarcane in Guangxi.[Methods]Through field experiment and indoor analysis,different nitrogen appl...[Objectives]To systematically study the effects of different nitrogen levels on the growth and nitrogen utilization of sugarcane in Guangxi.[Methods]Through field experiment and indoor analysis,different nitrogen application levels were set up to determine soil nitrogen content and sugarcane nitrogen content.The effects of different nitrogen levels on sugarcane yield,agronomic characters and nitrogen utilization were studied.[Results]The effect of nitrogen application rate on sugarcane yield showed a quadratic curve,and nitrogen application could significantly increase sugarcane yield,and the sugarcane yield reached the maximum when the nitrogen application rate reached 714 kg/ha.[Conclusions]With the increase of nitrogen application rate,sugarcane yield increased,but when it exceeded a certain range,the sugarcane yield decreased significantly.展开更多
[Objectives]This study was conducted to investigate the correlation between leaf blight of pearl plum and nutrient elements from the perspective of plant nutrition.[Methods]Branch and leaf samples of pearl plum plants...[Objectives]This study was conducted to investigate the correlation between leaf blight of pearl plum and nutrient elements from the perspective of plant nutrition.[Methods]Branch and leaf samples of pearl plum plants infected by or free of leaf blight and the corresponding 0-30 cm cultivated soil were collected,and statistical analysis was conducted according to the changes in nutrient contents and the incidence of leaf blight,so as to look for nutrients in pearl plum plants associated with leaf blight,as well as soil constraint factors.[Results]Phosphorus,iron and manganese in nutrients may be related to leaf blight,and the factors affecting leaf blight may be the ratios of P/Fe and P/Mn in leaves.[Conclusions]This study provides a basis for further research on the relationship between phosphorus,iron and manganese on the effect of leaf blight in pearl plum trees.展开更多
基金the Science and technology innovation fund of Fujian Agriculture and Forestry University(Grant Nos.KFA17352A,KFA17602A)。
文摘Virus-induced gene silencing(VIGS)is a natural defense mechanism of plants,which can cause sequence-specific degradation of viral RNA and is often used for studying the functional genome.At present,the VIGS system mediated by Tobacco rattle virus(TRV)has been successfully established in many plant species.However,the VIGS system in Chinese narcissus had not been reported yet.In this paper,the first use of the TRV-VIGS system in Chinese narcissus is described in detail.By injecting pTRV2-GFP into narcissus leaves,we confirmed that TRV could infect narcissus and move in narcissus plants.After the inoculation of pTRV2-Nt PDS by two methods,phenomena such as photo-bleaching appeared in narcissus leaves and qRT-PCR results proved that PDS gene of narcissus was successfully silenced.Besides,pTRV2-NtMYB3 was inoculated into basal plates of narcissus to study the function of NtMYB3;the results showed that NtMYB3 is an inhibitor of flavonoids biosynthesis,which can increase the content of proanthocyanidins in basal plates of narcissus by repressing the expression level of NtFLS.These results indicate that the TRV-VIGS system has been successfully established in Chinese narcissus and successfully applied to the functional study of NtMYB3.
基金supported by the projects of the National Key R&D Program of China(2016YFB0100302)the National Natural Science Foundation of China(Grant No.60306011).
文摘Iron hexacyanoferrate(FeHCF)is a promising cathode material for sodium-ion batteries.However,FeHCF always suffers from a poor cycling stability,which is closely related to the abundant vacancy defects in its framework.Herein,post-synthetic and in-situ vacancy repairing strategies are proposed for the synthesis of highquality FeHCF in a highly concentrated Na_(4)Fe(CN)_(6) solution.Both the post-synthetic and in-situ vacancy repaired FeHCF products(FeHCF-P and FeHCF-I)show the significant decrease in the number of vacancy defects and the reinforced structure,which can suppress the side reactions and activate the capacity from low-spin Fe in FeHCF.In particular,FeHCF-P delivers a reversible discharge capacity of 131 mAh g^(−1) at 1 C and remains 109 mAh g^(−1) after 500 cycles,with a capacity retention of 83%.FeHCF-I can deliver a high discharge capacity of 158.5 mAh g^(−1) at 1 C.Even at 10 C,the FeHCF-I electrode still maintains a discharge specific capacity of 103 mAh g^(−1) and retains 75% after 800 cycles.This work provides a new vacancy repairing strategy for the solution synthesis of high-quality FeHCF.
基金Guangxi Key R&D Program(GuiKe AB18221027)Guangxi Natural Science Foundation(2018GXNSFAA281027)Basic Scientific Research Program of Guangxi Academy of Agricultural Sciences(2020YM110,2021YT036).
文摘[Objectives]To systematically study the effects of different nitrogen levels on the growth and nitrogen utilization of sugarcane in Guangxi.[Methods]Through field experiment and indoor analysis,different nitrogen application levels were set up to determine soil nitrogen content and sugarcane nitrogen content.The effects of different nitrogen levels on sugarcane yield,agronomic characters and nitrogen utilization were studied.[Results]The effect of nitrogen application rate on sugarcane yield showed a quadratic curve,and nitrogen application could significantly increase sugarcane yield,and the sugarcane yield reached the maximum when the nitrogen application rate reached 714 kg/ha.[Conclusions]With the increase of nitrogen application rate,sugarcane yield increased,but when it exceeded a certain range,the sugarcane yield decreased significantly.
基金Supported by Guangxi Key Research and Development Project(GK AB18294010)Special Project of Basic Scientific Research Business of Guangxi Academy of Agricultural Sciences(GNK 2021YT036)Key Project of Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(GNK 2016JZ09)。
文摘[Objectives]This study was conducted to investigate the correlation between leaf blight of pearl plum and nutrient elements from the perspective of plant nutrition.[Methods]Branch and leaf samples of pearl plum plants infected by or free of leaf blight and the corresponding 0-30 cm cultivated soil were collected,and statistical analysis was conducted according to the changes in nutrient contents and the incidence of leaf blight,so as to look for nutrients in pearl plum plants associated with leaf blight,as well as soil constraint factors.[Results]Phosphorus,iron and manganese in nutrients may be related to leaf blight,and the factors affecting leaf blight may be the ratios of P/Fe and P/Mn in leaves.[Conclusions]This study provides a basis for further research on the relationship between phosphorus,iron and manganese on the effect of leaf blight in pearl plum trees.