In recent EAST experiments,current profile broadening characterized by reduced internal inductance has been achieved by utilizing radio-frequency current drives(RFCD).In contrast to previous density scan experiments,w...In recent EAST experiments,current profile broadening characterized by reduced internal inductance has been achieved by utilizing radio-frequency current drives(RFCD).In contrast to previous density scan experiments,which showed an outward shift of the current density profile of lower hybrid current drive(LHCD)in higher plasma density,the core electron temperature(T_(e)(0))is found to affect the LHCD current profile as well.According to equilibrium reconstruction,a significant increase in on-axis safety factor(q0)from 2.05 to 3.41 is observed by careful arrangement of RFCD.Simulations using ray-tracing code GENRAY and Fokker–Planck code CQL3D have been performed to thoroughly analyze the LHCD current profile,revealing the sensitivity of the LHCD current profile to T_(e)(0).The LHCD current density tends to accumulate in the plasma core with higher current drive efficiency benefiting from higher T_(e)(0).With a lower T_(e)(0),the LHCD current profile broadens due to off-axis deposition of power density.The sensitivity of the power deposition and current profile of LHCD to T_(e)(0)provides a promising way to effectively optimize current profile via control of the core electron temperature.展开更多
Resonant magnetic perturbations(RMPs)with high toroidal mode number n are considered for controlling edge-localized modes(ELMs)and divertor heat flux in future ITER H-mode operations.In this paper,characteristics of d...Resonant magnetic perturbations(RMPs)with high toroidal mode number n are considered for controlling edge-localized modes(ELMs)and divertor heat flux in future ITER H-mode operations.In this paper,characteristics of divertor heat flux under high-nRMPs(n=3 and 4)in H-mode plasma are investigated using newly upgraded infrared thermography diagnostic in EAST.Additional splitting strike point(SSP)accompanying with ELM suppression is observed under both RMPs with n=3 and n=4,the SSP in heat flux profile agrees qualitatively with the modeled magnetic footprint.Although RMPs suppress ELMs,they increase the stationary heat flux during ELM suppression.The dependence of heat flux on q_(95)during ELM suppression is preliminarily investigated,and further splitting in the original strike point is observed at q 495=during ELM suppression.In terms of ELM pulses,the presence of RMPs shows little influence on transient heat flux distribution.展开更多
Objective:To explore the correlations between five-pattern personality scores and autonomic nervous response indicators in Chinese female college students,to provide a foundation for further exploration of the modern ...Objective:To explore the correlations between five-pattern personality scores and autonomic nervous response indicators in Chinese female college students,to provide a foundation for further exploration of the modern physiological basis of these personality types.Methods:Subjects were asked to fill in 'The Five-Pattern Personality Inventory' (revised edition 2008).Taiyang,Shaoyang,yin-yang balance,Taiyin,and Shaoyin personalities were scored.The galvanic skin response and heart rate,and the low frequency (LF),high frequency (HF),and LF/HF ratio of heart rate variability were collected using a 16-channel physiological recorder (BIOPAC MP150).Relationship between the five-pattern personality scores and autonomic nervous response indicators was analyzed using bivariate correlation.Results:The five-pattern personality scores of the subjects were compared with national averages.The Taiyang and Shaoyang personality scores of the subjects were significantly lower than the overall national average,the national female average,and the national female average in the 18-29-year-old age group (all P <.05).The Taiyang personality score was negatively correlated with both the LF and the HF (P =.009 and P =.001,respectively),and the yin-yang balance personality score was significantly negatively correlated with the galvanic skin response (P =.026).Conclusion:There is a relationship between five-pattern personality scores and autonomic nervous response indicators in Chinese female college students.The higher the Taiyang personality score,the lower the sympathetic and vagus nerve excitability;the higher the yinyang balance personality score,the lower the sympathetic nerve excitability.展开更多
Polyoxometalates (POMs) are important inorganic photochromic materials to be potentially applied in photo-induced switch,energy storage,and even the detection of light.However,due to the limited sensitivity of POMs,it...Polyoxometalates (POMs) are important inorganic photochromic materials to be potentially applied in photo-induced switch,energy storage,and even the detection of light.However,due to the limited sensitivity of POMs,it is difficult to realize the photochromic response to weak visible light.In this paper,by the coordination of solvated Pb(Ⅱ),a new structure-defined chain-like polyoxomolybdate complex of[(Pb(DMF)_(4))^(3)(P_(2)Mo_(18)O_(62))_(2)]n(Pb_(3)Mo_(18) ,DMF=dimethylformamide) has been demonstrated by a facile solvent-diffusion approach.By virtue of interactions between Pb(DMF)_(4) and polyoxoanions,Pb_(3)Mo_(18) shows an ultrasensitive photochromic response to weak visible lights and forms the reduced’heteropoly blue’species through ligand-to-metal charge transfer (LMCT) process.A new mechanism is firstly proposed here that the 6s orbital lone electron pair on Pb(II) can effectively stabilize the generated hole of oxygen atoms as a result of O→Mo charge transfer.Through the proposed mechanism,the LMCT barrier is drastically lowered and allows the coloration to be occurred even upon weak visible light.Also,because the conductivity of Pb_(3)Mo_(18) enhances with the increase of reduction extent,its electrochemical impedance signals are proportionally response to irradiation intensity.Especially,for the first time,the polyoxomolybdate composite can be used to detect weak visible light,in which the optical signal can be converted into electrical signal output.Moreover,Pb_(3)Mo_(18) can be drip-coated on the surface of the screen printed chip electrode,which is facile to the detection of light by portable devices compatible with computers,mobile phones and other electronic equipment.This work not only highlights a new approach to the molecular design of photochromic POMs by the coordination of metal ions with the effect of inert electron pair,but also lays a foundation to extend the application of POMs as light signal sensors.展开更多
Objective:Diterpenoids with a wide variety of biological activities from Anoectochilus roxburghii,a precious medicinal plant,are important active components.However,due to the lack of genetic information on the metabo...Objective:Diterpenoids with a wide variety of biological activities from Anoectochilus roxburghii,a precious medicinal plant,are important active components.However,due to the lack of genetic information on the metabolic process of diterpenoids in A.roxburghii,the genes involved in the molecular regulation mechanism of diterpenoid metabolism are still unclear.This study revealed the complex metabolic genes for diterpenoids biosynthesis in different organs of A.roxburghii by combining analysis of transcriptomics and metabolomics.Methods:The differences in diterpenoid accumulation in roots,stems and leaves of A.roxburghii were analyzed by metabonomic analysis,and its metabolic gene information was obtained by transcriptome sequencing.Then,the molecular mechanism of differential diterpenoid accumulation in different organs of A.roxburghii was analyzed from the perspective of gene expression patterns.Results:A total of 296 terpenoid metabolites were identified in the five terpenoid metabolic pathways in A.roxburghii.There were 38,34,and 18 diterpenoids with different contents between roots and leaves,between leaves and stems,and between roots and stems,respectively.Twenty-nine metabolic enzyme genes with 883 unigenes in the diterpenoid synthesis process were identified,and the DXS and FDPS in the terpenoid backbone biosynthesis stage and CPA,GA20ox,GA3ox,GA2ox,and MAS in the diterpenoid biosynthesis stage were predicted to be the key metabolic enzymes for the accumulation of diterpenoids.In addition,14 key transcription factor coding genes were predicted to be involved in the regulation of the diterpenoid biosynthesis.The expression of genes such as GA2ox,MAS,CPA,GA20ox and GA3ox might be activated by some of the 14 transcription factors.The transcription factor NTF-Y and PRE6 were predicted to be the most important transcription factors.Conclusion:This study determined 29 metabolic enzyme genes and predicted 14 transcription factors involved in the molecular regulation mechanism of diterpenoid metabolism in A.roxburghii,which provided a reference for the further study of the molecular regulation mechanism of the accumulation of diterpenoids in different organs of A.roxburghii.展开更多
As a representative of chronic wounds,the long-term high levels of oxidative stress and blood sugar in chronic diabetic wounds lead to serious complications,making them the biggest challenge in the research on wound h...As a representative of chronic wounds,the long-term high levels of oxidative stress and blood sugar in chronic diabetic wounds lead to serious complications,making them the biggest challenge in the research on wound healing.Many edible natural biomaterials rich in terpenes,phenols,and flavonoids can act as efficient antioxidants.In this study,okra extract was selected as the main component of a wound dressing.The okra extracts obtained via different methods comprehensively maintained the bioactivity of multiple molecules.The robust antioxidant properties of okra significantly reduced intracellular reactive oxygen species production,thereby accelerating the wound healing process.The results showed that okra extracts and their hydrogel dressings increased cell migration,angiogenesis,and re-epithelization of the chronic wound area,considerably promoting wound remodeling in diabetic rats.Therefore,okra-based hydrogels are promising candidates for skin regeneration and wider tissue engineering applications.展开更多
Pseudo-Particle Modeling (PPM) is a particle method proposed by Ge and Li in 1996 [Ge, W., & Li, J. (1996). Pseudo-particle approach to hydrodynamics of particle-fluid systems, in M. Kwauk & J. Li (Eds.), Proc...Pseudo-Particle Modeling (PPM) is a particle method proposed by Ge and Li in 1996 [Ge, W., & Li, J. (1996). Pseudo-particle approach to hydrodynamics of particle-fluid systems, in M. Kwauk & J. Li (Eds.), Proceedings of the 5th international conference on drculating fluidized bed (pp. 260-265). Beijing: Science Press] and has been used to explore the microscopic mechanism in complex particle-fluid systems. But as a particle method, high computational cost remains a main obstacle for its large-scale application; therefore, parallel implementation of this method is highly desirable. Parallelization of two-dimensional PPM was carried out by spatial decomposition in this paper. The time costs of the major functions in the program were analyzed and the program was then optimized for higher efficiency by dynamic load balancing and resetting of particle arrays. Finally, simulation on a gas-solid fluidized bed with 102,400 solid particles and 1.8 × 10^7 pseudo-particles was performed successfully with this code, indicating its scalability in future applications.展开更多
cAMP is an important second messenger that is capable of controlling a wide array of cellular processes, including glycogen, sugar and lipid metabolism. Here we report the design and construction of a novel geneticall...cAMP is an important second messenger that is capable of controlling a wide array of cellular processes, including glycogen, sugar and lipid metabolism. Here we report the design and construction of a novel genetically encoded fluorescent sensor for cAMP. The sensor was realized by fusing E. coli CAP protein with cpYFP, and displayed a one-fold fluorescence change towards c AMP binding. Further characterization assays demonstrated that the sensor had high affinity for cAMP and fast response kinetics.The development of our sensor could be a useful supplement to existing methods for cAMP detection.展开更多
基金supported by the National MCF Energy R&D Program of China (No. 2019YFE0304000)National Natural Science Foundation of China (Nos. 12005262 and 11975274)+3 种基金the Anhui Provincial Natural Science Foundation (No. 2108085J06)the Users with Excellence Program of Hefei Science Center CAS (Nos. 2021HSC-UE018 and 2020HSC-UE011)the External Cooperation Program of Chinese Academy of Sciences (No. 116134KYSB20180035)the Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences (No. DSJJ-2021-04)
文摘In recent EAST experiments,current profile broadening characterized by reduced internal inductance has been achieved by utilizing radio-frequency current drives(RFCD).In contrast to previous density scan experiments,which showed an outward shift of the current density profile of lower hybrid current drive(LHCD)in higher plasma density,the core electron temperature(T_(e)(0))is found to affect the LHCD current profile as well.According to equilibrium reconstruction,a significant increase in on-axis safety factor(q0)from 2.05 to 3.41 is observed by careful arrangement of RFCD.Simulations using ray-tracing code GENRAY and Fokker–Planck code CQL3D have been performed to thoroughly analyze the LHCD current profile,revealing the sensitivity of the LHCD current profile to T_(e)(0).The LHCD current density tends to accumulate in the plasma core with higher current drive efficiency benefiting from higher T_(e)(0).With a lower T_(e)(0),the LHCD current profile broadens due to off-axis deposition of power density.The sensitivity of the power deposition and current profile of LHCD to T_(e)(0)provides a promising way to effectively optimize current profile via control of the core electron temperature.
基金supported by the National Key Research and Development Program of China (No. 2017YFA0402500)the National MCF Energy R&D Program of China (No. 2019YFE03040000)+5 种基金National Natural Science Foundation of China (Nos. 12005262 and 11975274)the Foundation of President of Hefei Institutes of Physical Science, CAS (No. YZJJ2018QN8)the Anhui Provincial Natural Science Foundation (No. 2108085J06)the Users with Excellence Program of Hefei Science Center CAS (Nos. 2021HSC-UE018 and 2020HSC-UE011)External Cooperation Program of Chinese Academy of Sciences (No. 116134KYSB20180035)Science Foundation of Institute of Plasma Physics, Chinese Academy of Sciences (No. DSJJ-2021-04)
文摘Resonant magnetic perturbations(RMPs)with high toroidal mode number n are considered for controlling edge-localized modes(ELMs)and divertor heat flux in future ITER H-mode operations.In this paper,characteristics of divertor heat flux under high-nRMPs(n=3 and 4)in H-mode plasma are investigated using newly upgraded infrared thermography diagnostic in EAST.Additional splitting strike point(SSP)accompanying with ELM suppression is observed under both RMPs with n=3 and n=4,the SSP in heat flux profile agrees qualitatively with the modeled magnetic footprint.Although RMPs suppress ELMs,they increase the stationary heat flux during ELM suppression.The dependence of heat flux on q_(95)during ELM suppression is preliminarily investigated,and further splitting in the original strike point is observed at q 495=during ELM suppression.In terms of ELM pulses,the presence of RMPs shows little influence on transient heat flux distribution.
基金This study was supported by the National Natural Science Foundation of China(81473598).
文摘Objective:To explore the correlations between five-pattern personality scores and autonomic nervous response indicators in Chinese female college students,to provide a foundation for further exploration of the modern physiological basis of these personality types.Methods:Subjects were asked to fill in 'The Five-Pattern Personality Inventory' (revised edition 2008).Taiyang,Shaoyang,yin-yang balance,Taiyin,and Shaoyin personalities were scored.The galvanic skin response and heart rate,and the low frequency (LF),high frequency (HF),and LF/HF ratio of heart rate variability were collected using a 16-channel physiological recorder (BIOPAC MP150).Relationship between the five-pattern personality scores and autonomic nervous response indicators was analyzed using bivariate correlation.Results:The five-pattern personality scores of the subjects were compared with national averages.The Taiyang and Shaoyang personality scores of the subjects were significantly lower than the overall national average,the national female average,and the national female average in the 18-29-year-old age group (all P <.05).The Taiyang personality score was negatively correlated with both the LF and the HF (P =.009 and P =.001,respectively),and the yin-yang balance personality score was significantly negatively correlated with the galvanic skin response (P =.026).Conclusion:There is a relationship between five-pattern personality scores and autonomic nervous response indicators in Chinese female college students.The higher the Taiyang personality score,the lower the sympathetic and vagus nerve excitability;the higher the yinyang balance personality score,the lower the sympathetic nerve excitability.
基金the financial support from the National Natural Science Foundation of China (No. 21971085)the Natural Science Foundation of Shandong Province (No. ZR2019MB004)。
文摘Polyoxometalates (POMs) are important inorganic photochromic materials to be potentially applied in photo-induced switch,energy storage,and even the detection of light.However,due to the limited sensitivity of POMs,it is difficult to realize the photochromic response to weak visible light.In this paper,by the coordination of solvated Pb(Ⅱ),a new structure-defined chain-like polyoxomolybdate complex of[(Pb(DMF)_(4))^(3)(P_(2)Mo_(18)O_(62))_(2)]n(Pb_(3)Mo_(18) ,DMF=dimethylformamide) has been demonstrated by a facile solvent-diffusion approach.By virtue of interactions between Pb(DMF)_(4) and polyoxoanions,Pb_(3)Mo_(18) shows an ultrasensitive photochromic response to weak visible lights and forms the reduced’heteropoly blue’species through ligand-to-metal charge transfer (LMCT) process.A new mechanism is firstly proposed here that the 6s orbital lone electron pair on Pb(II) can effectively stabilize the generated hole of oxygen atoms as a result of O→Mo charge transfer.Through the proposed mechanism,the LMCT barrier is drastically lowered and allows the coloration to be occurred even upon weak visible light.Also,because the conductivity of Pb_(3)Mo_(18) enhances with the increase of reduction extent,its electrochemical impedance signals are proportionally response to irradiation intensity.Especially,for the first time,the polyoxomolybdate composite can be used to detect weak visible light,in which the optical signal can be converted into electrical signal output.Moreover,Pb_(3)Mo_(18) can be drip-coated on the surface of the screen printed chip electrode,which is facile to the detection of light by portable devices compatible with computers,mobile phones and other electronic equipment.This work not only highlights a new approach to the molecular design of photochromic POMs by the coordination of metal ions with the effect of inert electron pair,but also lays a foundation to extend the application of POMs as light signal sensors.
基金This work was financially supported by grants from the Department of Technology and Science of the Fujian Provincial Government(No.2021J01132,No.2020J01732)the Fujian Agriculture and Forestry University Science and Technology Innovation Special Fund(No.KFA18071A).
文摘Objective:Diterpenoids with a wide variety of biological activities from Anoectochilus roxburghii,a precious medicinal plant,are important active components.However,due to the lack of genetic information on the metabolic process of diterpenoids in A.roxburghii,the genes involved in the molecular regulation mechanism of diterpenoid metabolism are still unclear.This study revealed the complex metabolic genes for diterpenoids biosynthesis in different organs of A.roxburghii by combining analysis of transcriptomics and metabolomics.Methods:The differences in diterpenoid accumulation in roots,stems and leaves of A.roxburghii were analyzed by metabonomic analysis,and its metabolic gene information was obtained by transcriptome sequencing.Then,the molecular mechanism of differential diterpenoid accumulation in different organs of A.roxburghii was analyzed from the perspective of gene expression patterns.Results:A total of 296 terpenoid metabolites were identified in the five terpenoid metabolic pathways in A.roxburghii.There were 38,34,and 18 diterpenoids with different contents between roots and leaves,between leaves and stems,and between roots and stems,respectively.Twenty-nine metabolic enzyme genes with 883 unigenes in the diterpenoid synthesis process were identified,and the DXS and FDPS in the terpenoid backbone biosynthesis stage and CPA,GA20ox,GA3ox,GA2ox,and MAS in the diterpenoid biosynthesis stage were predicted to be the key metabolic enzymes for the accumulation of diterpenoids.In addition,14 key transcription factor coding genes were predicted to be involved in the regulation of the diterpenoid biosynthesis.The expression of genes such as GA2ox,MAS,CPA,GA20ox and GA3ox might be activated by some of the 14 transcription factors.The transcription factor NTF-Y and PRE6 were predicted to be the most important transcription factors.Conclusion:This study determined 29 metabolic enzyme genes and predicted 14 transcription factors involved in the molecular regulation mechanism of diterpenoid metabolism in A.roxburghii,which provided a reference for the further study of the molecular regulation mechanism of the accumulation of diterpenoids in different organs of A.roxburghii.
基金Thanks to the following fund projects for their support to this work:National Natural Science Foundation of China(Nos.51973243 and 52173150)International Cooperation and Exchange of the National Natural Science Foundation of China(No.51820105004)Shenzhen Basic Research Project(No.JCYJ20190807155801657).
文摘As a representative of chronic wounds,the long-term high levels of oxidative stress and blood sugar in chronic diabetic wounds lead to serious complications,making them the biggest challenge in the research on wound healing.Many edible natural biomaterials rich in terpenes,phenols,and flavonoids can act as efficient antioxidants.In this study,okra extract was selected as the main component of a wound dressing.The okra extracts obtained via different methods comprehensively maintained the bioactivity of multiple molecules.The robust antioxidant properties of okra significantly reduced intracellular reactive oxygen species production,thereby accelerating the wound healing process.The results showed that okra extracts and their hydrogel dressings increased cell migration,angiogenesis,and re-epithelization of the chronic wound area,considerably promoting wound remodeling in diabetic rats.Therefore,okra-based hydrogels are promising candidates for skin regeneration and wider tissue engineering applications.
基金the Designated Funding for Winners of President’s Awards of Chinese Academy of Sciences(CAS,2006)financial supports from the National Natural Science Foundation of China(NSFC)under the Grant No.20221603 and 20706057
文摘Pseudo-Particle Modeling (PPM) is a particle method proposed by Ge and Li in 1996 [Ge, W., & Li, J. (1996). Pseudo-particle approach to hydrodynamics of particle-fluid systems, in M. Kwauk & J. Li (Eds.), Proceedings of the 5th international conference on drculating fluidized bed (pp. 260-265). Beijing: Science Press] and has been used to explore the microscopic mechanism in complex particle-fluid systems. But as a particle method, high computational cost remains a main obstacle for its large-scale application; therefore, parallel implementation of this method is highly desirable. Parallelization of two-dimensional PPM was carried out by spatial decomposition in this paper. The time costs of the major functions in the program were analyzed and the program was then optimized for higher efficiency by dynamic load balancing and resetting of particle arrays. Finally, simulation on a gas-solid fluidized bed with 102,400 solid particles and 1.8 × 10^7 pseudo-particles was performed successfully with this code, indicating its scalability in future applications.
基金National Basic Research Foundation of China(Grant No.2017YFA0505202)the National Natural Science Foundation of China(Grant No.91853107)
文摘cAMP is an important second messenger that is capable of controlling a wide array of cellular processes, including glycogen, sugar and lipid metabolism. Here we report the design and construction of a novel genetically encoded fluorescent sensor for cAMP. The sensor was realized by fusing E. coli CAP protein with cpYFP, and displayed a one-fold fluorescence change towards c AMP binding. Further characterization assays demonstrated that the sensor had high affinity for cAMP and fast response kinetics.The development of our sensor could be a useful supplement to existing methods for cAMP detection.