邻二氮菲合铁(Ⅱ)组成及稳定常数测定实验的改进与优化

“分光光度法测定邻二氮菲合铁(Ⅱ)组成及稳定常数”是化学类及相关专业广泛开设的经典实验。随着各高校实验条件的完善和进步,有必要对此实验进行系统化设计,以适应对学生培养质量的进一步提升要求。本文中,利用紫外-可见分光光度计测试配合物的紫外-可见吸收曲线,确定检测波长;结合配合物的前线轨道对配位显色机理进行解释。利用Origin软件对实验数据进行分析,得到准确、可靠的实验结果。通过内容的改进与扩充,本实验有助于提高教学训练的系统性与综合性,实现对学生科学思维能力的培养。

Impact of core electron temperature on current profile broadening with radiofrequency wave heating and current drive in EAST

In recent EAST experiments,current profile broadening characterized by reduced internal inductance has been achieved by utilizing radio-frequency current drives(RFCD).In contrast to previous density scan experiments,which showed an outward shift of the current density profile of lower hybrid current drive(LHCD)in higher plasma density,the core electron temperature(T_(e)(0))is found to affect the LHCD current profile as well.According to equilibrium reconstruction,a significant increase in on-axis safety factor(q0)from 2.05 to 3.41 is observed by careful arrangement of RFCD.Simulations using ray-tracing code GENRAY and Fokker–Planck code CQL3D have been performed to thoroughly analyze the LHCD current profile,revealing the sensitivity of the LHCD current profile to T_(e)(0).The LHCD current density tends to accumulate in the plasma core with higher current drive efficiency benefiting from higher T_(e)(0).With a lower T_(e)(0),the LHCD current profile broadens due to off-axis deposition of power density.The sensitivity of the power deposition and current profile of LHCD to T_(e)(0)provides a promising way to effectively optimize current profile via control of the core electron temperature.

Study on divertor heat flux under n=3 and n=4 resonant magnetic perturbations using infrared thermography diagnostic in EAST

Resonant magnetic perturbations(RMPs)with high toroidal mode number n are considered for controlling edge-localized modes(ELMs)and divertor heat flux in future ITER H-mode operations.In this paper,characteristics of divertor heat flux under high-nRMPs(n=3 and 4)in H-mode plasma are investigated using newly upgraded infrared thermography diagnostic in EAST.Additional splitting strike point(SSP)accompanying with ELM suppression is observed under both RMPs with n=3 and n=4,the SSP in heat flux profile agrees qualitatively with the modeled magnetic footprint.Although RMPs suppress ELMs,they increase the stationary heat flux during ELM suppression.The dependence of heat flux on q_(95)during ELM suppression is preliminarily investigated,and further splitting in the original strike point is observed at q 495=during ELM suppression.In terms of ELM pulses,the presence of RMPs shows little influence on transient heat flux distribution.

Correlations between five-pattern personality scores from traditional Chinese medicine and autonomic nervous response indicators in healthy female college students

Objective:To explore the correlations between five-pattern personality scores and autonomic nervous response indicators in Chinese female college students,to provide a foundation for further exploration of the modern physiological basis of these personality types.Methods:Subjects were asked to fill in 'The Five-Pattern Personality Inventory' (revised edition 2008).Taiyang,Shaoyang,yin-yang balance,Taiyin,and Shaoyin personalities were scored.The galvanic skin response and heart rate,and the low frequency (LF),high frequency (HF),and LF/HF ratio of heart rate variability were collected using a 16-channel physiological recorder (BIOPAC MP150).Relationship between the five-pattern personality scores and autonomic nervous response indicators was analyzed using bivariate correlation.Results:The five-pattern personality scores of the subjects were compared with national averages.The Taiyang and Shaoyang personality scores of the subjects were significantly lower than the overall national average,the national female average,and the national female average in the 18-29-year-old age group (all P <.05).The Taiyang personality score was negatively correlated with both the LF and the HF (P =.009 and P =.001,respectively),and the yin-yang balance personality score was significantly negatively correlated with the galvanic skin response (P =.026).Conclusion:There is a relationship between five-pattern personality scores and autonomic nervous response indicators in Chinese female college students.The higher the Taiyang personality score,the lower the sympathetic and vagus nerve excitability;the higher the yinyang balance personality score,the lower the sympathetic nerve excitability.

双侧岩下窦采血指导下神经内镜手术治疗库欣病一例报道

库欣综合征(Cushing's syndrome,CS)又称皮质醇增多症,是由多种病因引起的以高皮质醇血症为特征的临床综合征,当促肾上腺皮质激素(adrenocorticotropic hormone,ACTH)分泌来源为垂体肿瘤时,即为库欣病(Cushing's disease,CD);当分泌来源位于肾上腺或肺部等部位时,则为异位ACTH综合征(ectopic ACTH syndrome,EAS)。典型的皮质醇增多症临床表现包括满月脸、水牛背、多血质外貌、皮肤紫纹[1]。双侧岩下窦采血(bilateral inferior petrosal sinus sampling,BIPSS)是目前鉴别诊断CD和EAS最可靠的方法,被认为是诊断CD的金标准,尤其适用于影像学无法明确的微腺瘤的诊断。本文报道1例在BIPSS指导下神经内镜成功切除微腺瘤的CD患者,并结合相关文献总结治疗经验,以期为CD的鉴别与诊断提供参考。

Ultrasensitive photochromism and impedance dual response to weak visible light by solvated Pb(Ⅱ) modified polyoxomolybdate

Polyoxometalates (POMs) are important inorganic photochromic materials to be potentially applied in photo-induced switch,energy storage,and even the detection of light.However,due to the limited sensitivity of POMs,it is difficult to realize the photochromic response to weak visible light.In this paper,by the coordination of solvated Pb(Ⅱ),a new structure-defined chain-like polyoxomolybdate complex of[(Pb(DMF)_(4))^(3)(P_(2)Mo_(18)O_(62))_(2)]n(Pb_(3)Mo_(18) ,DMF=dimethylformamide) has been demonstrated by a facile solvent-diffusion approach.By virtue of interactions between Pb(DMF)_(4) and polyoxoanions,Pb_(3)Mo_(18) shows an ultrasensitive photochromic response to weak visible lights and forms the reduced’heteropoly blue’species through ligand-to-metal charge transfer (LMCT) process.A new mechanism is firstly proposed here that the 6s orbital lone electron pair on Pb(II) can effectively stabilize the generated hole of oxygen atoms as a result of O→Mo charge transfer.Through the proposed mechanism,the LMCT barrier is drastically lowered and allows the coloration to be occurred even upon weak visible light.Also,because the conductivity of Pb_(3)Mo_(18) enhances with the increase of reduction extent,its electrochemical impedance signals are proportionally response to irradiation intensity.Especially,for the first time,the polyoxomolybdate composite can be used to detect weak visible light,in which the optical signal can be converted into electrical signal output.Moreover,Pb_(3)Mo_(18) can be drip-coated on the surface of the screen printed chip electrode,which is facile to the detection of light by portable devices compatible with computers,mobile phones and other electronic equipment.This work not only highlights a new approach to the molecular design of photochromic POMs by the coordination of metal ions with the effect of inert electron pair,but also lays a foundation to extend the application of POMs as light signal sensors.

Combined analysis of transcriptomics and metabolomics revealed complex metabolic genes for diterpenoids biosynthesis in different organs of Anoectochilus roxburghii

Objective:Diterpenoids with a wide variety of biological activities from Anoectochilus roxburghii,a precious medicinal plant,are important active components.However,due to the lack of genetic information on the metabolic process of diterpenoids in A.roxburghii,the genes involved in the molecular regulation mechanism of diterpenoid metabolism are still unclear.This study revealed the complex metabolic genes for diterpenoids biosynthesis in different organs of A.roxburghii by combining analysis of transcriptomics and metabolomics.Methods:The differences in diterpenoid accumulation in roots,stems and leaves of A.roxburghii were analyzed by metabonomic analysis,and its metabolic gene information was obtained by transcriptome sequencing.Then,the molecular mechanism of differential diterpenoid accumulation in different organs of A.roxburghii was analyzed from the perspective of gene expression patterns.Results:A total of 296 terpenoid metabolites were identified in the five terpenoid metabolic pathways in A.roxburghii.There were 38,34,and 18 diterpenoids with different contents between roots and leaves,between leaves and stems,and between roots and stems,respectively.Twenty-nine metabolic enzyme genes with 883 unigenes in the diterpenoid synthesis process were identified,and the DXS and FDPS in the terpenoid backbone biosynthesis stage and CPA,GA20ox,GA3ox,GA2ox,and MAS in the diterpenoid biosynthesis stage were predicted to be the key metabolic enzymes for the accumulation of diterpenoids.In addition,14 key transcription factor coding genes were predicted to be involved in the regulation of the diterpenoid biosynthesis.The expression of genes such as GA2ox,MAS,CPA,GA20ox and GA3ox might be activated by some of the 14 transcription factors.The transcription factor NTF-Y and PRE6 were predicted to be the most important transcription factors.Conclusion:This study determined 29 metabolic enzyme genes and predicted 14 transcription factors involved in the molecular regulation mechanism of diterpenoid metabolism in A.roxburghii,which provided a reference for the further study of the molecular regulation mechanism of the accumulation of diterpenoids in different organs of A.roxburghii.

Natural okra-based hydrogel for chronic diabetic wound healing

As a representative of chronic wounds,the long-term high levels of oxidative stress and blood sugar in chronic diabetic wounds lead to serious complications,making them the biggest challenge in the research on wound healing.Many edible natural biomaterials rich in terpenes,phenols,and flavonoids can act as efficient antioxidants.In this study,okra extract was selected as the main component of a wound dressing.The okra extracts obtained via different methods comprehensively maintained the bioactivity of multiple molecules.The robust antioxidant properties of okra significantly reduced intracellular reactive oxygen species production,thereby accelerating the wound healing process.The results showed that okra extracts and their hydrogel dressings increased cell migration,angiogenesis,and re-epithelization of the chronic wound area,considerably promoting wound remodeling in diabetic rats.Therefore,okra-based hydrogels are promising candidates for skin regeneration and wider tissue engineering applications.

Parallelization of pseudo-particle modeling and its application in simulating gas-solid fluidization

Pseudo-Particle Modeling （PPM） is a particle method proposed by Ge and Li in 1996 [Ge, W., ＆ Li, J. （1996）. Pseudo-particle approach to hydrodynamics of particle-fluid systems, in M. Kwauk ＆ J. Li （Eds.）, Proceedings of the 5th international conference on drculating fluidized bed （pp. 260-265）. Beijing： Science Press] and has been used to explore the microscopic mechanism in complex particle-fluid systems. But as a particle method, high computational cost remains a main obstacle for its large-scale application; therefore, parallel implementation of this method is highly desirable. Parallelization of two-dimensional PPM was carried out by spatial decomposition in this paper. The time costs of the major functions in the program were analyzed and the program was then optimized for higher efficiency by dynamic load balancing and resetting of particle arrays. Finally, simulation on a gas-solid fluidized bed with 102,400 solid particles and 1.8 × 10^7 pseudo-particles was performed successfully with this code, indicating its scalability in future applications.

基于大肠杆菌CAP蛋白的检测cAMP的新型荧光探针（英文）

cAMP是细胞内一种重要的信号分子,作为第二信使调控包括糖代谢、糖原代谢和脂质代谢在内的一系列生理过程。在本文中我们报道一个新颖的检测细胞内c AMP的基因编码荧光探针,这一探针是由大肠杆菌CAP蛋白和cpYFP荧光蛋白组成的融合蛋白,针对cAMP的结合可以产生一倍的荧光信号响应。进一步的表征实验表明,这一探针对c AMP具有很强的结合能力,并且能够实现快速地荧光响应。这一探针的开发将会为目前已有的检测cAMP的手段提供有益的补充。