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Characterization of cytotoxic compound from mangrove derived fungi Irpex hydnoides VB4 被引量:3
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作者 B Valentin Bhimba DA Agnel Defora Franco +2 位作者 Geena Mary Jose jibi merin mathew Elsa Lycias Joel 《Asian Pacific Journal of Tropical Biomedicine》 SCIE CAS 2011年第3期223-226,共4页
Objective:To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi.Methods:In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers I... Objective:To investigate the cytotoxic activity of endophytic fungi isolated from mangrove fungi.Methods:In the present study the DNA was isolated and the ITS region of 5.8s rRNA was amplified using specific primers ITS 1 and ITS4 and sequence was determined using automated sequencers.Blast search sequence similarity was found against the existing non redundant nucleotide sequence database thus,identified as Aspergilus flavus,Hyporcaea lixii,Aspergillus niger,Eutorium amstelodami,Irpex hydnoides and Neurospora crassa.Among the seven isolates, one fungi Irpex hydnoides was selected for further studies.The fungi were grown in sabouraud broth for five days and filtrate were separated and subjected to ethyl acetate for further studies. Results:Nearly half(49.25%) of the extracts showed activity(IC_(50) of 125 μ g/mL).These values were within the cutoff point of the National Cancer Institute criteria for cytotoxicity(IC_(50)<20 μ g/mL) in the screening of crude plant extracts.The GC MS analysis revealed that the active principals might be Tetradecane(6.26%) with the RT 8.606.Conclusions:It is clear from the present study that mangrove fungi with bioactive metabolites can be expected to provide high quality biological material for high throughout biochemical,anti cancer screening programmes.The results help us conclude that me potential of using metabolic engineering and post genomic approaches to isolate more novel bioactive compounds and to make their possible commercial application is not far off. 展开更多
关键词 MANGROVE FUNGI CYTOTOXIC activity ITS sequencing GC MS Irpex hynoides CHARACTERIZATION CYTOTOXIC COMPOUND Endophytic FUNGI MANGROVE Bioactive metabolite
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红树林真菌Hypocrea lixii VB1的抗癌及抗菌活性(英文)
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作者 B.Valentin Bhimba D.A.Agnel Defora Franco +3 位作者 jibi merin mathew Geena Mary Jose Elsa Lycias Joel M.Thangaraj 《中国天然药物》 SCIE CAS CSCD 2012年第1期77-80,共4页
AIM:Mangrove is one of the oldest living tree species and its leaves are among the most extensively studied botanicals in use today.Scientific research throughout the world has found evidence to support the fact that ... AIM:Mangrove is one of the oldest living tree species and its leaves are among the most extensively studied botanicals in use today.Scientific research throughout the world has found evidence to support the fact that its foliar extracts have great potential against human microbial pathogens.This study highlights the isolation of foliar fungi from Rhizophora mucronata,Avicenna officialis and Avicenna marina.METHOD:It was isolated in Sabouroud's Dextrose Agar and mass cultivation was done in Sabouroud's Dex-trose broth.RESULTS:The ethyl acetate extract showed maximum antibacterial activity which inturn checked for different concentra-tion against bacterial pathogens and anticancer activity for Hep2 and MCF7 cell line in vitro.The DNA was isolated from the fungi and the ITS region of 5.8 s RNA was sequenced and assigned to new species as they are separated from the type strains phylogenetic neighbors by sequence similarities.CONCLUSION:This preliminary screening of fungal endophytes revealed their potential to yield potent bioactive compounds for drug discovery programmes. 展开更多
关键词 生物工程学 仿生学 基因工程 酶工程
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