Identification and Antibacterial Activity Study of a Terrestrial Strain of Brevibacterium aureus 431

This study isolated and purified strain 431 from an animal probiotic product.Through staining and microscopic examination,colony morphology analysis,biochemical reaction tests,and 16S rDNA sequence alignment,the strain was identified and named Brevibacterium aureus 431.The study focused on the production of biosurfactants by strain 431,and antibacterial activity tests were conducted on the strain and its secondary metabolites.The results showed that strain 431 exhibited no resistance to 10 commonly used drugs,and its concentrated secondary metabolites were highly sensitive to the indicator bacterium Escherichia coli.Oral administration of strain 431 to BALB/c mice resulted in normal mental state,diet,and bowel movements,with no signs of illness or death,indicating that strain 431 is highly safe and non-pathogenic to mice.The study suggests that Brevibacterium aureus 431 has significant research value as a new source of actinomycetes and that its secondary metabolites have potential application value in the development of antibacterial drugs.

Hybrid Teaching Reform of Veterinary Microbiology Based on the Combination of Virtual Simulation and Problem-Based Learning

The Veterinary Microbiology course is centered around the diagnosis and testing of pathogenic microorganisms,with the core value of“moral education and character development.”It reconstructs multidimensional teaching resources by integrating disciplinary achievements with clinical cases and implements a hybrid teaching approach combining virtual simulation and problem-based learning(PBL)through the“three stages+four models+three reflections”framework.Dual-qualification teachers employ various teaching methods,create a“six-in-one”model for ideological and political education,and conduct formative assessments based on the principles of diversified objectives and process emphasis.The hybrid teaching reform addresses issues such as fragmented knowledge,insufficient class hours,weak animal disease diagnostic abilities among students,limited application and expansion of knowledge points,and students’lack of proactive critical thinking skills.The application of hybrid teaching has shown significant advantages and effectiveness,providing a reference for teaching reform in similar microbiology courses.

Comparison of Antibacterial Activities of Six Bacillus amyloliquefaciens Strains

In this study, six bacterial strains were isolated from the sediment, probiotic fermentation products, and lake sediments, they were identified as Bacillus amyloliquefaciens using genetic evolution analysis, which were named B3, B4, B5, XD3, YF6, and YF8. The comparison of the antibacterial activity, hemolytic activity, and antibiotic sensitivity of six Bacillus amyloliquefaciens strains laid a foundation for the development and application of antimicrobial peptide products. A surface activity assay was used to determine the production of biosurfactants in six Bacillus amyloliquefaciens strains. With Staphylococcus aureus and Escherichia coli as indicator bacteria, their antibacterial activity was determined using the agar diffusion method;the same diffusion method was used to determine the antibiotic susceptibility of Bacillus amyloliquefaciens. The results showed that the six Bacillus amyloliquefaciens strains had obvious biosurface activity, and the bacteria inhibited Staphylococcus aureus and Escherichia coli, from strong to weak: YF8, XD3, B3, B4, YF6, and B5. Strain YF8 had the best broad-spectrum bacteriostatic effect, followed by strain XD3. All Bacillus amyloliquefaciens strains were susceptible to 16 common drugs, except for Bacillus amyloliquefaciens strain YF8, which was intermediate to neomycin. The study shows that Bacillus amyloliquefaciens and secondary metabolites have the ability to produce a variety of active peptides, exert a certain inhibitory effect on common pathogens, and have the potential to develop as animal probiotics.

Bioengineering Downstream Technology Online Open Course Drives SPOC Hybrid Teaching Reform Based on Ideological and Political Education

In the context of emerging engineering disciplines,a hybrid teaching reform for the Bioengineering Downstream Technology course,based on ideological and political education and online open courses,is being carried out.This reform focuses on aspects such as“building a professional teacher team for ideological and political education,scientifically designing the ideological and political teaching system,innovating classroom teaching methods,and improving both formative and summative evaluation systems.”The“Craftsmanship in Education and Cultivating Soul and Roots”small private online course hybrid teaching reform for the Bioengineering Downstream Technology online open course provides a replicable model for the comprehensive implementation of ideological and political education in engineering courses and offers a reference for advancing ideological and political education and hybrid teaching reform in new engineering disciplines.

Isolation and Experimental Study on Bacteriostasis of Bacillus haynesii

This experiment aims to isolate and inhibit three bacteria strains to provide candidate strains for the development and application of probiotics.Using bacterial morphological identification,16S rDNA sequence alignment,and genetic evolution analysis,three strains were identified as Bacillus haynesii,named HP01,HD02,and HK03.Through biosurfactant activity tests,C-TAB tests,hemolysis tests,and antibacterial activity analyses,the results showed that all three strains of B.haynesii exhibited significant biosurfactant activity.Additionally,the solutions of the three strains demonstrated a pronounced antibacterial effect on Staphylococcus aureus.The resistance and safety of commonly used drugs were evaluated using the tablet diffusion method and a mouse feeding test.The results indicated that the three strains were not resistant to commonly used antibacterial drugs,and the oral bacterial solution was not pathogenic and had high safety in mice.The study concluded that all three B.haynesii strains met the basic conditions for use,with B.haynesii HP01 being the most promising candidate.

Exploration of Ideological and Political Education in Veterinary Microbiology Experimental Technology

The veterinary microbiology experimental course plays a key role in education and practice,integrating“knowledge,skills,and qualities”into the talent cultivation program.The syllabus has been revised to emphasize the course’s ideological and political education objectives.The extraction of ideological and political elements focuses on developing a curriculum that includes ideological and political teaching materials and cases,ensuring coherence across classroom teaching,social practice,and online courses.By organically integrating ideological and political elements within stories,along with professional knowledge,operational skills,and social practice,the course aims to enhance the appeal and effectiveness of ideological and political education in the experimental course.

Teaching Reform of Veterinary Microbiology Based on the Hybrid Teaching of Ideology and Politics

The veterinary microbiology teaching team thoroughly implements the national education policy,and iteratively promotes the implementation of the teaching reform of Sanquan education.The teaching team is focused on a“student-centered”approach to education,emphasizing the foundation of“cultivating people and nurturing the root and soul of the nation.”By integrating“knowledge exploration,skill training,and value shaping”into the talent training plan,they are reshaping curriculum objectives and emphasizing ideological education in the syllabus.The teaching content system is being reconstructed,combining multiple interactions of blended teaching with the“six-in-one”ideological education model,to achieve the educational goals of the new agricultural“in-class-extracurricular”approach.This effort provides an empirical basis for the ideological and political teaching reform of the veterinary microbiology curriculum.

Research on the Strategic Path of Veterinary Medicine Serving Rural Revitalization in Agriculture-Related Universities

Local agriculture-related universities must have certain regional advantages,advantages of agricultural characteristics,human resources,and science and technology in the process of implementing the rural revitalization strategy.Using literature research and investigation methods,combined with the experience of colleges and universities,the veterinary discipline of rural science and technology correspondent and technology yard,this study discusses our veterinary discipline in education teaching practice and talent training mode innovation,improving the quality of talent training and study on the strategic path of united front service for rural revitalization.

Discussion and Practice on Talent Cultivation Model Based on OBE Concept in Veterinary Medicine Graduate Education

To address the challenges in training veterinary master’s students,it is essential to align with the core objectives of veterinary graduate education.This involves focusing on faculty development,course design,innovative teaching methods,university-industry collaboration,and reforming the evaluation system.By applying the outcome-based education model,new approaches to veterinary graduate training can be developed,particularly for applied talent development in agricultural universities serving local communities.This innovative model emphasizes ideological and political education,aiming to enhance students’abilities in knowledge and practical innovation,preparing them to be well-rounded professionals.The goal is to cultivate senior veterinary experts who are not only skilled in academic research and scientific inquiry but also committed to advancing agriculture,rural areas,and supporting rural revitalization.This approach provides a valuable framework for fostering highly qualified graduates who are both technically proficient and socially responsible.