Unique genes carried by abundant species enhance CH4 emissions during the growing season at the Tibetan Plateau

CH4 emission rates followed an increased pattern during the growing season at Tibetan Plateau.•Unique genes carried by abundant species were positively correlated with CH4 emission rates.•Climate factors influenced CH4 emission rates by regulating microbial community and their genes.Microorganisms play pivotal roles in soil methane(CH4)emissions and their functional genes are origins of a key mechanism for soil CH4-cycling.However,understanding of the roles of specific genes(e.g.,unique or shared genes carried by species)underlying CH4-cycling remains elusive.Here,we measured CH4 emission rates and investigated variations in microbial community and the abundance of genes carried by species during the growing season in alpine meadow on the Tibetan Plateau.We discovered that CH4 emission rates increased from 394.4,745.9,and 1092.7µg CH4 m−2 h−1,in April,June,and August,respectively,and had a positive correlation with unique genes carried by abundant species during the growing season.Moreover,we found that unique genes carried by abundant species involved in methanogenesis processes have a higher abundance than methanotrophic processes.Further analysis indicated that climate factors(i.e.,mean monthly temperature(MMT)and mean monthly precipitation(MMP))influenced microbial community and their functional genes,and therefore affected the CH4 emission rates.Overall,the present study provides a novel insight into the variation of soil CH4 emissions from a functional gene perspective,highlighting the important roles of unique genes carried by abundant species in CH4 emissions in the Tibetan Plateau under seasonal variation.

水面舰艇军人家庭弹性评估量表的编制

目的:编制适用于水面舰艇军人的家庭弹性评估工具。方法:对4654名水面舰艇军人施测,采用探索性因素分析、验证性因素分析、校标效度、信度分析等方法对数据进行分析。结果:探索性因素分析提取7个因子,累计方差解释率为63.923%。验证性因素分析显示,因子模型拟合良好(χ^(2)/Df=4.214,RMSEA=0.031,CFI=0.929,NFI=0.909,GFI=0.915,AGFI=0.905),量表总分与各维度达到中等程度相关(r=0.505~0.688,P<0.01),各维度之间为低度相关(r=0.190~0.310,P<0.01),总量表Cronbach’sα系数为0.904,7个分量表Cronbach’sα系数为0.775~0.864。校标关联效度表明,量表总分与家庭亲密度与适应性量表和心理弹性量表呈显著正相关(r1=0.681,r2=0.637,均P<0.01)。结论:本研究编制的水面舰艇军人家庭弹性评估量表具有良好的信效度,可以作为评估水面舰艇军人家庭弹性的有效工具。