Dear Editor,Harnessing genetic diversity and the introduction of elite alleles from wild relatives or landraces into commercial cultivars has been a major goal in crop breeding programs.Precise modification of the pla...Dear Editor,Harnessing genetic diversity and the introduction of elite alleles from wild relatives or landraces into commercial cultivars has been a major goal in crop breeding programs.Precise modification of the plant genome through clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated protein(Cas)(CRISPR/Cas)-mediated homology-directed repair(HDR)offers a great promise to introduce elite alleles from wild relatives or landraces into commercialized cultivars in the short term.展开更多
Precision genome editing through homology-directed repair(HDR)in plants remains very challenging due to the innately low occurrence of HDR and obstacles in the delivery of sufficient donor repair template(DRT)into pla...Precision genome editing through homology-directed repair(HDR)in plants remains very challenging due to the innately low occurrence of HDR and obstacles in the delivery of sufficient donor repair template(DRT)into plant cells.For single-base substitution,cytidine or adenine base editors have been used successfully in crop plants in recent years.However,base editing is constrained by the distance between the targeted base and PAM motif and cannot install predefined transversion mutations or insertions and deletions(indels).Here,we briefly summarize the strategies reported so far for precision genome editing in plants.Based on the recently developed prime editing strategy,which enables gene replacement without double-strand breaks(DSBs)or DRT in both human cells and plants,we provide our perspectives on exploiting diverse strategies to achieve precise targeted gene/allele replacement toward crop improvement and resilience for sustainable agricultural development.展开更多
基金the Ministry of Agriculture and Rural Affairs of China(2019ZX08010001-001-007 and 2019ZX08010003-002-003)the Central Non-Profit Fundamental Research Funding supported by the Institute of Crop Sciences,Chinese Academy of Agricultural Sciences(S2018QY05)to L.X.No conflict of interest declared.
文摘Dear Editor,Harnessing genetic diversity and the introduction of elite alleles from wild relatives or landraces into commercial cultivars has been a major goal in crop breeding programs.Precise modification of the plant genome through clustered regularly interspaced short palindromic repeat(CRISPR)/CRISPR-associated protein(Cas)(CRISPR/Cas)-mediated homology-directed repair(HDR)offers a great promise to introduce elite alleles from wild relatives or landraces into commercialized cultivars in the short term.
基金Work in the authors'laboratory was supported by the Ministry of Agriculture and Rural Affairs of China(2019ZX08010003-002-003 and 2019ZX08010001-001-007)Central Public-interest Scientific Institution Based Research Fund(S2018QY05,Y2020GH04)National Engineering Laboratory of Crop Molecular Breeding to L.X.
文摘Precision genome editing through homology-directed repair(HDR)in plants remains very challenging due to the innately low occurrence of HDR and obstacles in the delivery of sufficient donor repair template(DRT)into plant cells.For single-base substitution,cytidine or adenine base editors have been used successfully in crop plants in recent years.However,base editing is constrained by the distance between the targeted base and PAM motif and cannot install predefined transversion mutations or insertions and deletions(indels).Here,we briefly summarize the strategies reported so far for precision genome editing in plants.Based on the recently developed prime editing strategy,which enables gene replacement without double-strand breaks(DSBs)or DRT in both human cells and plants,we provide our perspectives on exploiting diverse strategies to achieve precise targeted gene/allele replacement toward crop improvement and resilience for sustainable agricultural development.
