In this paper, we are concerned with the following class of elliptic problems:where 2 = 2N/（N-2） is the critical Sobolev exponent, 2 【 q 【 2 , 0≤μ 【 μˉ=（N-2）2<sub>4</sub> , a（x）, k（x） ∈ C（...In this paper, we are concerned with the following class of elliptic problems:where 2 = 2N/（N-2） is the critical Sobolev exponent, 2 【 q 【 2 , 0≤μ 【 μˉ=（N-2）2<sub>4</sub> , a（x）, k（x） ∈ C（RN ）. Through a compactness analysis of the functional corresponding to the problems , we obtain the existence of positive solutions for this problem under certain assumptions on a（x） and k（x）.展开更多
OBJECTIVE: To evaluate the anti-tumor activity of ethyl acetate fraction(EFA), extracted with ethanol from the root of "Dai-Bai-Jie" in A549 cancer cells and its underlying mechanism.METHODS: "Dai-Bai-J...OBJECTIVE: To evaluate the anti-tumor activity of ethyl acetate fraction(EFA), extracted with ethanol from the root of "Dai-Bai-Jie" in A549 cancer cells and its underlying mechanism.METHODS: "Dai-Bai-Jie" was extracted with 95%ethanol-aqueous(DBJ-1), 50% ethanol-aqueous(DBJ-2), and water(DBJ-3) by reflux method. 95%ethanol-aqueous extract was separated byethyl acetate(EFA) and n-butyl alcohol(DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related pro-teins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity in vivo.RESULTS: DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC5025 and 3.5 μg/mL, respectively. EFA can exhibit the proliferation,arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells in vitro. The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.CONCLUSION: EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53,Caspase-3, and Caspase-8 proteins.展开更多
基金supported by National Natural Science Foundation of China (Grant Nos.10631030, 10871075)the PhD Specialized Grant of the Ministry of Education of China (Grant No. 20060511001)the Natural Science Foundation of Guangdong Province, China (Grant No. 9451064201003736)
文摘In this paper, we are concerned with the following class of elliptic problems:where 2 = 2N/（N-2） is the critical Sobolev exponent, 2 【 q 【 2 , 0≤μ 【 μˉ=（N-2）2<sub>4</sub> , a（x）, k（x） ∈ C（RN ）. Through a compactness analysis of the functional corresponding to the problems , we obtain the existence of positive solutions for this problem under certain assumptions on a（x） and k（x）.
基金Supported by the Grant from the Basic Scientific Research Special of the Central Public Welfare Research Institutes of IMPLADChinese Academy of Medical Sciences:Study on anti-lung cancer chemical constituents and mechanism of"Dai-Bai-Jie"(No.YZYN1501)Study on the resources of"Dai-Bai-Jie"(No.YZYN-10-02)
文摘OBJECTIVE: To evaluate the anti-tumor activity of ethyl acetate fraction(EFA), extracted with ethanol from the root of "Dai-Bai-Jie" in A549 cancer cells and its underlying mechanism.METHODS: "Dai-Bai-Jie" was extracted with 95%ethanol-aqueous(DBJ-1), 50% ethanol-aqueous(DBJ-2), and water(DBJ-3) by reflux method. 95%ethanol-aqueous extract was separated byethyl acetate(EFA) and n-butyl alcohol(DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related pro-teins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity in vivo.RESULTS: DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC5025 and 3.5 μg/mL, respectively. EFA can exhibit the proliferation,arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells in vitro. The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.CONCLUSION: EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53,Caspase-3, and Caspase-8 proteins.
