Preventive effect of TCH-863 on D-Gal induced rat liver damage was further studied with histochemical and electron microscopic cytochemica! techniques. The quantitative analyses on the hirtochemical results were made ...Preventive effect of TCH-863 on D-Gal induced rat liver damage was further studied with histochemical and electron microscopic cytochemica! techniques. The quantitative analyses on the hirtochemical results were made with 4 different image analysers. Experimental procedure: 1) Wistar rats were divided into 4 groups as in Report (l); 2) Routine histochemistry and electron microscopic cytochemistry were carried out; 3) Contents of DNA and RNA in hepatocytes were determined with Quantimet 970 image analyser.展开更多
Cordyceps sinensis (CS) is well known as an ancient Chinese herb. It is used to expand bronchial smooth muscles, inhibit tumor growth and decrease blood pressure. Cordyceps sinensis is composed of two parts. One is th...Cordyceps sinensis (CS) is well known as an ancient Chinese herb. It is used to expand bronchial smooth muscles, inhibit tumor growth and decrease blood pressure. Cordyceps sinensis is composed of two parts. One is the dead larvae body; the other is the stroma like withered grass. Up to now, few genome database articles about Cordyceps sinensis have been reported. In this study, two cDNA libraries were constructed using the worm part and grass part respectively for the first time. 12192 and 15456 clones from the worm-part (CSCA) and grass-part (CSBA) library were respectively picked. Sequences derived from CSCA were clustered into 1333 contigs and 2469 singlets, while those from CSBA were clustered into 1297 contigs and 2875 singlets. These ESTs include sequences representing a significant portion of proteins encoding genes in cell signalling, metabolism, information storage and processing. Some enzymes encoding genes were also found and linked with CS’s physiology such as proteases, peptidases, lipases and chitinase. Pairwise comparison between the two cDNA libraries was also studied. Some ESTs were found only in CSCA and some only in CSBA. Finally, a comparative genomics research was performed with Fusarium graminearum, Aspergillus nidulans, Neurospora crassa and Saccharomyces bayanus. The results indicate that the fungus’ genes maybe have complicated variation at the nucleic acid level, but the proteins translated are still conservative.展开更多
文摘Preventive effect of TCH-863 on D-Gal induced rat liver damage was further studied with histochemical and electron microscopic cytochemica! techniques. The quantitative analyses on the hirtochemical results were made with 4 different image analysers. Experimental procedure: 1) Wistar rats were divided into 4 groups as in Report (l); 2) Routine histochemistry and electron microscopic cytochemistry were carried out; 3) Contents of DNA and RNA in hepatocytes were determined with Quantimet 970 image analyser.
基金supported by Beijing Institute of Genomics (CAS) and Beijing University of Chinese Medicine
文摘Cordyceps sinensis (CS) is well known as an ancient Chinese herb. It is used to expand bronchial smooth muscles, inhibit tumor growth and decrease blood pressure. Cordyceps sinensis is composed of two parts. One is the dead larvae body; the other is the stroma like withered grass. Up to now, few genome database articles about Cordyceps sinensis have been reported. In this study, two cDNA libraries were constructed using the worm part and grass part respectively for the first time. 12192 and 15456 clones from the worm-part (CSCA) and grass-part (CSBA) library were respectively picked. Sequences derived from CSCA were clustered into 1333 contigs and 2469 singlets, while those from CSBA were clustered into 1297 contigs and 2875 singlets. These ESTs include sequences representing a significant portion of proteins encoding genes in cell signalling, metabolism, information storage and processing. Some enzymes encoding genes were also found and linked with CS’s physiology such as proteases, peptidases, lipases and chitinase. Pairwise comparison between the two cDNA libraries was also studied. Some ESTs were found only in CSCA and some only in CSBA. Finally, a comparative genomics research was performed with Fusarium graminearum, Aspergillus nidulans, Neurospora crassa and Saccharomyces bayanus. The results indicate that the fungus’ genes maybe have complicated variation at the nucleic acid level, but the proteins translated are still conservative.
