BACKGROUND Loss and/or dysfunction of interstitial Cajal-like cells(ICLCs)in the gallbladder may promote cholesterol gallstone formation by decreasing gallbladder motility.AIM To study the effect of cholesterol on the...BACKGROUND Loss and/or dysfunction of interstitial Cajal-like cells(ICLCs)in the gallbladder may promote cholesterol gallstone formation by decreasing gallbladder motility.AIM To study the effect of cholesterol on the proliferation and apoptosis of ICLCs from guinea pig gallbladders.METHODS Guinea pig gallbladder ICLCs were isolated and cultured in vitro.The cells were exposed to cholesterol solutions at different concentrations(0,25,50,and 100 mg/L)for 24 h.Then,cell proliferation was detected by the CCK-8 method and the apoptosis rate was detected by flow cytometry.Further,the expression of the c-Kit protein was detected by Western blot and the expression level of c-Kit m RNA in the cells was detected by real-time quantitative PCR.RESULTS After ICLCs were cultured with cholesterol at concentrations of 25,50,and 100 mg/L,the proliferation rates decreased significantly(P<0.05),whereas the apoptosis rates increased significantly(P<0.05).Moreover,the expression of cKit protein and m RNA decreased significantly(P<0.05).CONCLUSION High cholesterol concentrations can inhibit the proliferation of ICLCs and promote apoptosis.This decrease in the ICLC proliferation rate might be caused by the inhibition of the stem cell factor/c-Kit signaling pathway.展开更多
基金Supported by the National Natural Science Foundation of China,No.81000183the Natural Science Foundation of Liaoning Province,No.20180550125。
文摘BACKGROUND Loss and/or dysfunction of interstitial Cajal-like cells(ICLCs)in the gallbladder may promote cholesterol gallstone formation by decreasing gallbladder motility.AIM To study the effect of cholesterol on the proliferation and apoptosis of ICLCs from guinea pig gallbladders.METHODS Guinea pig gallbladder ICLCs were isolated and cultured in vitro.The cells were exposed to cholesterol solutions at different concentrations(0,25,50,and 100 mg/L)for 24 h.Then,cell proliferation was detected by the CCK-8 method and the apoptosis rate was detected by flow cytometry.Further,the expression of the c-Kit protein was detected by Western blot and the expression level of c-Kit m RNA in the cells was detected by real-time quantitative PCR.RESULTS After ICLCs were cultured with cholesterol at concentrations of 25,50,and 100 mg/L,the proliferation rates decreased significantly(P<0.05),whereas the apoptosis rates increased significantly(P<0.05).Moreover,the expression of cKit protein and m RNA decreased significantly(P<0.05).CONCLUSION High cholesterol concentrations can inhibit the proliferation of ICLCs and promote apoptosis.This decrease in the ICLC proliferation rate might be caused by the inhibition of the stem cell factor/c-Kit signaling pathway.