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MicroRNA-486和TGF-β_1对人主动脉瓣膜钙化的影响及其机制研究 被引量:1
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作者 蒋伟坚 方明 +3 位作者 苏锦文 王温慧 王雪君 李新明 《中国现代医学杂志》 CAS 2018年第32期25-32,共8页
目的研究microRNA-486(miRNA-486)对人主动脉瓣膜间质细胞(VICs)钙化的影响及作用机制。方法体外培养VICs,经成骨诱导后用双荧光素报告基因检测miRNA-486靶基因。转染miR-486mimic、miR-486 Inhibitors后,将细胞分为miR-486 mimic组、mi... 目的研究microRNA-486(miRNA-486)对人主动脉瓣膜间质细胞(VICs)钙化的影响及作用机制。方法体外培养VICs,经成骨诱导后用双荧光素报告基因检测miRNA-486靶基因。转染miR-486mimic、miR-486 Inhibitors后,将细胞分为miR-486 mimic组、miR-486 mimic NC组、miR-486 Inhibitors组、miR-486 Inhibitors NC组。采用茜红素染色、ALP试验分析各组钙化程度,实时荧光定量聚合酶链反应(qRT-PCR)、免疫荧光试验检测miR-486靶基因及成骨相关因子mRNA和蛋白的表达水平。结果双荧光素报告基因检测和Western blot检测结果显示,转录生长因子-β_1(TGF-β_1)为潜在靶基因。茜红素染色、ALP试验结果显示,miR-486 mimic组钙化程度高于miR-486 Inhibitors组。qRT-PCR、免疫荧光试验结果表明,miR-486 mimic组TGF-β_1、SMAD3 mRNA和蛋白表达水平较低(P<0.05);miR-486 mimic组RUNX2、骨钙素mRNA表达水平较miR-486 Inhibitors组高(P<0.05)。结论 miR-486可通过下调TGF-β_1的表达,促进RUNX2、骨钙素的表达和活性,诱导间质细胞向成骨细胞分化。 展开更多
关键词 心脏瓣膜疾病 microRNA-486 转录生长因子-β1 茜红素染色 碱性磷酸酶活性 主动脉瓣膜
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Left atrial appendage occlusion in a mirror-image dextrocardia:A case report and review of literature 被引量:2
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作者 Bei Tian Chuang Ma +4 位作者 jin-wen su Jun Luo Hong-Xia sun Jie su Zhong-Ping Ning 《World Journal of Clinical Cases》 SCIE 2022年第4期1357-1365,共9页
BACKGROUND In mirror-image dextrocardia,the anterior-posterior position of the cardiac chambers and great vessels is maintained,but the left-right orientation of the abdominal organs is reversed.The abnormal anatomy o... BACKGROUND In mirror-image dextrocardia,the anterior-posterior position of the cardiac chambers and great vessels is maintained,but the left-right orientation of the abdominal organs is reversed.The abnormal anatomy of the heart poses surgical challenges and problems in dealing with surgical risk and monitoring complications.There are few reports on closure of the left atrial appendage(LAA)in dextrocardia and no reports on the application of enhanced recovery after surgery(ERAS)following LAA occlusion(LAAO)procedures.CASE SUMMARY The objective for this case was to ensure perioperative safety and accelerate postoperative recovery from LAAO in a patient with mirror-image dextrocardia.ERAS was guided by the theory and practice of nursing care.Atrial fibrillation was diagnosed in a 77-year-old male patient,in whom LAAO was performed.The 2019 guidelines for perioperative care after cardiac surgery recommend that the clinical nursing procedures for patients with LAAO should be optimized to reduce the incidence of perioperative complications and ensure patient safety.Music therapy can be used throughout perioperative treatment and nursing to improve the anxiety symptoms of patients.CONCLUSION The procedure was uneventful and proceeded without complications.Anxiety symptoms were improved. 展开更多
关键词 Atrial fibrillation DEXTROCARDIA Left atrial appendage occlusion Music therapy Enhanced recovery after surgery Case report
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Mesenchymal-epithelial Transition Factor Regulates Monocyte Function during Mycobacterial Infection via Indoleamine 2,3-dioxygenase 被引量:1
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作者 Bing-fen YANG Fei ZHAI +6 位作者 Hong-juan AN Jing JIANG Zhi-hong CAO Yan-hua LIU jin-wen su Ruo WANG Xiao-xing CHENG 《Current Medical Science》 SCIE CAS 2022年第2期407-416,共10页
Objective Mycobacterium tuberculosis(Mtb),the causative agent of tuberculosis(TB),causes an estimated 1.6 million human deaths annually,but the pathogenesis of TB remains unclear.Immunity plays a critical role in the ... Objective Mycobacterium tuberculosis(Mtb),the causative agent of tuberculosis(TB),causes an estimated 1.6 million human deaths annually,but the pathogenesis of TB remains unclear.Immunity plays a critical role in the onset and outcome of TB.This study aimed to uncover the roles of innate and adaptive immunity in TB.Methods The gene expression profiles generated by RNA sequencing from human peripheral blood mononuclear cells(PBMCs)stimulated with or without Mtb strain H37Rv antigens were analyzed.A total of 973 differentially expressed mRNAs were identified.Results The differentially expressed genes were enriched in innate immunity signaling functions.The mesenchymal-epithelial transition factor(MET)gene was significantly upregulated in CD14^(+)monocytes.A MET inhibitor improved the uptake of the BCG strain by monocytes and macrophages as well as inhibited the expression of indoleamine 2,3-dioxygenase(IDO).The expression of IDO was increased in PBMCs stimulated with Mtb antigens,and the IDO inhibitor promoted the expression of CD40,CD83,and CD86.Conclusion Our results might provide clues regarding the immunomodulatory mechanisms used by Mtb to evade the host defense system. 展开更多
关键词 MONOCYTES MYCOBACTERIA mesenchymal-epithelial transition factor indoleamine 2 3-dioxygenase
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