An undescribed pyrrole acid,1-(4′-methoxy-4′-oxobutyl)-1 H-pyrrole-2,5-dicarboxylic acid(1)and one known pyr-role acid(2)were isolated from the fruits of Phyllanthus emblica.The structures of these compounds were el...An undescribed pyrrole acid,1-(4′-methoxy-4′-oxobutyl)-1 H-pyrrole-2,5-dicarboxylic acid(1)and one known pyr-role acid(2)were isolated from the fruits of Phyllanthus emblica.The structures of these compounds were elucidated via the comprehensive analyses of IR,HRESIMS,1D and 2D spectroscopic data.A series of biological assays revealed that compounds 1 and 2 could inhibit LPS-induced over-production of nitric oxide(NO),interleukin-6(IL-6),monocyte chemotactic protein 1(MCP-1)and tumor necrosis factor-α(TNF-α)by reducing the phosphorylation of extracellular regulated protein kinases(ERK)and c-Jun N-terminal kinases(JNK)in RAW 264.7 cells.Additionally,compounds 1 and 2 were found to reduce lipid deposition and increase the mRNA expression of ATP-binding cassette transporter A1 in oxidized low-density lipoprotein-treated RAW264.7 macrophages.展开更多
Heat stress is a serious and widespread threat to the quality and yield of many crop species,including grape(Vitis vinifera L.),which is cultivated worldwide.Here,we conducted phosphoproteomic and acetylproteomic anal...Heat stress is a serious and widespread threat to the quality and yield of many crop species,including grape(Vitis vinifera L.),which is cultivated worldwide.Here,we conducted phosphoproteomic and acetylproteomic analyses of leaves of grape plants cultivated under four distinct temperature regimes.The phosphorylation or acetylation of a total of 1011 phosphoproteins with 1828 phosphosites and 96 acetyl proteins with 148 acetyl sites changed when plants were grown at 35℃,40℃,and 45℃in comparison with the proteome profiles of plants grown at 25℃.The greatest number of changes was observed at the relatively high temperatures.Functional classification and enrichment analysis indicated that phosphorylation,rather than acetylation,of serine/arginine-rich splicing factors was involved in the response to high temperatures.This finding is congruent with previous observations by which alternative splicing events occurred more frequently in grapevine under high temperature.Changes in acetylation patterns were more common than changes in phosphorylation patterns in photosynthesis-related proteins at high temperatures,while heat-shock proteins were associated more with modifications involving phosphorylation than with those involving acetylation.Nineteen proteins were identified with changes associated with both phosphorylation and acetylation,which is consistent with crosstalk between these posttranslational modification types.展开更多
基金Scientific and technological innovation project of China Academy of Chinese Medical Sciences(CI2021A04409,CI2021A04404,CI2021A04405)the fundamental research funds for the central public welfare research institutes(No.ZZ13-YQ-061,ZXKT22012,ZXKT22039).
文摘An undescribed pyrrole acid,1-(4′-methoxy-4′-oxobutyl)-1 H-pyrrole-2,5-dicarboxylic acid(1)and one known pyr-role acid(2)were isolated from the fruits of Phyllanthus emblica.The structures of these compounds were elucidated via the comprehensive analyses of IR,HRESIMS,1D and 2D spectroscopic data.A series of biological assays revealed that compounds 1 and 2 could inhibit LPS-induced over-production of nitric oxide(NO),interleukin-6(IL-6),monocyte chemotactic protein 1(MCP-1)and tumor necrosis factor-α(TNF-α)by reducing the phosphorylation of extracellular regulated protein kinases(ERK)and c-Jun N-terminal kinases(JNK)in RAW 264.7 cells.Additionally,compounds 1 and 2 were found to reduce lipid deposition and increase the mRNA expression of ATP-binding cassette transporter A1 in oxidized low-density lipoprotein-treated RAW264.7 macrophages.
基金supported by the National Key Research and Development Program of China(2018YFD1000300)the National Natural Science Foundation of China(Grant no.31672120).
文摘Heat stress is a serious and widespread threat to the quality and yield of many crop species,including grape(Vitis vinifera L.),which is cultivated worldwide.Here,we conducted phosphoproteomic and acetylproteomic analyses of leaves of grape plants cultivated under four distinct temperature regimes.The phosphorylation or acetylation of a total of 1011 phosphoproteins with 1828 phosphosites and 96 acetyl proteins with 148 acetyl sites changed when plants were grown at 35℃,40℃,and 45℃in comparison with the proteome profiles of plants grown at 25℃.The greatest number of changes was observed at the relatively high temperatures.Functional classification and enrichment analysis indicated that phosphorylation,rather than acetylation,of serine/arginine-rich splicing factors was involved in the response to high temperatures.This finding is congruent with previous observations by which alternative splicing events occurred more frequently in grapevine under high temperature.Changes in acetylation patterns were more common than changes in phosphorylation patterns in photosynthesis-related proteins at high temperatures,while heat-shock proteins were associated more with modifications involving phosphorylation than with those involving acetylation.Nineteen proteins were identified with changes associated with both phosphorylation and acetylation,which is consistent with crosstalk between these posttranslational modification types.
