A series of double-hydrophilic double-grafted PMA-g-PEG/PDMA copolymers, which contained poly（methacrylate） （PMA） as backbone, poly（ethylene glycol） （PEG） and poly（N,N-dimethylacrylamide） （PDMA） as side ch...A series of double-hydrophilic double-grafted PMA-g-PEG/PDMA copolymers, which contained poly（methacrylate） （PMA） as backbone, poly（ethylene glycol） （PEG） and poly（N,N-dimethylacrylamide） （PDMA） as side chains synthesized successfully by using reversible addition-fragmentation chain transfer （RAFT） polymerization and atom transfer radical polymerization （ATRP）, were used as physical coatings for the evaluation of protein-resistant properties by capillary electrophoresis （CE）. Electroosmotic flow （EOF） measurement results showed that the PMA-g-PEG/PDMA copolymer coated capillaries could suppress electroosmotic mobility in a wide pH range （pH = 2.8-9.8） and EOF mobility decreased with the increase of copolymer molecular mass and PDMA content. At the same time, protein recovery, theoretical plate number of separation and repeatability of migration time demonstrated that antifouling efficiency was improved with the increase of molecular mass and PEG content.展开更多
基金supported by the National Natural Science Foundation of China (No. 21074124)Ministry of Science and Technology of China (No. 2012CB933802)
文摘A series of double-hydrophilic double-grafted PMA-g-PEG/PDMA copolymers, which contained poly（methacrylate） （PMA） as backbone, poly（ethylene glycol） （PEG） and poly（N,N-dimethylacrylamide） （PDMA） as side chains synthesized successfully by using reversible addition-fragmentation chain transfer （RAFT） polymerization and atom transfer radical polymerization （ATRP）, were used as physical coatings for the evaluation of protein-resistant properties by capillary electrophoresis （CE）. Electroosmotic flow （EOF） measurement results showed that the PMA-g-PEG/PDMA copolymer coated capillaries could suppress electroosmotic mobility in a wide pH range （pH = 2.8-9.8） and EOF mobility decreased with the increase of copolymer molecular mass and PDMA content. At the same time, protein recovery, theoretical plate number of separation and repeatability of migration time demonstrated that antifouling efficiency was improved with the increase of molecular mass and PEG content.
