The melon thrips, Thrips palmi Karny, is an economically important pest on many vegetables from Solanaceae and Cucurbitaceae. In this study, we developed novel microsatellite markers for Thrips palmi. First, we obtain...The melon thrips, Thrips palmi Karny, is an economically important pest on many vegetables from Solanaceae and Cucurbitaceae. In this study, we developed novel microsatellite markers for Thrips palmi. First, we obtained randomly sequenced regions from the genome using next-generation sequencing and assembled 356 Mb genomic sequences. In total, 155,789 microsatellites were identified from the genomic sequences, of which, 64.02% were loci of dinucleotide repeat. Sixty primer pairs were initially validated in seven individuals. Thirty-five polymorphic markers were retained according to the amplification efficiency and polymorphism.These 35 microsatellite loci were then assessed in 96 individuals in four geographical populations collected from China. The allele numbers ranged from 2 to 19 with an average value of 6.9 per locus, while the polymorphism information content ranged from 0.2219 to 0.8490.The observed and expected heterozygosity varied from 0.5252 to 0.5367 and 0.5934 to 0.6148,respectively. Genetic structure was found between populations from southern and northern China. These novel microsatellite markers developed in our study provide abundant molecular markers for the genetic study of T. palmi.展开更多
The weevils Eucryptorrhynchus brandti(Harold)and E.scrobiculatus(Motschulsky)are two pests of the tree of heaven,Ailanthus altissima.They usually co-occur on their host tree.However,these two species have a niche sepa...The weevils Eucryptorrhynchus brandti(Harold)and E.scrobiculatus(Motschulsky)are two pests of the tree of heaven,Ailanthus altissima.They usually co-occur on their host tree.However,these two species have a niche separation with different eggs-laying locations and larvae-feeding parts on the tree.Here,we characterized the distribution of microsatellites in the genome and developed genome-wide microsatellite markers shared between these two weevils.A total of 12,524 and 11,030 potential microsatellites were identified for E.brandti and E.scrobiculatus,respectively.Microsatellites with AT,TA,AC,TG,CA repeat motifs were most common for both species.We designed primers universal for two weevils and developed 14 loci with high amplification efficacy and polymorphism.We validated these microsatellite markers in three populations of each species.In E.brandti,the observed and expected heterozygosity for the genotyped loci ranged from 0.17-0.24 and 0.44-0.54;in E.scrobiculatus,the above values ranged from 0.06-0.18 and 0.18-0.27.Based on the developed microsatellite markers,we found that populations of both weevils have a clear pattern of genetic differentiation.Our study provides valuable genetic markers for ecological,genetic,and evolutionary study of these two weevils.展开更多
基金provided jointly by Promotion and Innovation of Beijing Academy of Agriculture and Forestry Sciences(KJCX20180113)the Natural Science Foundation of Beijing Municipality(6184037)Beijing Key Laboratory of Environmentally Friendly Pest Management on Northern Fruits(BZ0432)
文摘The melon thrips, Thrips palmi Karny, is an economically important pest on many vegetables from Solanaceae and Cucurbitaceae. In this study, we developed novel microsatellite markers for Thrips palmi. First, we obtained randomly sequenced regions from the genome using next-generation sequencing and assembled 356 Mb genomic sequences. In total, 155,789 microsatellites were identified from the genomic sequences, of which, 64.02% were loci of dinucleotide repeat. Sixty primer pairs were initially validated in seven individuals. Thirty-five polymorphic markers were retained according to the amplification efficiency and polymorphism.These 35 microsatellite loci were then assessed in 96 individuals in four geographical populations collected from China. The allele numbers ranged from 2 to 19 with an average value of 6.9 per locus, while the polymorphism information content ranged from 0.2219 to 0.8490.The observed and expected heterozygosity varied from 0.5252 to 0.5367 and 0.5934 to 0.6148,respectively. Genetic structure was found between populations from southern and northern China. These novel microsatellite markers developed in our study provide abundant molecular markers for the genetic study of T. palmi.
基金supported by the National Natural Science Foundation of China(31770691)the National Key R&D Program of China(2018YFC1200400)。
文摘The weevils Eucryptorrhynchus brandti(Harold)and E.scrobiculatus(Motschulsky)are two pests of the tree of heaven,Ailanthus altissima.They usually co-occur on their host tree.However,these two species have a niche separation with different eggs-laying locations and larvae-feeding parts on the tree.Here,we characterized the distribution of microsatellites in the genome and developed genome-wide microsatellite markers shared between these two weevils.A total of 12,524 and 11,030 potential microsatellites were identified for E.brandti and E.scrobiculatus,respectively.Microsatellites with AT,TA,AC,TG,CA repeat motifs were most common for both species.We designed primers universal for two weevils and developed 14 loci with high amplification efficacy and polymorphism.We validated these microsatellite markers in three populations of each species.In E.brandti,the observed and expected heterozygosity for the genotyped loci ranged from 0.17-0.24 and 0.44-0.54;in E.scrobiculatus,the above values ranged from 0.06-0.18 and 0.18-0.27.Based on the developed microsatellite markers,we found that populations of both weevils have a clear pattern of genetic differentiation.Our study provides valuable genetic markers for ecological,genetic,and evolutionary study of these two weevils.
