左心室压力-应变环评价缺铁性贫血患者左室收缩功能的研究

目的:应用无创性左室压力应变环技术(LV-PSL)定量评估缺铁性贫血(IDA)患者左室收缩功能的早期改变。方法:选取2022年3月—2023年2月我医院确诊的IDA患者62例作为病例组,按照血红蛋白(Hb)浓度的高低分为两组:B组Hb>9 g/dL,C组Hb>6~9 g/dL。同期33例健康人作为对照A组。收集相关临床资料数据,对三组均进行超声心动图检查并采集常规超声参数,利用二维斑点追踪技术和LV-PSL分析得出左室整体纵向应变(GLS)、整体做功指数(GWI)、整体有用功(GCW)、整体无用功(GWW)、整体做功效率(GWE)。比较三组间GLS及心肌做功参数的变化,分析心肌做功参数与GLS的相关性。结果:与A组比较,B组GWI、GCW均减低(均P<0.01)。与A组、B组比较,C组GLS、GWI、GCW和GWE、E/A均减低,GWW、LVEDV、LVESV、meanE/e’均增加(均P<0.01)。相关性分析结果显示,GLS与GWI、GCW、GWE呈正相关关系(r=0.697、0.681、0.447,均P<0.01),与GWW呈负相关关系(r=-0.411,均P<0.01)。结论:LV-PSL可无创定量评估长期不同程度IDA患者早期左室收缩功能受损情况,且左室收缩功能随着贫血程度的加重逐步降低。

芒硝外敷联合乌司他丁对胰腺炎大鼠肠屏障功能、胰腺细胞活性及PI3K/Akt/mTOR信号的影响

目的探讨芒硝外敷联合乌司他丁对胰腺炎大鼠肠屏障功能、胰腺细胞活性及PI3K/Akt/mTOR信号的影响。方法选取50只SPF级SD雄性大鼠,随机分为正常(N)组,模型(M)组,芒硝外敷(G)组,乌司他丁(U)组,芒硝外敷+乌司他丁(O)组,每组10只,对M组、G组、U组、O组采用开腹经胰胆管逆行性注射5%牛黄胆酸钠法建立胰腺炎模型,N组不建立该模型,建模成功后,对G组腹部给予芒硝外敷,对U组腹腔注射乌司他丁5万U/kg,对O组腹部给予芒硝外敷和腹腔注射乌司他丁5万U/kg,N组、M组同期给予腹腔注射同体积生理盐水,HE法检测胰腺组织病理形态,电镜检测肠屏障功能,TUNEL法检测胰腺细胞凋亡,免疫印迹法检测PI3K/Akt/mTOR信号相关蛋白表达。结果与N组相比,M组可见胰腺组织明显病理变化及肠屏障功能损伤,与M组比较,G组、U组、O组病理形态及肠屏障功能明显改善,且O组改善最为明显;与N组相比,M组大鼠肠粘膜损伤程度、胰腺组织中PI3K、p-Akt、p-mTOR蛋白表达显著升高,胰腺细胞凋亡水平显著降低(均P<0.05),与M组比较,G组、U组、O组大鼠肠粘膜损伤程度、胰腺组织中PI3K、p-Akt、p-mTOR蛋白表达显著降低(均P<0.05),胰腺细胞凋亡水平显著升高(P<0.05),且U组与G组相比差异无统计学意义(P>0.05),O组比U组变化明显(P<0.05)。结论芒硝外敷联合乌司他丁可显著改善胰腺炎大鼠肠屏障功能及胰腺细胞活性,并显著抑制PI3K/Akt/mTOR信号。

基于社会网络分析方法的二氧化碳地质封存风险传染特征研究

二氧化碳(CO_(2))地质封存技术作为深度减排不可或缺的必要手段,对保护生态环境、推动全球中长期应对气候变化的国际合作以及推进社会绿色、循环、低碳发展具有重要意义。文章总结了二氧化碳地质封存项目全生命周期中可能出现的46个风险因素,运用社会网络分析方法构建风险网络关系模型,对二氧化碳地质封存项目风险因素间的传递作用关系进行了研究。通过风险网络整体与局部参数分析确定风险传递过程中的关键起始节点、关键致险节点、关键传递节点等关键因素,识别出三条潜在风险传递链:(1)地质灾害风险→CO_(2)供应、注入或运输意外中断风险→预期外的建设或操作成本变化风险;(2)操作人员知识不足或无相应资质风险→人为泄漏风险→项目对环境的破坏风险→公众参与风险→预期外的建设或操作成本变化风险;(3)部门协调不当风险→人才招聘和管理风险→操作人员知识不足或无相应资质风险→预期外的建设或操作成本变化风险。研究旨在为二氧化碳地质封存项目风险研究提供理论创新与技术参考,进而促进CO_(2)捕集、利用与封存项目的健康发展。

荒漠化治理综合效益评估——以柯柯牙工程为例

荒漠化治理是干旱、半干旱地区生态治理的重要内容。评估荒漠化治理工程的综合效益对沙区生态经济综合效益具有重要意义。本文以柯柯牙工程为例,结合生态学、经济学、土壤学等交叉学科的知识,梳理总结了柯柯牙工程的评估内容,分析了经济效益、生态效益、社会效益适用的评估方法,构建了评估荒漠化治理工程综合效益的评价指标体系,对典型模式的荒漠化治理工程综合效益进行了评价。综合分析结果:(1)结合柯柯牙工程的修复技术和修复机理,构建了一套综合效益评估体系。其中经济效益包括取用材林效益、食用产品效益、节水效益和旅游观光效益;生态效益包括涵养水源效益、固碳释氧效益、净化环境效益、保育土壤效益、生物多样性效益、森林防护效益、林木生产营养物质效益和防风固沙效益;社会效益包括娱乐文化效益、人类身体健康效益和劳动力就业效益。(2)截至2020年,柯柯牙工程综合效益为1.14千亿元。经济效益198.97亿元、生态效益734.11亿元、社会效益205.81亿元,生态效益和社会效益均超过了经济效益。(3)柯柯牙工程综合效益中,保育土壤效益、林木生产营养物质效益、食用产品效益、劳动力就业效益占其83.53%,而森林防护效益、人类身体健康效益、防风固沙效益、生物多样性效益等占16.47%。

粤港澳大湾区CO_(2)排放趋势、驱动因素及减排路径

粤港澳大湾区是我国为建设世界级城市群提出的国家战略,其CO_(2)排放趋势、社会经济驱动因素和减排路径对区域经济高质量发展具有重要意义。本研究编制了2000-2019年粤港澳大湾区CO_(2)排放清单,采用对数平均迪氏指数法探究CO_(2)排放的关键驱动力。结果表明,粤港澳大湾区CO_(2)排放在2017年后明显放缓,已经与国内生产总值(GDP)增长脱钩。经济增长和能源强度分别是驱动和抑制粤港澳大湾区CO_(2)排放增加的主要因素。能源生产和重工业对粤港澳大湾区CO_(2)排放增长的推动作用降低,服务业正成为主要驱动力。粤港澳大湾区通过产业结构调整和升级实现低碳发展取得了显著成效。深圳和香港地区的产业升级以及深圳、广州和佛山的技术进步抑制了粤港澳大湾区CO_(2)排放增长。粤港澳大湾区各城市异质性增加了减排指标分配难度和制定区域碳中和路线图的复杂性。本研究提出了粤港澳大湾区城市分级减排策略和碳达峰碳中和建议,为制定粤港澳大湾区城市碳达峰和碳中和行动方案以及其他城市和地区的低碳发展提供了科学依据。

多维距离对我国矿产资源企业跨国并购成败的影响--地理、经济、制度与文化

以跨国并购的方式获取对海外矿产资源的控制权,有助于保障我国矿产资源安全,推动国内经济高质量发展。基于我国矿产资源企业的跨国并购数据,利用二元Logit模型实证研究多维距离对我国矿产资源企业跨国并购成败的影响。结果表明:地理、制度和文化距离均对我国矿产资源企业跨国并购产生促进效应,且距离差异越大并购越容易成功;经济距离对并购成败的影响不显著。因此,我国矿产资源企业的跨国并购决策应充分考虑多维距离的影响,降低并购风险,又好又快地“走出去”。

骨髓间充质干细胞来源外泌体诱导自噬对MPP+抑制SH-SY5Y细胞存活的影响及其机制

目的:探讨骨髓间充质干细胞(BMSCs)来源外泌体(Exo)诱导自噬对1-甲基-4-苯基吡啶离子(MPP^(+))抑制人神经母细胞瘤SH-SY5Y细胞存活的影响,阐明其作用机制。方法:将人神经细胞肿瘤SH-SY5Y细胞分别给予不同浓度(0、0.25、0.50、1.00和2.00 mmol·L^(-1))MMP+作用24和48 h后检测各组细胞存活率。实验分为对照组[给予磷酸盐缓冲液(PBS)]、MPP^(+)组(给予0.50 mmol·L^(-1) MPP^(+))、MPP^(+)+BMSCs的上清液(BMSCs-Sup)组(给予0.50 mmol·L^(-1) MPP^(+)和BMSCs Sup)、MPP^(+)+BMSCs-Exo组(给予0.50 mmol·L^(-1) MPP^(+)和100 mg·L^(-1) BMSCs Exo)、MPP^(+)+雷帕霉素(Rap)组(给予0.50 mmol·L^(-1) MPP^(+)和2 mmol·L^(-1) Rap)和MPP^(+)+BMSCs-Exo+3-甲基腺嘌呤(3-MA)组(给予0.50 mmol·L^(-1) MPP^(+)、100 mg·L^(-1) BMSCs-Exo和1 mmol·L^(-1)3-MA)。MTT法检测各组SH-SY5Y细胞存活率,共聚焦显微镜下观察吖啶橙染色后各组SH-SY5Y细胞中自噬酸性囊泡荧光强度,Western blotting法检测各组细胞中CD63、TGS101和Calnexin蛋白表达情况。结果:MTT检测,SH-SY5Y细胞存活率随着MPP^(+)浓度的升高或处理时间的延长逐渐降低(P<0.05),且呈浓度和时间依赖性。与MPP^(+)组比较,MPP^(+)+BMSCs-Sup组、MPP^(+)+BMSCs-Exo组和MPP^(+)+Rap组SH-SY5Y细胞存活率升高(P<0.05);与MPP^(+)+BMSCs-Exo组比较,MPP^(+)+BMSCs-Exo+3-MA组SH-SY5Y细胞存活率降低(P<0.05)。细胞吖啶橙染色,与MPP^(+)组比较,MPP^(+)+BMSCs-Sup组、MPP^(+)+BMSCs-Exo组和MPP^(+)+Rap组SH-SY5Y细胞中自噬酸性囊泡荧光强度增加(P<0.05);与MPP^(+)+BMSCs-Exo组比较,MPP^(+)+BMSCs-Exo+3-MA组SH-SY5Y细胞中自噬酸性囊泡荧光强度降低(P<0.05)。Western blotting法检测,各组细胞中外泌体标志蛋白CD63和TGS101蛋白高表达,内质网标志蛋白Calnexin低表达。结论:BMSCs-Exo通过激活细胞自噬抵抗MPP^(+)诱导的SH-SY5Y细胞存活率下降。

褪黑素对过氧化氢诱导人神经母细胞瘤SH-SY5Y细胞氧化应激的改善作用及其机制

目的:研究褪黑素对过氧化氢(H_(2)O_(2))诱导的人神经母细胞瘤细胞(SH-SY5Y细胞)氧化应激损伤的保护作用,并探讨其作用机制。方法:体外培养SH-SY5Y细胞,将SH-SY5Y细胞分为对照组、H_(2)O_(2)组、不同浓度褪黑素组和2-苯基-N-乙酰色胺(Luzindole)组。对照组为正常培养SHSY5Y细胞,H_(2)O_(2)组加入含有200μmol·L^(-1)H_(2)O_(2)的培养基,不同浓度褪黑素组加入不同浓度(1、5和10μmol·L^(-1))褪黑素和含有200μmol·L^(-1)H_(2)O_(2)的培养基,Luzindole组加入50μmol·L^(-1)Luzindole、10μmol·L^(-1)褪黑素和含有200μmol·L^(-1)H_(2)O_(2)的培养基。采用CCK-8法检测各组SHSY5Y细胞存活率,流式细胞术检测各组细胞凋亡率,DCFH-DA荧光探针检测各组细胞中活性氧(ROS)水平,Western blotting法检测各组细胞中微管相关蛋白轻链3-Ⅱ(LC3-Ⅱ)蛋白表达水平,荧光显微镜观察各组自噬泡荧光强度。结果:与对照组比较,H_(2)O_(2)组SH-SY5Y细胞存活率降低(P<0.01),ROS水平和细胞凋亡率升高(P<0.01)。与H_(2)O_(2)组比较,不同浓度褪黑素组SH-SY5Y细胞存活率升高(P<0.05),ROS水平和细胞凋亡率降低(P<0.01),LC3-Ⅱ蛋白表达水平明显升高(P<0.01),10μmol·L^(-1)褪黑素组自噬泡荧光强度升高(P<0.05)。与10μmol·L^(-1)褪黑素组比较,1μmol·L^(-1)褪黑素组和Luzindole组SH-SY5Y细胞存活率明显降低(P<0.05);1和5μmol·L^(-1)褪黑素组及Luzindole组SH-SY5Y细胞中ROS水平和细胞凋亡率升高(P<0.05),SH-SY5Y细胞中LC3-Ⅱ表达水平明显降低(P<0.05或P<0.01);1μmol·L^(-1)褪黑素组和Luzindole组自噬泡荧光强度降低(P<0.05)。结论:褪黑素可以抑制H_(2)O_(2)诱导的SH-SY5Y细胞氧化应激损伤,具有神经保护作用,其机制可能与降低ROS水平和提高细胞自噬有关。

高职院校课程建设质量标准开发和设计研究——以贵州省高职院校为例

以"高职院校课程建设质量标准内涵"为逻辑起点,从贵州区域社会发展、产业结构优化升级、行业企业人才需求及高职院校实际出发,参考"欧盟远程教育大学联盟"(EADTU)等发达国家及地区通行、科学而规范的教育质量标准,以"6个一级质量目标"为开发基础,以"28个二级质量目标"为拓展维度,共设计与开发了贵州省高职院校课程建设进程中的87条质量标准。

Review of transition paths for coal-fired power plants

The energy sector has an essential role in limiting the global average temperature increase to below 2°C.Redirecting and advancing technological progress contribute to carbon-free transition solutions.Energy transition is currently one of the most debated issues in the world.This paper reviews and summarizes the current policy projections and their assumptions organized by some major countries in the energy sector,particularly in the coal sector,and provides a detailed discussion on specific and significant socio-technical pathways taken by countries to achieve zero-carbon targets.Their implementation involves restructuring the existing energy system and requires appropriate policy support and sufficient investment in infrastructure development and technological innovation.Some basic principles and countermeasures that have already been implemented by some major emitters,such as India and China,are also discussed,with different transformation pathways.Critical suggestions are also provided,such as implementing best practice policies at the national level,moving to more efficient transition strategies,national and regional cooperation,cross-border energy grid integration,and private sector involvement to reduce carbon emissions from coal-fired power plants,not only by reducing coal consumption but also by introducing various low carbon technologies.

Case-control study on regular Ba Duan Jin practice for patients with chronic neck pain

Purpose:To observe the long-term effects of regular Ba Duan Jin exercises for patients with chronic neck pain.Methods:Participants with chronic neck pain were instructed to perform
30 min/d of Ba Duan Jin exercises(n?36)or assigned to a control group(n?36)for six months.Data from a visual analogue scale,Northwick Park Neck Pain Questionnaire,and the 36-item Shortform Health Survey(SF-36)were collected before,and three and six months after intervention began.Results:Participants undergoing Ba Duan Jin exercises achieved significantly greater improvement than the controls in visual analogue scale,Northwick Park Neck Pain Questionnaire and SF-36 health transition scores(all p<0.05).However,no significant differences were observed in SF-36 physical or mental component summaries.Conclusion:Regular Ba Duan Jin practice reduces suffering and pain,and increases health satisfaction in individuals with chronic neck pain.

费托蜡改性制备特种蜡发展探析

以费托合成蜡为原料改性制备特种蜡系列产品,走精细化工之路,是煤制油企业由生产初级产品向高附加值精细化学品跨越的必经之路。根据费托蜡的特点,介绍了费托蜡在橡胶防护、热熔胶、熔模铸造、上光剂、电气、食品、化妆品及农业等领域的应用。

浅析智能化技术在电气工程自动化控制中的应用

现如今人们开始普遍的追求智能化和精致化生活,在此背景之下智能化技术逐渐开始向各个行业渗透,并对这些行业的智能化发展指明了方向。该种技术在电气工程行业的有效运用,因此时下加强对智能化技术的概念和应用的分析研究具有现实意义。本文在对智能化技术相关概念详细分析的基础之上,就其在电气工程自动化控制领域的应用进行深入剖析,希望本文提出的建议对智能化技术与电气工程的和谐发展有所帮助。

A production-cost-simulation-based method for optimal planning of the grid interconnection between countries with rich hydro energy

Cross-border grid interconnection is a critical means to achieve wide-area share of hydro and other clean energy.Economic benefit assessment of cross-border grid interconnection projects should be carefully performed during early stage.In this paper,a method based on cost-benefit economic assessment for optimal planning of cross-border grid interconnection is proposed.An economic index for comprehensively assessing the cost of a transmission project and its resulting benefits of more usage of hydro energy is designed first.A chronological production cost simulation model considering hydro energy spillage due to transmission congestion and thermal operational limitation is then proposed to calculate the economic index.A case study is performed using the proposed method to determine the optimal capacity of a potential transmission link between Brazil and Argentina,which have rich and complementary hydro energy resources.

基于能值生态足迹的新疆生态绩效研究——以绿洲区为例

基于新疆绿洲区2010—2019年的相关数据,运用能值生态足迹模型和生态绩效,综合测算该区域的可持续发展水平,以评估新疆生态系统的生态效率,提高区域管理的有效性,同时明确生态和社会方面相应措施的副作用。结果表明:新疆生态均衡指数随着时间的推移不断下降,2010—2014年一直处于1以上,2015年及以后处于1以下,至2019年下降到0.701;新疆的实际人均生态承载力在不断增加,人均生态足迹在逐渐减少;从总体上看,可更新资源的人均生态足迹在下降,2010—2012年农产品、畜产品、水产品等可更新资源的人均能值消耗均呈逐年上升趋势,2013年出现短暂的下降后继续上升,而到2015年开始逐年均匀下降,2017—2019年有一个大幅度的下降;2010—2019年,畜产品在总能值中占据约90%的比重,10 a来一直居于第一位,且远高于农产品和水产品,农产品能值所占比重基本保持在6%左右,水产品则在5%左右波动;随着人民生活水平的提高,年人均消耗能源产品能值逐年升高,2019年的年人均能源产品消耗能值比2010年翻了近一番。根据研究结果,提出了4点发展建议。

有限元分析法在焊带夹设计中的应用

该文以太阳能电池片串焊机中的核心部件焊带夹机构为对象,利用SOLIDWORKS三维建模软件建立了焊带夹机构的三维模型,对其进行了受力分析,并以ANSYS Workbench软件作为分析手段对其进行了静力学分析,验证了焊带夹机构的设计是合理可行的。

Percentage of beta 2 band power of quantitative pharmaco-electroencephalography decreases in propofol anesthetized rabbits A dose-dependent analysis

BACKGROUND： Quantitative pharmaco-electroencephalography can be used for studying the dose-effect and time-effect relationships of drugs affecting central nervous system. Therefore, it may become an effective means for monitoring the anesthetic degree of anesthetic drug in the operation. OBJECTIVE： To observe the dose-effect relationship of propofol influencing β2-band power of quantitative pharmaco-electroencephalography in rabbits. DESIGN： A randomized block design. SETTING： Department of Anesthesiology, Xuzhou Medical College. MATERIALS： Thirty-six healthy adult rabbits of either gender, weighing （2.4 ± 0.5 ） kg, of clean grade, were provided by the Laboratory Animal Center of Xuzhou Medical College. The involved rabbits were randomly divided into 3 groups with 12 in each by table of random digit： high-dose propofol group, moderate-dose propofol group, and low-dose propofol group. The protocol was carried out in accordance with animal ethics guidelines for the use and care of animals. METHODS： This study was carried out in the Department of Anesthesiology, Xuzhou Medical College between August 1999 and April 2000. Rabbits in the high-dose propofol group, moderate-dose propofol group, and low-dose propofol group were injected with 10, 5 and 2.5 mg/kg propofol （ZENECA Company, British, Batch No. 032000）, respectively. Before and after intravenous administration of propofol, percentage of β2-band power of quantitative pharmaco-electroencephalography was measured, and the latent and persistent periods when rabbit righting reflex disappeared were observed by quantitative pharmaco-electroencephalography and power spectrum analysis. MAIN OUTCOME MEASURES： （1） Percentage of β2-band power of quantitative pharmaco-electroencephalography. （2）Latent period and persistent period of abolition of righting reflex of rabbits. RESULTS： Thirty-six rabbits were involved in the final analysis. （1）Effect of propofol on righting reflex of rabbits： Righting reflex disappeared within 1 minute after the rabbits being intravenously injected with propofol. The higher dose, the shorter latent period （r = - 0.94, P 〈 0.01）, and the longer persistent period （r =0.79, P 〈 0.01）. Both latent period and persistent period had good correlation with propofol dose. （2） Effect of propofol on the percentage of β2-band power of quantitative pharmaco-electroencephalography： In the low-dose propofol group, no significant changes in the percentage of β2-band power of quantitative pharmaco-electroencephalography existed between before and after administration within 30 minutes （P 〉 0.05）. In the moderate-dose propofol group, the percentage of β2-band power of quantitative pharmaco-electroencephalography in each brain region except for left and right frontal areas was significantly decreased within 30 s to 5 minutes （P 〈 0.05）, and recovered to the level before administration 10 minutes later. In the high-dose propofol group, the percentage of β2-band power of quantitative pharmaco-electroencephalography in each brain region was significantly decreased from 20 s to 5 minutes after administration （P 〈 0.05 - 0.01）, and that was gradually recovered to the level before administration 5 to 10 minutes after administration. This tendency was basically the same as the changes of latent period and persistent period. CONCLUSION： Propofol decreases the percentage of β2-band power of quantitative pharmacoelectroencephalography in dose-dependent manner, It indicates that β2-band power might become one of indexes for reflecting the anesthetic degree of propofol,

Exchange-coupling-induced fourfold magnetic anisotropy in CoFeB/FeRh bilayer grown on SrTiO_(3)(001)

Exchange coupling across the interface between a ferromagnetic(FM)layer and an antiferromagnetic(AFM)or another FM layer may induce a unidirectional magnetic anisotropy and/or a uniaxial magnetic anisotropy,which has been extensively studied due to the important application in magnetic materials and devices.In this work,we observed a fourfold magnetic anisotropy in amorphous Co Fe B layer when exchange coupling to an adjacent Fe Rh layer which is epitaxially grown on an SrTiO_(3)(001)substrate.As the temperature rises from 300 K to 400 K,Fe Rh film undergoes a phase transition from AFM to FM phase,the induced fourfold magnetic anisotropy in the Co Fe B layer switches the orientation from the Fe Rh<110>to Fe Rh<100>directions and the strength is obviously reduced.In addition,the effective magnetic damping as well as the two-magnon scattering of the Co Fe B/Fe Rh bilayer also remarkably increase with the occurrence of magnetic phase transition of Fe Rh.No exchange bias is observed in the bilayer even when Fe Rh is in the nominal AFM state,which is probably because the residual FM Fe Rh moments located at the interface can well separate the exchange coupling between the below pinned Fe Rh moments and the Co Fe B moments.

A microvascular image analysis method for optical-resolution photoacoustic microscopy

Optical-resolution photoacoustic microscopy(OR-PAM)has been shown to be an excellent tool for high-resolution imaging of microvasculature,and quantitative analysis of the microvascula-ture can provide valuable information for the early diagnosis and treatment of various vascular-related diseases.In order to address the characteristics of weak signals,discontinuity and small diameters in photoacoustic microvascular images,we propose a method adaptive to the micro-vascular segmentation in photoacoustic images,including Hessian matrix enhancement and the morphological connection operators.The accuracy of our vascular segmentation method is quantitatively evaluated by the multiple criteria.To obtain more precise and continuous mi-crovascular skeletons,an improved skeleton extraction framework based on the multistencil fast marching(MSFM)method is developed.We carried out in vivo OR-PAM microvascular imaging in mouse ears and subcutaneous hepatoma tumor model to verify the correctness and superiority of our proposed method.Compared with the previous methods,our proposed method can extract the microvascular network more completely,continuously and accurately,and provide an ef-fective solution for the quantitative analysis of photoacoustic microvascular images with many small branches.

Effects of Different Wall-breaking Methods on Extraction of Solanesol from Stems and Leaves of Potato

In order to extract solanesol from potato stems and leaves more effectively and improve the extraction rate of solanesol, the same batch of potato stems and leaves harvested from Guyuan, Ningxia was selected as a research object, cell wall of potato stems and leaves was broken by enzymolysis with cellulase and high- speed shearing, and then reflux-extracted with 95% ethanol. Solanesol content was determined by HPLC, and extract yield was calculated. Extract yield and solanesol extraction rate was used as an index for comparison of difference between the 2 wall-breaking methods, so as to select the optimal wall-breaking method. The results showed that enzymolysis with cellulase exhibited extraction rate and extract yield of solanesol of 91.38% and 8.02%, respectively, which were better than those under high-speed shear emulsification technique. The enzymolysis wall-breaking method has the advantages of simple operation and strong feasibility.