High sensitive immunoassay platforms have gained intense attention due to their vital roles in early-stage disease diagnosis and therapeutic information feedback. Although random covalent-binding of antibody has been ...High sensitive immunoassay platforms have gained intense attention due to their vital roles in early-stage disease diagnosis and therapeutic information feedback. Although random covalent-binding of antibody has been widely adopted in immunoassays due to its simplicity and effectiveness, it readily loses its activity and fails to exhibit high antigen-binding capacity. In this work, copolymer of zwitterionic sulfobetaine methacrylate(SBMA) and glycidyl methacrylate(GMA) brushes were immobilized onto inert polypropylene(PP) via surface-initiated atom transfer radical polymerization(ATRP) based on biomimetic dopamine pretreatment. Subsequently, boronic acid(BA) groups were covalently bonded via active GMA units, followed by the introduction of oriented immobilization of antibody. Owing to the oriented immobilization of antibody facilitated by BA groups in polymer brush, the bioactivity of antibody is well preserved, which endows the surface with significantly enhanced antigen-binding capacity. Moreover, the existence of SBMA segments in polymer brushes renders the surface high resistance to nonspecific protein adsorption, significantly alleviating the signal interference of antigen recognition. This strategy could find potential applications in developing high sensitive immunoassay platforms based on the different substrates.展开更多
Cell behaviors are regulated by a dynamic and complex environment characterized by biophysical,mechanical and biochemical properties.However,most works regulate cell behaviors under static conditions or by external fa...Cell behaviors are regulated by a dynamic and complex environment characterized by biophysical,mechanical and biochemical properties.However,most works regulate cell behaviors under static conditions or by external factors.To control cell adhesion and proliferation with a dynamic and mechanical environment,we pattern the surface on self-healing copolymer P(MMA/nBA).The copolymer P(MMA/nBA)with the composition of 48/52(MMA/nBA)recovers nearly 100%of its original tensile strains after 86 h of recovery from deformation.The physical patterns on P(MMA/nBA)film are obtained over large areas and the size of the hole and the width of connecting bar are in line with the copper grid specifications.The patterned surface tends to be fiat after 12 h with almost 75%-80%recovery.Compared with cell incubation on polystyrene fiat and patterned surface of P(MMA/nBA)film without self-healing capability,the number and morphology of cells are well manipulated on the patterned surface of self-healing P(MMA/nBA)film.This approach provides a convenient method for dynamically regulating the cell behaviors on the surface of self-healing materials without chemical or biological modifications.展开更多
Despite the fact that numerous infection-resistant surfaces have been developed to prevent bacterial colonization and biofilm formation, developing a stable, highly antibacterial and easily produced surface remains a ...Despite the fact that numerous infection-resistant surfaces have been developed to prevent bacterial colonization and biofilm formation, developing a stable, highly antibacterial and easily produced surface remains a technical challenge. As a crucial structural component of biofilm, extracellular DNA(eDNA) can facilitate initial bacterial adhesion, subsequent development, and final maturation. Inspired by the mechanistic pathways of natural enzymes(deoxyribonuclease), here we report a novel antibacterial surface by employing cerium(Ce(Ⅳ)) ion to mimic theDNA-cleavage ability of natural enzymes. In this process, the coordination chemistry of plant polyphenols and metal ions was exploited to create an in situ metal-phenolic film on substrate surfaces. Tannic acid(TA) works as an essential scaffold and Ce(Ⅳ) ion acts as both a cross-linker and a destructor of eDNA. The Ce(Ⅳ)-TA modified surface exhibited highly enhanced bacteria repellency and biofilm inhibition when compared with those of pristine or Fe(Ⅲ)-TA modified samples. Moreover, the easily produced coatings showed high stability under physiological conditions and had nontoxicity to cells for prolonged periods of time. This as-prepared DNA-cleavage surface presents versatile and promising performances to combat biomaterial-associated infections.展开更多
Marine economy is seriously affected by marine biofouling, which has plagued people for thousands of years. Although various strategies have been developed to protect artificial surfaces against marine biofouling, cos...Marine economy is seriously affected by marine biofouling, which has plagued people for thousands of years. Although various strategies have been developed to protect artificial surfaces against marine biofouling, cost-effective biofouling-resistant coating is still a goal in pursue. Herein, a cost-effective liquid-infused porous slippery surface (LIPSS) was facilely prepared by using poly(styrene-b-(ethylene-co-butylene)-b-styrene) (SEBS) elastomer to form microsphere surfaces, followed by infusing fluorocarbon lubricants into the porous structure. The as-prepared slippery surfaces were characterized by static water contact angle, sliding velocity and sliding angle analysis. We also investigated the adhesion behavior of Escherichia coli (E. coli) and limnetic algae on different surfaces. It is confirmed that the slippery surfaces have better anti-biofouling properties than the porous SEBS reference. This cost-effective approach is feasible and easily produced, and may potentially be used as fouling-resistant surfaces.展开更多
基金financially supported by the Research Program Funds of Jilin University(Nos.419080500665 and 451170301076)
文摘High sensitive immunoassay platforms have gained intense attention due to their vital roles in early-stage disease diagnosis and therapeutic information feedback. Although random covalent-binding of antibody has been widely adopted in immunoassays due to its simplicity and effectiveness, it readily loses its activity and fails to exhibit high antigen-binding capacity. In this work, copolymer of zwitterionic sulfobetaine methacrylate(SBMA) and glycidyl methacrylate(GMA) brushes were immobilized onto inert polypropylene(PP) via surface-initiated atom transfer radical polymerization(ATRP) based on biomimetic dopamine pretreatment. Subsequently, boronic acid(BA) groups were covalently bonded via active GMA units, followed by the introduction of oriented immobilization of antibody. Owing to the oriented immobilization of antibody facilitated by BA groups in polymer brush, the bioactivity of antibody is well preserved, which endows the surface with significantly enhanced antigen-binding capacity. Moreover, the existence of SBMA segments in polymer brushes renders the surface high resistance to nonspecific protein adsorption, significantly alleviating the signal interference of antigen recognition. This strategy could find potential applications in developing high sensitive immunoassay platforms based on the different substrates.
基金This work was financially supported by the National Key Research and Development Program of China(No.2016YFC1100402)the National Natural Science Foundation of China(Nos.51973222 and 51573186)+1 种基金the Natural Science Foundation of Jilin Province of China(Nos.20180101178JC and 20190701030GH)Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry,CIAC,CAS(Nos.201826 and 201715).
文摘Cell behaviors are regulated by a dynamic and complex environment characterized by biophysical,mechanical and biochemical properties.However,most works regulate cell behaviors under static conditions or by external factors.To control cell adhesion and proliferation with a dynamic and mechanical environment,we pattern the surface on self-healing copolymer P(MMA/nBA).The copolymer P(MMA/nBA)with the composition of 48/52(MMA/nBA)recovers nearly 100%of its original tensile strains after 86 h of recovery from deformation.The physical patterns on P(MMA/nBA)film are obtained over large areas and the size of the hole and the width of connecting bar are in line with the copper grid specifications.The patterned surface tends to be fiat after 12 h with almost 75%-80%recovery.Compared with cell incubation on polystyrene fiat and patterned surface of P(MMA/nBA)film without self-healing capability,the number and morphology of cells are well manipulated on the patterned surface of self-healing P(MMA/nBA)film.This approach provides a convenient method for dynamically regulating the cell behaviors on the surface of self-healing materials without chemical or biological modifications.
基金financially supported by the Research Program Funds of Jilin University (Nos.419080500665 and 451170301076)the Natural Science Foundation of Shandong Province (No.ZR2015EM036)
文摘Despite the fact that numerous infection-resistant surfaces have been developed to prevent bacterial colonization and biofilm formation, developing a stable, highly antibacterial and easily produced surface remains a technical challenge. As a crucial structural component of biofilm, extracellular DNA(eDNA) can facilitate initial bacterial adhesion, subsequent development, and final maturation. Inspired by the mechanistic pathways of natural enzymes(deoxyribonuclease), here we report a novel antibacterial surface by employing cerium(Ce(Ⅳ)) ion to mimic theDNA-cleavage ability of natural enzymes. In this process, the coordination chemistry of plant polyphenols and metal ions was exploited to create an in situ metal-phenolic film on substrate surfaces. Tannic acid(TA) works as an essential scaffold and Ce(Ⅳ) ion acts as both a cross-linker and a destructor of eDNA. The Ce(Ⅳ)-TA modified surface exhibited highly enhanced bacteria repellency and biofilm inhibition when compared with those of pristine or Fe(Ⅲ)-TA modified samples. Moreover, the easily produced coatings showed high stability under physiological conditions and had nontoxicity to cells for prolonged periods of time. This as-prepared DNA-cleavage surface presents versatile and promising performances to combat biomaterial-associated infections.
基金supported by the NationalN atural Science Foundation of China(No.51473167)the Natural Science Foundation of Heilongjiang Province of China(No.E201419)+1 种基金the Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry,Changchun Institute of Applied Chemistry,Chinese Academy of SciencesChina Postdoctoral Science Foundation(No.2016M602106)
文摘Marine economy is seriously affected by marine biofouling, which has plagued people for thousands of years. Although various strategies have been developed to protect artificial surfaces against marine biofouling, cost-effective biofouling-resistant coating is still a goal in pursue. Herein, a cost-effective liquid-infused porous slippery surface (LIPSS) was facilely prepared by using poly(styrene-b-(ethylene-co-butylene)-b-styrene) (SEBS) elastomer to form microsphere surfaces, followed by infusing fluorocarbon lubricants into the porous structure. The as-prepared slippery surfaces were characterized by static water contact angle, sliding velocity and sliding angle analysis. We also investigated the adhesion behavior of Escherichia coli (E. coli) and limnetic algae on different surfaces. It is confirmed that the slippery surfaces have better anti-biofouling properties than the porous SEBS reference. This cost-effective approach is feasible and easily produced, and may potentially be used as fouling-resistant surfaces.