Olfaction is primarily mediated by highly specified olfactory receptors （ORs）. Here, we cloned and identified an olfactory receptor, named SlituOR1 （Genbank no. JN835269）, from Spodoptera litura and found evidence...Olfaction is primarily mediated by highly specified olfactory receptors （ORs）. Here, we cloned and identified an olfactory receptor, named SlituOR1 （Genbank no. JN835269）, from Spodoptera litura and found evidence that it is a candidate pheromone receptor. It exhibited male-biased expression in the antennae, where it was localized at the base of sensilla trichoidea, the antennal sensilla mainly responsive to pheromones in moths. Conserved orthologues of this receptor, found among known pheromone receptors within the Lepidoptera, and SlituOR1 were placed among a clade of candidate pheromone re- ceptors in a phylogeny tree of insect OR gene sequences. SlituOR1 showed differential expression in S. litura populations attracted to traps baited with different ratios of the two sex pheromone components （9Z,11E）-tetradecadienyl acetate （Z9E11-14：OAc） and （9Z,12E）-tetradecadienyl acetate （Z9E12-14：OAc）. Knocking down of SlituOR1 by RNA interference reduced the electroantennogram （EAG） response to Z9E11-14：OAc, and this result is consistent with the field trapping experiment. We infer that variation in transcrip- tion levels of olfactory receptors may modulate sex pheromone perception in male moths and could provide some of the flexibility required to maintain the functionality of com- munication with females when a population is adapting to a new niche and reproductive isolation becomes an advantage.展开更多
A highly conserved and broadly expressed receptor protein Orco （olfactory coreceptor） is crucial for insect olfaction, and an orthologue of Orco has been identified in several insect species. Here we report the iden...A highly conserved and broadly expressed receptor protein Orco （olfactory coreceptor） is crucial for insect olfaction, and an orthologue of Orco has been identified in several insect species. Here we report the identification and characterization of Orco from Spodoptera litura. The protein displays high primary amino acid sequence conservation with other previously identified Orco orthologues. Bioinformatic analysis revealed that it has common features with other members of the Orco subfamily： seven-transmembrane domains with intracellular N-terminus and extracellular C-terminus. The transcript was detected in abundance in the chemosensory organs of the antennae of both male and female adults by real-time polymerase chain reaction analysis, and was localized at the bases of all categories of olfactory sensilla through in situ hybridization.展开更多
基金This study was funded by the Special Fund for Agro- scientific Research in the Public Interest in China （Grant No. 201203036）, Natural Science Foundation of Zhejiang （Project No. D3080388） and National 973 Project （Project No. 2012CB11410005）.
文摘Olfaction is primarily mediated by highly specified olfactory receptors （ORs）. Here, we cloned and identified an olfactory receptor, named SlituOR1 （Genbank no. JN835269）, from Spodoptera litura and found evidence that it is a candidate pheromone receptor. It exhibited male-biased expression in the antennae, where it was localized at the base of sensilla trichoidea, the antennal sensilla mainly responsive to pheromones in moths. Conserved orthologues of this receptor, found among known pheromone receptors within the Lepidoptera, and SlituOR1 were placed among a clade of candidate pheromone re- ceptors in a phylogeny tree of insect OR gene sequences. SlituOR1 showed differential expression in S. litura populations attracted to traps baited with different ratios of the two sex pheromone components （9Z,11E）-tetradecadienyl acetate （Z9E11-14：OAc） and （9Z,12E）-tetradecadienyl acetate （Z9E12-14：OAc）. Knocking down of SlituOR1 by RNA interference reduced the electroantennogram （EAG） response to Z9E11-14：OAc, and this result is consistent with the field trapping experiment. We infer that variation in transcrip- tion levels of olfactory receptors may modulate sex pheromone perception in male moths and could provide some of the flexibility required to maintain the functionality of com- munication with females when a population is adapting to a new niche and reproductive isolation becomes an advantage.
文摘A highly conserved and broadly expressed receptor protein Orco （olfactory coreceptor） is crucial for insect olfaction, and an orthologue of Orco has been identified in several insect species. Here we report the identification and characterization of Orco from Spodoptera litura. The protein displays high primary amino acid sequence conservation with other previously identified Orco orthologues. Bioinformatic analysis revealed that it has common features with other members of the Orco subfamily： seven-transmembrane domains with intracellular N-terminus and extracellular C-terminus. The transcript was detected in abundance in the chemosensory organs of the antennae of both male and female adults by real-time polymerase chain reaction analysis, and was localized at the bases of all categories of olfactory sensilla through in situ hybridization.
