BACKGROUND In recent years,confocal laser endomicroscopy(CLE)has become a new endoscopic imaging technology at the microscopic level,which is extensively performed for real-time in vivo histological examination.CLE ca...BACKGROUND In recent years,confocal laser endomicroscopy(CLE)has become a new endoscopic imaging technology at the microscopic level,which is extensively performed for real-time in vivo histological examination.CLE can be performed to distinguish benign from malignant lesions.In this study,we diagnosed using CLE an asymptomatic patient with poorly differentiated gastric adenocarcinoma.CASE SUMMARY A 63-year-old woman was diagnosed with gastric mucosal lesions,which may be gastric cancer,in the small curvature of the stomach by gastroscopy.She consented to undergo CLE for morphological observation of the gastric mucosa.Through the combination of CLE diagnosis and postoperative pathology,the intraoperative CLE diagnosis was considered to be reliable.According to our experience,CLE can be performed as the first choice for the diagnosis of gastric cancer.CONCLUSION CLE has several advantages over pathological diagnosis.We believe that CLE has great potential in the diagnosis of benign and malignant gastric lesions.展开更多
BACKGROUND Colorectal cancer(CRC)is the third most common malignancy of the digestive tract and the fifth leading cause of cancer-related mortality in China.Sporamin,a Kunitz-type trypsin inhibitor isolated from sweet...BACKGROUND Colorectal cancer(CRC)is the third most common malignancy of the digestive tract and the fifth leading cause of cancer-related mortality in China.Sporamin,a Kunitz-type trypsin inhibitor isolated from sweet potato,is a potential anti-cancer agent with activities against a number of malignant tumor cells in vitro.The liver secretes a myriad of endocrine factors that may facilitate the growth and transformation of tumors in the development of CRC.AIM To investigate the effects of sporamin on liver morphology and biomarkers of xenografted CRC in the liver of athymic BALB/c mice.METHODS Twenty-seven male BALB/c nude mice were randomly divided into control,vehicle,and sporamin groups.Mice in the latter two groups were intraperitoneally xenografted with LoVo colorectal carcinoma cells and intragastrically infused with saline or sporamin(0.5 g/kg body weight/d),respectively,for 3 wk.Hematoxylin and eosin(HE)staining of the sections was performed to observe morphological changes in hepatic tissue and real-time fluorescent quantitative PCR(qPCR)and enzyme-linked immunosorbent assay(ELISA)were used to measure the expression ofβ-catenin and vascular endothelial growth factor(VEGF)in the liver.RESULTS Sporamin significantly reduced the number and weight of tumor nodules formed in the abdominal cavity.Compared with the vehicle group,the mean tumor weight(±SD)in the sporamin group was significantly reduced(0.44±0.10 g vs 0.26±0.15 g)and the total number of tumors decreased from 93 to 55.HE staining showed that enlargement of the nucleus and synthesis of proteins within hepatocytes,as well as infiltration of inflammatory cells into the liver,were attenuated by sporamin.Immunohistochemical staining and ELISA showed that the concentrations ofβ-catenin and VEGF in the liver were significantly reduced by sporamin.Compared with the vehicle group,the expression ofβ-catenin measured in integrated optical density units per area was reduced in the sporamin group(47.29±9.10 vs 26.14±1.72;P=0.003).Expression of VEGF was also reduced after sporamin intervention from 20.78±2.06 in the vehicle group to 15.80±1.09 in the sporamin group(P=0.021).Compared with the vehicle group,the concentration ofβ-catenin decreased from 134.42±22.04 pg/mL to 109.07±9.65 pg/mL after sporamin intervention(P=0.00002).qPCR indicated that compared to the vehicle group,relative mRNA expression ofβ-catenin and VEGF in the liver of mice in the sporamin-treated group was significantly reduced to 71%±1%(P=0.000001)and 23%±7%(P=0.00002),respectively,of the vehicle group levels.CONCLUSION Sporamin down-regulates the expression and secretion ofβ-catenin and VEGF in the liver,which subsequently inhibits the transcription of downstream genes involved in cancer progression and angiogenesis.展开更多
BACKGROUND In this case study,a minimally invasive transalveolar approach using platelet-rich fibrin and bone substitute with simultaneous implantation was carried out in an elderly patient.We analyzed the cone-beam c...BACKGROUND In this case study,a minimally invasive transalveolar approach using platelet-rich fibrin and bone substitute with simultaneous implantation was carried out in an elderly patient.We analyzed the cone-beam computed tomography(CBCT)findings to evaluate bone regeneration.CASE SUMMARY A 65-year-old female with no contraindications for dental implants and loss of maxillary bilateral molars is described.Examination by CBCT showed the available vertical bone height in the bilateral posterior maxilla was 0.5-6.8 mm in the left and 2.8-6.5 mm in the right.The patient underwent a transalveolar approach using platelet-rich fibrin and bone substitute with simulataneous placement of an implant 10 mm in length.Six months post-surgery,the implant showed excellent osseointegration with the bone graft.Thereafter,full-ceramic crowns were fitted.Follow-up at 2 years demonstrated satisfactory prognosis.CONCLUSION Platelet-rich fibrin and bone substitute can be used to augment the maxillary sinus with a vertical bone height less than 4 mm.展开更多
A phytochemical investigation on Isodon flavidus led to the isolation of flavidanolide A(1),a rearranged diterpenoid featuring a six/seven/five-membered tricyclic skeleton,together with flavidanolide B(2),an uncommon ...A phytochemical investigation on Isodon flavidus led to the isolation of flavidanolide A(1),a rearranged diterpenoid featuring a six/seven/five-membered tricyclic skeleton,together with flavidanolide B(2),an uncommon heterodimeric diterpenoid consisting of a norabietane and a seco-isopimarane monomeric units.Their structures were elucidated by extensive spectroscopic data and single-crystal X-ray diffraction analyses.Their plausible biosynthetic routes were also proposed.In the bioassay,flavidanolide B was found to exhibit good inhibitory effect against lipopolysaccharide(LPS)-induced nitric oxide(NO)production in RAW264.7 cells comparable to positive control pyrrolidinedithiocarbamate ammonium(PDTC),which provided evidence for the medicinal value of I.flavidus as a folk medicine for treating inflammatory diseases.展开更多
The Colorado potato beetle （CPB）, Leptinotarsa decemlineata （Say）, is an infamous invasive species worldwide that aggressively attacks potato and other Solanaceae crops. CPB was first found in China in 1993 and ha...The Colorado potato beetle （CPB）, Leptinotarsa decemlineata （Say）, is an infamous invasive species worldwide that aggressively attacks potato and other Solanaceae crops. CPB was first found in China in 1993 and has since spread across 2.77 × 105 km2 in Xinjiang Uygur Autonomous Region. To better understand genetic variation and migration patterns, we used seven polymorphic microsatellite loci to elucidate the genetic relationships and gene flow among 10 CPB populations across Xinjiang. （i） Overall low levels of genetic diversity were detected on the entire population in Xinjiang but most of the diversity was retained among populations during invasion. （ii） The mean pairwise FST was low （0.071 ± 0.043） among populations. The genetic differentiation was little （pairwise FST 0.038 ± 0.016） between the five interior populations （Wusu, Urumqi, Jimsar, Qitai and Mulei） and Tacheng population. The six populations might come from the same genetic group via Bayesian clustering and were closely related on a neighbor-joining tree. Combining the history data, the five interior populations may have originated from Tacheng. （iii） Gene flow was frequent, especially among the five interior populations. Individuals from the interior populations could be assigned to Tacheng at higher probabilities （means 0.518 ± 0.127） than vice versa （means 0.328 ± 0.074）, suggesting that the beetle population has spread from the border to the interior in Xinjiang.展开更多
基金The Health Science and Technology Foundation of Inner Mongolia,No.202201436Science and Technology Innovation Foundation of Inner Mongolia,No.CXYD2022BT01.
文摘BACKGROUND In recent years,confocal laser endomicroscopy(CLE)has become a new endoscopic imaging technology at the microscopic level,which is extensively performed for real-time in vivo histological examination.CLE can be performed to distinguish benign from malignant lesions.In this study,we diagnosed using CLE an asymptomatic patient with poorly differentiated gastric adenocarcinoma.CASE SUMMARY A 63-year-old woman was diagnosed with gastric mucosal lesions,which may be gastric cancer,in the small curvature of the stomach by gastroscopy.She consented to undergo CLE for morphological observation of the gastric mucosa.Through the combination of CLE diagnosis and postoperative pathology,the intraoperative CLE diagnosis was considered to be reliable.According to our experience,CLE can be performed as the first choice for the diagnosis of gastric cancer.CONCLUSION CLE has several advantages over pathological diagnosis.We believe that CLE has great potential in the diagnosis of benign and malignant gastric lesions.
基金Supported by the National Natural Science Foundation of China,No.81573128 and No.81703216
文摘BACKGROUND Colorectal cancer(CRC)is the third most common malignancy of the digestive tract and the fifth leading cause of cancer-related mortality in China.Sporamin,a Kunitz-type trypsin inhibitor isolated from sweet potato,is a potential anti-cancer agent with activities against a number of malignant tumor cells in vitro.The liver secretes a myriad of endocrine factors that may facilitate the growth and transformation of tumors in the development of CRC.AIM To investigate the effects of sporamin on liver morphology and biomarkers of xenografted CRC in the liver of athymic BALB/c mice.METHODS Twenty-seven male BALB/c nude mice were randomly divided into control,vehicle,and sporamin groups.Mice in the latter two groups were intraperitoneally xenografted with LoVo colorectal carcinoma cells and intragastrically infused with saline or sporamin(0.5 g/kg body weight/d),respectively,for 3 wk.Hematoxylin and eosin(HE)staining of the sections was performed to observe morphological changes in hepatic tissue and real-time fluorescent quantitative PCR(qPCR)and enzyme-linked immunosorbent assay(ELISA)were used to measure the expression ofβ-catenin and vascular endothelial growth factor(VEGF)in the liver.RESULTS Sporamin significantly reduced the number and weight of tumor nodules formed in the abdominal cavity.Compared with the vehicle group,the mean tumor weight(±SD)in the sporamin group was significantly reduced(0.44±0.10 g vs 0.26±0.15 g)and the total number of tumors decreased from 93 to 55.HE staining showed that enlargement of the nucleus and synthesis of proteins within hepatocytes,as well as infiltration of inflammatory cells into the liver,were attenuated by sporamin.Immunohistochemical staining and ELISA showed that the concentrations ofβ-catenin and VEGF in the liver were significantly reduced by sporamin.Compared with the vehicle group,the expression ofβ-catenin measured in integrated optical density units per area was reduced in the sporamin group(47.29±9.10 vs 26.14±1.72;P=0.003).Expression of VEGF was also reduced after sporamin intervention from 20.78±2.06 in the vehicle group to 15.80±1.09 in the sporamin group(P=0.021).Compared with the vehicle group,the concentration ofβ-catenin decreased from 134.42±22.04 pg/mL to 109.07±9.65 pg/mL after sporamin intervention(P=0.00002).qPCR indicated that compared to the vehicle group,relative mRNA expression ofβ-catenin and VEGF in the liver of mice in the sporamin-treated group was significantly reduced to 71%±1%(P=0.000001)and 23%±7%(P=0.00002),respectively,of the vehicle group levels.CONCLUSION Sporamin down-regulates the expression and secretion ofβ-catenin and VEGF in the liver,which subsequently inhibits the transcription of downstream genes involved in cancer progression and angiogenesis.
基金Supported by the Jilin Province Science and Technology Development Plan Project,No.20180101123JC13th Five-Year Science and Technology Project of Jilin Provincial Education Department,No.JJKH20190096KJ+2 种基金Jilin Province Health and Health Technology Innovation Project,No.2018J072Project of Jilin Provincial Development and Reform Commission,No.2019C051-2and Jilin Province TCM Science and Technology Project,No.2019036。
文摘BACKGROUND In this case study,a minimally invasive transalveolar approach using platelet-rich fibrin and bone substitute with simultaneous implantation was carried out in an elderly patient.We analyzed the cone-beam computed tomography(CBCT)findings to evaluate bone regeneration.CASE SUMMARY A 65-year-old female with no contraindications for dental implants and loss of maxillary bilateral molars is described.Examination by CBCT showed the available vertical bone height in the bilateral posterior maxilla was 0.5-6.8 mm in the left and 2.8-6.5 mm in the right.The patient underwent a transalveolar approach using platelet-rich fibrin and bone substitute with simulataneous placement of an implant 10 mm in length.Six months post-surgery,the implant showed excellent osseointegration with the bone graft.Thereafter,full-ceramic crowns were fitted.Follow-up at 2 years demonstrated satisfactory prognosis.CONCLUSION Platelet-rich fibrin and bone substitute can be used to augment the maxillary sinus with a vertical bone height less than 4 mm.
基金supported by the National Natural Science Foundation of China(No.82204605)the National Natural Science Foundation of China(No.81560709)+3 种基金the Technology Fund of Guizhou Administration of Traditional Chinese Medicine(No.QZYY-2022019)Science and Technology Tip-top Talent Foundation of Universities in Guizhou Province(No.Qian jiao he KY(2021)034)the Research Grant Council of the Hong Kong Special Administrative Region,China(No.HKBU 12102219)the University Grants Committee of the Hong Kong Special Administrative Region,China(UGC Research Matching Grant Scheme,No.RMG2019_1_19)。
文摘A phytochemical investigation on Isodon flavidus led to the isolation of flavidanolide A(1),a rearranged diterpenoid featuring a six/seven/five-membered tricyclic skeleton,together with flavidanolide B(2),an uncommon heterodimeric diterpenoid consisting of a norabietane and a seco-isopimarane monomeric units.Their structures were elucidated by extensive spectroscopic data and single-crystal X-ray diffraction analyses.Their plausible biosynthetic routes were also proposed.In the bioassay,flavidanolide B was found to exhibit good inhibitory effect against lipopolysaccharide(LPS)-induced nitric oxide(NO)production in RAW264.7 cells comparable to positive control pyrrolidinedithiocarbamate ammonium(PDTC),which provided evidence for the medicinal value of I.flavidus as a folk medicine for treating inflammatory diseases.
基金We thank Mr. De-Cheng Ma, Mr. Ainiwaer and Ms. Li Jing at the plant protection station of Xinjiang Uighur Autonomous Region for their help during field collections. We are grateful to Mr. Xiao-Long Chen, Mr. Shou- Qi Zhao, Mr. Ya-Kun Song and Ms. Qin Meng from the National Agro-Technical Extension and Service Centre, Ministry of Agriculture, PR China, for providing experi- mental instruments and research conditions. We express special thanks to Drs. Li Ren, Jing Xu and Zhi-Liang Wang of the Institute of Zoology, Chinese Academy of Sciences, for their help with field sampling and the experimental process. We also thank Qiao-Zhe Lou, Hong-Yu Zhu and Lei Du Lei for help with DNA manipulations and data analysis. This research was supported by the Chinese Academy of Sciences （Hundred Talents in 2010）, Ministry of Science and Technology （2009CB119204）, the Ministry of Agriculture （200903042）, and the National Natural Science Foundation of China （31172130, J0930004） programs.
文摘The Colorado potato beetle （CPB）, Leptinotarsa decemlineata （Say）, is an infamous invasive species worldwide that aggressively attacks potato and other Solanaceae crops. CPB was first found in China in 1993 and has since spread across 2.77 × 105 km2 in Xinjiang Uygur Autonomous Region. To better understand genetic variation and migration patterns, we used seven polymorphic microsatellite loci to elucidate the genetic relationships and gene flow among 10 CPB populations across Xinjiang. （i） Overall low levels of genetic diversity were detected on the entire population in Xinjiang but most of the diversity was retained among populations during invasion. （ii） The mean pairwise FST was low （0.071 ± 0.043） among populations. The genetic differentiation was little （pairwise FST 0.038 ± 0.016） between the five interior populations （Wusu, Urumqi, Jimsar, Qitai and Mulei） and Tacheng population. The six populations might come from the same genetic group via Bayesian clustering and were closely related on a neighbor-joining tree. Combining the history data, the five interior populations may have originated from Tacheng. （iii） Gene flow was frequent, especially among the five interior populations. Individuals from the interior populations could be assigned to Tacheng at higher probabilities （means 0.518 ± 0.127） than vice versa （means 0.328 ± 0.074）, suggesting that the beetle population has spread from the border to the interior in Xinjiang.
