Wedelolactone attenuates sepsis-associated acute liver injury by regulating the macrophage M1/M2 polarization balance through the PI3K/AKT/NF-κB signalling pathway

Background:Liver injury caused by sepsis seriously impairs the normal physiology of the liver.Wedelactone(WED)has an obvious anti-inflammatory effect against liver damage caused by various factors.Nevertheless,further research is needed to determine if WED might mitigate acute liver damage linked to sepsis by influencing macrophage polarization.Methods:We first assessed the effect of WED on lipopolysaccharides-triggered liver injury by biochemistry assay and tissue staining.Inflammatory factors were assessed using the ELISA kits.The expression of Cluster of Differentiation 86(CD86)and Cluster of Differentiation 206(CD206)was measured by immunofluorescence assay.The protein levels of inducible nitric oxide sythase(iNOS),Arginase 1(Arg-1),phosphatidylinositol 3-kinase(PI3K),protein kinase B(AKT),PI3K phosphorylation(p-PI3K),AKT phosphorylation(p-AKT),inhibitor of kappa B kinase(IKK),inhibitor of kappa B(IκB),and nuclear factor kappa-B(NF-κB)p65 were quantified by western blot analysis.Results:WED decreased the level of alanine aminotransferase(ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP)and malondialdehyde,and increased the activity of superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX).Moreover,WED exerted effective anti-inflammatory effects by decreasing the level of Tumor necrosis factor-α(TNF-α)and Interleukin 6(IL-6)and increasing the level of Interleukin 10(IL-10)in serum and cells.WED not only decreased CD86 and iNOS expression but also increased CD206 and Arg-1 expression.WED also downregulated the increased expression of PI3K,AKT,p-PI3K,p-AKT,IKK,and NF-κB p65 induced by lipopolysaccharides,while up-regulated the decreased expression of IκB.Besides,LY294002 with WED decreased the expression of protein PI3K,AKT,p-PI3K,p-AKT,IKK and NF-κB p65,and raised the expression of IκBα.Conclusion:Wedelolactone could attenuate sepsis-associated acute liver injury,and its mechanism may be associated with balancing pro-inflammatory and anti-inflammatory by the regulation of M1/M2 macrophage polarization via the PI3K/AKT/NF-κB signaling pathway.

联合运输中驱动液压系统群可靠性分析与计算

为保证联合运输安全,对两车联合运输的驱动液压系统组进行了研究。同步控制是安全运输的关键,对驱动液压系统群和控制系统的可靠性要求很高。因此,必须对驱动液压系统群的寿命预测和可靠性增长进行研究。两车联合运输的驱动液压系统群的可靠性为对象进行了研究,首先对某运输车辆的可靠性模型进行了研究,建立了运输车传动系统的可靠性框图模型;并在此基础上建立了相应的可靠性数学模型;经过MATLAB软件进行计算,得到了驱动液压系统群的可靠性曲线和可靠度。并针对工程实践中的驱动不同步问题建立了驱动液压系统群的故障树模型,对故障树进行了定量和定性分析,最终找出了驱动液压系统的薄弱环节。进而可以在设计过程中就考虑到预防故障的产生,提高系统的设计水平,该方法可有效提高联合运输液压系统群可靠性。

再制造盾构机刀盘液压驱动系统的可靠性分析

再制造作为绿色制造的重要组成环节,是解决资源短缺和环境污染问题的有效途径之一。盾构机刀盘与其它部件相比,有着对可靠性要求特别高的特点,因此开展盾构再制造刀盘的可靠性研究十分必要。考虑到盾构机的掘进工况,建立刀盘液压驱动系统的可靠性框图与数学模型,通过仿真得到的可靠度曲线进行了可靠性分析。建立马达模块的故障树模型并对马达故障树进行分析,找到了导致马达失效的关键因素,为提高盾构液压驱动系统的可靠性提供了理论措施。

Surgical outcome and patient satisfaction after Z-epicanthoplasty and blepharoplasty

AIM: To evaluate surgical outcomes of modified Z-epicanthoplasty with blepharoplasty that we previously reported from the patient’s perspective using patient-reported outcome measures(PROMs) and patient satisfaction scores.METHODS: A total of patients(n=180) who underwent the surgery between January 2013 and June 2016 were randomly selected. Standardized patient satisfaction forms(total score, 40) and validated PROMs questionnaires(total score, 12) were sent to patients for completion. PROMs assesses the severity of scarring, pain and asymmetry, as well as functional and appearance issues.RESULTS: All patients were female, ranging from 18 to 35 years old(mean=24). The response rate was 73.3%(n=132). The majority of patients reported good or excellent outcomes based on PROM analysis. Patients reported minimum or non-visible scarring at both the double eyelid surgical scar(85.6%) and the inner canthus(80.3%). Issues concerning function and appearance were minimal as 80.3% reported satisfaction with both domains. Notably, the majority of patients reported either a high or very high satisfaction rate to yield a mean score of 104 out of 120(P<0.05).CONCLUSION: Integration of our modified Z-epicanthoplasty with blepharoplasty produces good outcomes based on PROM results, which shows a positive linear relationship with patient satisfaction scores.

Responses from two firing patterns in inferior colliculus neurons to stimulation of the lateral lemniscus dorsal nucleus

The γ-aminobutyric acid neurons（GABAergic neurons） in the inferior colliculus are classified into various patterns based on their intrinsic electrical properties to a constant current injection. Although this classification is associated with physiological function, the exact role for neurons with various firing patterns in acoustic processing remains poorly understood. In the present study, we analyzed characteristics of inferior colliculus neurons in vitro, and recorded responses to stimulation of the dorsal nucleus of the lateral lemniscus using the wholecell patch clamp technique. Seven inferior colliculus neurons were tested and were classified into two firing patterns： sustained-regular（n = 4） and sustained-adapting firing patterns（n = 3）. The majority of inferior colliculus neurons exhibited slight changes in response to stimulation and bicuculline. The responses of one neuron with a sustained-adapting firing pattern were suppressed after stimulation, but recovered to normal levels following application of the γ-aminobutyric acid receptor antagonist. One neuron with a sustained-regular pattern showed suppressed stimulation responses, which were not affected by bicuculline. Results suggest that GABAergic neurons in the inferior colliculus exhibit sustained-regular or sustained-adapting firing patterns. Additionally, GABAergic projections from the dorsal nucleus of the lateral lemniscus to the inferior colliculus are associated with sound localization. The different neuronal responses of various firing patterns suggest a role in sound localization. A better understanding of these mechanisms and functions will provide better clinical treatment paradigms for hearing deficiencies.

Degradation of warm permafrost and talik formation on the Qinghai-Tibet Plateau in 2006-2021

Permafrost is degrading globally,particularly those with low thermal stability on the Qinghai-Tibet Plateau,owing to climate change.However,the inadequacy of direct research on permafrost degradation based on in-situ monitoring limits the prediction of permafrost degradation and engineering practices.This study explored the processes and modes of permafrost degradation into talik by analyzing ground temperature data from five points in the hinterland of the Qinghai-Tibet Plateau from 2006 to 2021.The results showed that the degradation of the warm permafrost layer with a geothermal gradient of zero occurred simultaneously in the top and bottom directions.The rate of permafrost degradation from the top down and bottom up increase during the degradation process,but the increase of the former is more drastic after the formation of thawed interlayer.Additionally,the construction of the Qinghai-Tibet Railway changed the degradation modes of the permafrost in adjacent natural sites through horizontal heat transfer,particularly after through talik formation under the embankment.The findings suggest that taking countermeasures before or immediately after forming thawed interlayer is more effective.When evaluating the thermal impact of projects in warm permafrost regions,special attention should be given to the horizontal heat transfer process that may result from the formation of a through talik.

Comparative pharmacokinetic studies of borneol in mouse plasma and brain by different administrations

Borneol,a monoterpenoid alcohol,is used widely,particularly in combined formulas for preventing and curing cardiovascular and cerebrovascular diseases in traditional Chinese medicine.In order to understand the blood and brain pharmacokinetics after intravenous,intranasal,or oral administration and to investigate the superiority and feasibility of intranasal administration,a simple gas chromatographic (GC) method with flame ionization detection (FID) was developed for the quantification of borneol.Blood samples and brain were collected from mice at 1,3,5,10,20,30,60,90,and 120 min after intravenous,intranasal,or oral administration of borneol at a dosage of 30.0 mg/kg.Sample preparations were carried out by liquid-liquid extraction with an internal standard solution of octadecane.The pharmacokinetic parameters were calculated by the software of Kinetica.The calibration curves were linear in the range of 0.11-84.24 μg/ml and 0.16-63.18 μg/g for borneol in plasma and brain,respectively.The methodological and extraction recoveries were both in the range of 85%-115%.The intra-day and inter-day variabilities for plasma and brain samples were ≤5.00% relative standard deviation (RSD).The absolute bioavailabilities F of intranasal and oral administrations were 90.68% and 42.99%.The relative brain targeted coefficients Re of intranasal and oral administrations were 68.37% and 38.40%.The GC-FID method developed could be applied to determination and pharmacokinetic study.The borneol from injection was distributed and metabolized fast without absorption process.The borneol from oral administration was distributed more slowly and had the lowest absolute bioavailability.Nasal administration of borneol was quickly absorbed into the blood and brain,was easy to use and had a greater safety than infection,which makes it worthy of further development as an administration route for encephalopathy treatment.

Digitization modeling of a CGEM detector based on Garfield++simulation

Background The Cylindrical Gas ElectronMultiplier Inner Tracker(CGEM-IT)is one of the candidates for the replacement of the current BESIII inner drift chamber,which has developed serious aging effects due to beam-related backgrounds.Purpose To build a full digitization model,it is necessary to understand the electron drift behavior and signal induction.Methods A simulation study based on Garfield++and ANSYS software has been performed.Results Parameters describing the Lorentz angle,diffusion effect,drift time,multiplication,and induction are obtained from the Garfield++simulation.Based on these results,a preliminary digitization model is implemented in the BESIII offline software system.