Comparison of different methods of intestinal obstruction in a rat model

AIM:To investigate different methods of creating incomplete intestinal obstruction in a rat model and to compare their electrophysiologic,morphologic and histologic characteristics. METHODS:Rat ileum was partially obstructed by the respective application of:braided silk(penetrated the mesentery and surrounded intestine);half ligation (penetrated directly and ligated 1/2 cross-section of the intestine);wide pipe(6 mm in width,surrounded the intestine);narrow pipe(2 mm in width,surrounded the intestine).A control was also included(no obstruction). Various behavioral and electrophysiologic variables, as well as morphologic and immunohistochemical observations were recorded by blinded investigators at different time points(12,24,48,72 h),including daily general condition,ileal wet weight and circumference, macromorphous and micromorphous intestine,bowel movement capability in vivo and in vitro,slow wave and neural electrical activity,and the number of c-Kit positive interstitial cells of Cajal(ICC). RESULTS:Despite being of a similar general condition, these methods resulted in different levels of obstruction in each group compared with the control at different time points(12,24,48,72 h).However,these fields of the wide pipe rat showed significantly differences when compared with the other three obstructed groups at 12 to 72 h,including macroscopic and histological presentation,intestinal transit ratio and contractility,circumference and wet weight,amplitude and frequency of nerve electrical discharge and slow wave,and ICC numbers(all P<0.01). CONCLUSION:The wide pipe rat method is significantly more reliable and stable than the other methods of obstruction,demonstrating that use of the wide pipe method can be a useful model of incomplete intestinal obstruction.

Ethical inspection about laboratory animals

Laboratory animals and animal experiments are foundations and important support conditions for life sciences, especially for medical research. The animal experiments have drawn extensive attention from the society because of the ethical issue. This paper takes Wenzhou Medical University as an example to give a brief introduction to the ethical review about laboratory animals in the university so as to further draw attention and concerns from the public about the ethical issue of laboratory animals. We successively introduce its scientific projects, nurturing environment and ethical review of laboratory animals.

Complement C3 Aggravates Post-epileptic Neuronal Injury Via Activation of TRPV1

Epilepsy is a brain condition characterized by the recurrence of unprovoked seizures.Recent studies have shown that complement component 3(C3)aggravate the neuronal injury in epilepsy.And our previous studies revealed that TRPV1(transient receptor potential vanilloid type 1)is involved in epilepsy.Whether complement C3 regulation of neuronal injury is related to the activation of TRPV1 during epilepsy is not fully understood.We found that in a mouse model of status epilepticus(SE),complement C3 derived from astrocytes was increased and aggravated neuronal injury,and that TRPV 1-knockout rescued neurons from the injury induced by complement C3.Circular RNAs are abundant in the brain,and the reduction of circRad52 caused by complement C3 promoted the expression of TRPV 1 and exacerbated neuronal injury.Mechanistically,disorders of neuron-glia interaction mediated by the C3-TRPV1 signaling pathway may be important for the induction of neuronal injury.This study provides support for the hypothesis that the C3-TRFV1 pathway is involved in the prevention and treatment of neuronal injury and cognitive disorders.

Clinical characteristics and plasma antibody titer of patients with COVID-19 in Zhejiang,China

目的:利用胶体金快速检测试剂盒测定恢复期患者血浆中IgG/IgM的抗体滴度,回顾性分析新型冠状病毒肺炎(COVID-19)患者临床特征、抗体效价变化特点,以及两者间的相关性.创新点:利用胶体金检测试剂盒检测恢复期血浆抗体滴度,结合重症及危重症患者病例特点及实验室检测指标分析血浆抗体效价变化规律,及其临床特征的关系,为临床重症及危重症患者诊治以及筛选恢复期血浆供者提供参考依据.方法:收集293份来自COVID-19康复者随访过程中的血液标本,取血浆原液,用生理盐水按不同比例(1:80、1:160、1:320和1:640)稀释后进行胶体金试纸法IgG/IgM抗体检测,并记录相应患者检验指标水平.通过电子病历系统检索回顾性分析温州市第六人民医院截至2020年2月24日确诊的COVID-19患者的流行病学特征和临床信息.通过线性回归分析两变量之间的相关性,P<0.05时具有统计学意义.结论:症状出现4~6周时,COVID-19患者恢复期血浆IgG滴度水平可达到1:320~1:640或者更高水平.此时部分患者的血浆IgM仍为阳性.COVID-19患者恢复期血浆IgM抗体滴度检测结果显示男性较女性高.本研究中危重症患者均需要无创通气来维持生命,且重症和极危重症患者均易发生水、电解质紊乱.COVID-19发病早期发生凝血功能障碍及炎症,与恢复期血浆中IgG和IgM的抗体滴度水平存在一定相关性.