CASC15 Gene Polymorphisms and Glioma Susceptibility in Chinese Children

Objective:Gliomas are the most common tumors in the central nervous system.The cancer susceptibility candidate 15(CASC15)gene has been reported to be a susceptibility gene for several types of cancer.No studies have been carried out on the predisposing effect of CASC15 gene single nucleotide polymorphisms(SNPs)on glioma risk.Methods:In order to determine whether CASC15 gene SNPs are involved in glioma susceptibility,the first association study in a relatively large sample,which consisted of 171 patients and 228 healthy controls recruited from China,was performed.The contribution of SNPs(rs6939340 A>G,rs4712653 T>C and rs9295536 C>A)to the risk of glioma was evaluated by multinomial logistic regression,based on the calculation of the odds ratio(OR)and 95%confidence interval(CI).Results:In the single locus and combined analysis,it was revealed that the genetic risk score had no significant associations between CASC15 gene SNPs and glioma risk.However,in the stratified analysis,a significant decrease in risk of glioma was observed in subjects of<60 months old with the rs4712653 TT genotype,when compared to those with the CC/CT genotype(OR=0.12,95%CI=0.02-0.91,P=0.041).Conclusion:The present study provides referential evidence on the association between the genetic predisposition of the CASC15 gene and glioma risk in Chinese children.However,more well-designed case-control studies and functional experiments are needed to further explore the role of CASC15 gene SNPs.

Application value of electrocardiogram in the treatment and prognosis of idiopathic pulmonary hypertension

Objective: To evaluate the treatment and prognostic value of 12-lead electrocardiogram in idiopathic pulmonary hypertension (IPAH). Methods: A total of 200 patients with initial diagnosis of IPAH were included to analyze the correlation between ECG variables and hemodynamics.Univariate and multivariate proportional hazards regression analysis (Cox regression analysis) were used to analyze the changes of ECG parameters and determine the ECG parameters related to IPAH patients. Results: Partial correlation analysis showed that the amplitude of R wave in V1 lead was correlated with mPAP(r=0.343, P<0.001), and the sum of the amplitude of R wave in V1 lead and S wave in V5 lead was correlated with mPAP(r=0.504, P<0.001). The amplitude of R wave in V1 lead was correlated with mortality (HR 3.154, P<0.001), and the sum of the amplitude of R wave in V1 lead and S wave in V5 lead was correlated with mortality (HR 5.056, P<0.001). After 3 months of treatment,ECG parameters and hemodynamic status were improved. Conclusion: The specific 12-lead ECG parameters can reflect the improvement of the treatment of patients with idiopathic pulmonary hypertension and help to evaluate the prognosis of patients with idiopathic pulmonary hypertension.

Effects of Acupuncture at Tianshu(ST25)and Shangjuxu(ST37)on the Intestinal Mucosa and Metabolites of Local Skin Tissues at Tianshu(ST25)in 2,4,6-Trinitrobenzene Sulfonic Acid and Ethanol-induced Rats

Objective:To observe the effect of acupuncture on local skin tissue metabolites of Tianshu(ST25)in rats with Crohn's disease(CD)using metabolomics and to explore the mechanism of acupuncture treatment metabolites and pathway on local skin tissue of Tianshu(ST25).Materials and Methods:Thirty-eight Sprague-Dawley rats were randomly assigned to a normal group(n=14)and a CD modeling group(n=24).Rats in the CD modeling group were administered with 2,4,6-trinitrobenzene sulfonic acid and ethanol enema,in addition to the normal group.Four normal rats and four CD modeling rats were selected at random after model identification was established.Furthermore,CD modeling group rats were randomly assigned to two groups of 10 rats:the model group and acupuncture group.Rats in the acupuncture group underwent acupuncture of Tianshu(ST25)and Shangjuxu(ST37)for 7 days.After the intervention,the colon tissue was collected from each group of rats and the pathological changes were observed through hematoxylin and eosin(HE)staining.The local skin tissues at Tianshu(ST25)of the rats in each group were taken,and the proton nuclear magnetic resonance technique was used to detect the metabonomics of the local skin tissues of Tianshu(ST25)in rats.Results:Compared with the normal group,the following were observed in the model group after HE staining:irregular colon morphology and other pathological changes such as intestinal mucosal hyperemia,edema,ulcers,polyps,and inflammatory cell infiltration.In the acupuncture group,colon tissue structure was relatively complete and layered,the colon gland structure was restored,and inflammatory cell infiltration was significantly improved.The choline,glycerin,glycine,guanidoacetic acid,and proline levels were significantly higher in the model group than in the normal group(P<0.05 or P<0.01).Compared with the model group,the acupuncture group had significantly increased contents of alanine,leucine,L-phenylalanine,and tyrosine in the skin(P<0.05 or P<0.01).Valine,leucine,and L-isoleucine biosynthesis and L-phenylalanine metabolism were the main metabolic pathways involved in the changes in the local skin tissues of the rats in each group,biosynthesis of tyrosine,and tryptophan.Conclusion:The change in the metabolites in the local area of Tianshu(ST25)may be related to intestinal disease.The acupuncture of Tianshu(ST25)can improve intestinal inflammatory reaction in rats with CD,and this finding may be related to the regulation of amino acid metabolites and their pathways in the local acupoint tissues of Tianshu(ST25)by acupuncture.

Association between genetic polymorphisms of base excision repair pathway and glioma susceptibility in Chinese children

Glioma is an aggressive malignant primary tumor in the central nervous system[1,2].Although the pathogenesis of glioma is unclear,the only identified environmental risk fac-tor is ionizing radiation[3].However,other factors also may alter the glioma risk,among which gene polymorphisms may play prominent role in glioma susceptibility.Base excision repair(BER)is a DNA repair mechanism that maintains genome integrity and guarantees DNA base modification properly.Many signifcant and common proteins involved in the BER pathway are associated with cancer.