The gut-brain axis is a bidirectional information interaction system between the central nervous system(CNS) and the gastrointestinal tract, in which gut microbiota plays a key role. The gut microbiota forms a complex...The gut-brain axis is a bidirectional information interaction system between the central nervous system(CNS) and the gastrointestinal tract, in which gut microbiota plays a key role. The gut microbiota forms a complex network with the enteric nervous system, the autonomic nervous system, and the neuroendocrine and neuroimmunity of the CNS, which is called the microbiota-gut-brain axis. Due to the close anatomical and functional interaction of the gut-liver axis, the microbiota-gut-liver-brain axis has attracted increased attention in recent years. The microbiota-gut-liver-brain axis mediates the occurrence and development of many diseases, and it offers a direction for the research of disease treatment. In this review, we mainly discuss the role of the gut microbiota in the irritable bowel syndrome, inflammatory bowel disease, functional dyspepsia, non-alcoholic fatty liver disease, alcoholic liver disease, cirrhosis and hepatic encephalopathy via the gut-liver-brain axis, and the focus is to clarify the potential mechanisms and treatment of digestive diseases based on the further understanding of the microbiota-gut-liver-brain axis.展开更多
Organic anion transporters(OATs)and organic anion transporter polypeptides(OATPs)are classified within two SLC superfamilies,namely,the SLC22A superfamily and the SLCO superfamily(formerly the SLC21A family),respectiv...Organic anion transporters(OATs)and organic anion transporter polypeptides(OATPs)are classified within two SLC superfamilies,namely,the SLC22A superfamily and the SLCO superfamily(formerly the SLC21A family),respectively.They are expressed in many tissues,such as the liver and kidney,and mediate the absorption and excretion of many endogenous and exogenous substances,including various drugs.Most are composed of 12 transmembrane polypeptide chains with the C-terminus and the N-terminus located in the cell cytoplasm.OATs and OATPs are abundantly expressed in the liver,where they mainly promote the uptake of various endogenous substrates such as bile acids and various exogenous drugs such as antifibrotic and anticancer drugs.However,differences in the locations of glycosylation sites,phosphorylation sites,and amino acids in the OAT and OATP structures lead to different substrates being transported to the liver,which ultimately results in their different roles in the liver.To date,few articles have addressed these aspects of OAT and OATP structures,and we study further the similarities and differences in their structures,tissue distribution,substrates,and roles in liver diseases.展开更多
The Na^+/Ca^(2+) exchanger(NCX) protein family is a part of the cation/Ca^(2+) exchanger superfamily and participates in the regulation of cellular Ca^(2+) homeostasis. NCX1, the most important subtype in the NCX fami...The Na^+/Ca^(2+) exchanger(NCX) protein family is a part of the cation/Ca^(2+) exchanger superfamily and participates in the regulation of cellular Ca^(2+) homeostasis. NCX1, the most important subtype in the NCX family, is expressed widely in various organs and tissues in mammals and plays an especially important role in the physiological and pathological processes of nerves and the cardiovascular system. In the past few years, the function of NCX1 in the digestive system has received increasing attention; NCX1 not only participates in the healing process of gastric ulcer and gastric mucosal injury but also mediates the development of digestive cancer, acute pancreatitis, and intestinal absorption.This review aims to explore the roles of NCX1 in digestive system physiology and pathophysiology in order to guide clinical treatments.展开更多
Tic disorder is a common pediatric neurological disorder that severely affects children’s quality of life.But in fact,there is still a lack of a very effective treatment for tic disorder.Acupuncture as a flexible tre...Tic disorder is a common pediatric neurological disorder that severely affects children’s quality of life.But in fact,there is still a lack of a very effective treatment for tic disorder.Acupuncture as a flexible treatment time,treatment cycle control,easy to operate treatment method,widely used in the field of neurological disorder,widely praised by clinicians and patients.At the same time,tic disorder belongs to the category of chronic inflammatory convulsion in traditional Chinese medicine,and acupuncture has a good effect of relieving spasticity and fewer adverse reactions,which makes acupuncture have a good application prospect.Now,more and more studies about acupuncture treatment of tic disorder in children have been published.The main methods of treating tic disorder are acupuncture,electroacupuncture,scalp acupuncture and ear acupuncture.The efficacy of acupuncture alone or in combination with other drugs such as tiapride hydrochloride and haloperidol has been shown to be good.The safety of acupuncture is also commendable,and clinical trials showing that the combination of acupuncture and medication can reduce the incidence and severity of adverse drug reactions.According to the existing studies,acupuncture can promote the development and repair of the nervous system,regulate the secretion of neurotransmitters,and reduce the occurrence of local discomfort,which may be the main mechanism of acupuncture treatment of tic disorders.展开更多
Cancer related fatigue(CRF)is one of the most common and painful reactions in cancer patients.Due to the negative effects of cancer itself and long-term treatment,patients often feel continuous burnout,fatigue and pow...Cancer related fatigue(CRF)is one of the most common and painful reactions in cancer patients.Due to the negative effects of cancer itself and long-term treatment,patients often feel continuous burnout,fatigue and powerlessness in both mental and physical aspects.Fatigue related to cancer and cancer treatment will affect the physical and mental health of cancer patients for a long time and continuously,and it is hard to be relieved through rest.Nowadays,most of the researches focus on the clinical manifestations of patients with cancer,such as pain,nausea and vomiting,weight loss and so on.The fatigue feeling of cancer patients is often one of the most easily overlooked pain symptoms in the treatment process.The influence of CRF on cancer patients may be one of the reasons for reducing the survival time of cancer patients.This paper aims to summarize and explore the pathogenesis of cancer-related fatigue,in order to analyze the related factors of cancer-related fatigue from multi-dimensional and multi-angle,and provide reference for its comprehensive treatment in clinic.展开更多
Based on the Traditional Chinese Medicine Inheritance Support System(V2.5),we explored the compatibility rules of moxibustion and auricular point prescriptions in the treatment of urinary retention after anorectal sur...Based on the Traditional Chinese Medicine Inheritance Support System(V2.5),we explored the compatibility rules of moxibustion and auricular point prescriptions in the treatment of urinary retention after anorectal surgery,and further to obtain new prescriptions.Methods:We searched CNKI,Wanfang,VIP and CBM databases for the literature of moxibustion and auricular point therapy for urinary retention after anorectal surgery,collecting complete prescriptions and entering them into the Traditional Chinese Medicine Inheritance Support System(V2.5).The acupoint frequency,association rules,and combination rules were analyzed by data mining method of software integration.Results:126 moxibustion prescriptions were selected,and the acupoints for urinary retention after anorectal surgery in the literature were analyzed,including 10 acupoints with frequency≥6,9 acupoint combinations with frequency≥26,5 acupoints with association rules,6 core acupoint combinations and 3 new prescription.124 auricular point prescriptions were selected,and the relevant acupoints in the literature were analyzed,including 10 acupoints with frequency of use≥18,15 acupoint combinations with frequency≥40,12 acupoint combinations with association rules,6 core acupoint combinations,and 3 new prescriptions.The core points include Qihai(RN6),Sanyinjiao(SP6),Zhongji(RN3),Guanyuan(RN4).The auricular point therapy is more to select the tenderness points and acupoints that have an impact on the urinary system,mainly including“Pangguang”(CO9),“Shenmen”(TF4),“Shen”(CO10),“Sanjiao”(CO17),“Pizhixia”(AT4),“Niaodao”(HX3),“Fei”(CO14),“Jiaogan”(AH6a).Conclusion:Moxibustion for the treatment of urinary retention is mainly based on invigorating“Primordial Qi”and regulating the functions of“Middle Jiao”(refers to the area of the body just below the diaphragm and above the navel that helps produce delicate nutrients).The entropic clustering of complex systems excavated new prescriptions for the treatment of urinary retention after anorectal surgery,providing new ideas for future research and treatment.展开更多
"Tai Chi","Ba Duan Jin",and"Yi Jin Jing"are traditional Chinese exercises which are used for adjuvant treatment of various diseases.The article outlines the application of three exercises..."Tai Chi","Ba Duan Jin",and"Yi Jin Jing"are traditional Chinese exercises which are used for adjuvant treatment of various diseases.The article outlines the application of three exercises in knee osteoarthritis(KOA).Long-term adherence to traditional exercises("Tai Chi",a traditional Chinese exercise therapies;"Ba Duan Jin",one of the main forms of"Qigong";"Yi Jin Jing",a Chinese ancient exercises)can improve muscle control over bones,improve patients’balance and proprioception,reduce the inflammatory reaction of KOA,protect articular cartilage,and significantly improve the pain,stiffness and mobility of patients with KOA.展开更多
Objective:To explore the potential mechanism of hepatotoxicity induced by Nux Vomica through network toxicology.Methods:The active components and targets of Nux Vomica were identified and screened by Traditional Chine...Objective:To explore the potential mechanism of hepatotoxicity induced by Nux Vomica through network toxicology.Methods:The active components and targets of Nux Vomica were identified and screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform Database(TCMSP),literature research,PubChem Database,Swiss Target Prediction database,etc.Genecards,pharmGKB and OMIM databases were used to collect hepatotoxicity related targets,then,cross them with active component targets to obtain potential targets of hepatotoxicity caused by Nux Vomica.A"Nux Vomica-Potential active components-Potential targets-Hepatotoxicity"network was constructed with Cytoscape 3.8.0 software.The String 11.0 database was used to construct the protein-protein interaction(PPI)network of the targets and to screen out the core targets.In addition,Gene Ontology(GO)function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were conducted by R software,and then the obtained pathways directly related to hepatotoxicity were integrated.Results:In this study,37 active components were screened via TCMSP and literature research,468 targets for the active components of Nux Vomica were obtained.There were 533 hepatotoxicity-related targets,73 potential targets for hepatotoxicity caused by Nux Vomica,and 26 potential active components,among which Ferulic acid,Novacine,Icajine,Simiarenol were the key active components for hepatotoxicity caused by Nux Vomica,and JUN,RELA,and STAT3 were the core target proteins of hepatotoxicity caused by Nux Vomica.There were 1859 GO entries(P-value<0.05),including 1709 entries of Biological Process(BP),39 entries of Cellular Component(CC),and 111 entries of Molecular Function(MF).KEGG enrichment analysis revealed 145 pathways(value<0.05),of which PI3K/AKT signaling pathway,HIF-1 signaling pathway,EGFR tyrosine kinase inhibitor resistance were strongly correlated with the hepatotoxicity caused by Nux Vomica.Conclusion:Through network toxicology analysis,it was found that lots of potential components in Nux Vomica may be involved in the activation of the excessive inflammatory response,oxidative stress,and the LPS response through multiple targets and multiple pathways,resulting in the generation of hepatotoxicity.展开更多
Breast cancer is the most common malignant tumor in women,and the current treatment mainly includes surgery,radiotherapy and chemotherapy.With the development of cell biology,molecular biology and immunology,breast ca...Breast cancer is the most common malignant tumor in women,and the current treatment mainly includes surgery,radiotherapy and chemotherapy.With the development of cell biology,molecular biology and immunology,breast cancer has entered the era of immunotherapy.Through multi-level and multi-way regulation of relevant genes and proteins,TCM regulates the immune microenvironment,and has great advantages in inhibiting tumor progression,alleviating surgical complications,improving immune system function,alleviating the damage to the body caused by surgery,radiotherapy and chemotherapy,and reducing the recurrence rate etc,provides ideas for the comprehensive treatment of breast cancer.This paper discusses the understanding of breast cancer in Traditional Chinese medicine,the influence of immune microenvironment on the occurrence and development of breast cancer,and the regulation of immune microenvironment in breast cancer by traditional Chinese medicine,in order to provide theoretical basis for the diagnosis and treatment of breast cancer with traditional Chinese medicine.展开更多
The decline of ovarian reserve is an important cause of female infertility.More and more older women want to be pregnant again,especially because of the liberal birth policy.But the main reason for limiting fertility ...The decline of ovarian reserve is an important cause of female infertility.More and more older women want to be pregnant again,especially because of the liberal birth policy.But the main reason for limiting fertility in older women is the decline of ovarian reserve.At the same time,due to the change of social environment,the incidence of ovarian function decline has a trend of youth.Therefore,in the infertile women,ovarian dysfunction was found to account for a higher proportion.Finding ways to improve ovarian function is especially important for improving female fertility.Modern medicine has no clear treatment for the disease,only some health products such as coenzyme Q10,dehydroepiandrosterone are used to maintain the ovaries.As a simple and convenient non-drug therapy,acupuncture has been widely used in improving the ovarian function of infertile women in recent years,and its effect has been explored by many researchers.Therefore,this article will start from the human body research and the animal experiment two aspects,discusses the acupuncture improvement ovary function.展开更多
Background:To systematically evaluate the efficacy and safety of traditional Chinese medicine in the treatment of refractory gastroesophageal reflux.Methods:PubMed,The Cochrane Library,Embase,Web of Science,China Nati...Background:To systematically evaluate the efficacy and safety of traditional Chinese medicine in the treatment of refractory gastroesophageal reflux.Methods:PubMed,The Cochrane Library,Embase,Web of Science,China National Knowledge Infrastructure,Wanfang Database,China Science and Technology Journal Database and Chinese BioMedical Literature Database were searched for randomized controlled trials of traditional Chinese medicine in the treatment of refractory gastroesophageal reflux from database establishment time to December 2020.After two researchers independently screened the literature,extracted data and evaluated the bias risk included in the study,RevMan5.3 software was used for meta-analysis.Results:A total of 12 randomized controlled trials,were included,including 893 patients.The results of meta-analysis showed that the total effective rate of the treatment group(relative risk=1.28,95%confidence interval(CI)(1.19,1.38),P<0.00001),RGERDQ(refractory gastroesophageal reflux disease)score(mean difference(MD)=−3.35,95%CI(−4.13,−2.57),P<0.00001],acid reflux(acid in the stomach comes out of the mouth)[MD=−0.30,95%CI(−0.45,−0.15),P<0.00001],heartburn(the feeling that the heart is burned)(MD=−0.44,95%CI(−0.60,−0.29),P<0.00001,and retrosternal pain(MD=−0.27,95%CI(−0.44,−0.10),P<0.00001,belching(MD=−0.40,95%CI(−0.57,−0.24),P<0.00001],endoscopic mucosal score(MD=−0.62,95%CI(−0.78,−0.46),P<0.00001],the differences were statistically significant,and the effective rate of mucosal improvement under endoscopy was P=0.93>0.05,with no statistically significant difference.Conclusion:The current evidence shows that traditional Chinese medicine in the treatment of refractory gastroesophageal reflux disease is better than that of western medicine in the total effective rate,relieving acid reflux,heartburn,retrosternal pain and belching symptoms,but it is impossible to judge the improvement of mucosa under endoscope.Due to the limitations of the quality and quantity of included studies,more high-quality studies are needed to confirm the above conclusions.展开更多
Additive manufacturing of aluminum alloys has received significant attention in the aerospace industry;however,achieving sufficient high strength,especially at elevated temperatures,remains challenging.Here,a crack-fr...Additive manufacturing of aluminum alloys has received significant attention in the aerospace industry;however,achieving sufficient high strength,especially at elevated temperatures,remains challenging.Here,a crack-free and near-full dense Al-1Fe-0.6Cu-1.3Zr alloy was fabricated by the laser powder bed fusion(LPBF)technique.The Al-Fe-Cu-Zr alloy exhibits heterogeneous microstructures with two distinct zones.One is the so-called coarse-grain zones(CGZs)with an average grain size of 0.95μm,where(Al,Cu)Fe_(3) nanoparticles precipitate in the Al matrix and Fe and Cu cosegregate at the grain boundaries(GBs).The other is fine-grain zones(FGZs)with an average grain size of 0.45μm,where an Al 3 Zr nanoparti-cle precipitates in each of theα-Al grains(serves as the nuclei),and Fe-rich nanoprecipitates and Fe/Cu cosegregation appear at the GBs.As a result,the LPBF Al-Fe-Cu-Zr alloy,with these unique heteroge-neous structures,displays high strength at both room temperature and elevated temperatures,e.g.,with high yield strengths of 500 MPa at room temperature,and 163 MPa at 573 K,both are higher than those of additive manufactured Al-based alloys reported thus far.It is suggested that the high strength over a wide temperature range of the current LPBF Al alloy is mainly attributed to the combination of the precipitation strengthening mechanism and grain-boundary strengthening mechanism.展开更多
OBJECTIVE: Traditional Chinese medicine (TCM) is regarded as an important treatment for gastric cancer patients, especially for those in advanced stage. To evaluate the effects of TCM treatment on gastric cancer pa...OBJECTIVE: Traditional Chinese medicine (TCM) is regarded as an important treatment for gastric cancer patients, especially for those in advanced stage. To evaluate the effects of TCM treatment on gastric cancer patients, the authors performed a retrospective study to report the result of the integrated treatment of TCM with chemotherapy for stage IV non-surgical gastric cancer. METHODS: In this study, 182 patients with stage IV and non-surgical gastric cancer were retrospectively analyzed to evaluate the effects of TCM integrated with chemotherapy. Among the 182 cases, 88 cases received integrated therapy consisting of TCM and chemotherapy, while 94 cases received chemotherapy alone. The overall survival and Karnofsky performance status (KPS) score were measured as the main outcome. RESULTS: The median overall survival of the integrated therapy group and chemotherapy group were 16.9 and 10.5 months, respectively. The 1-, 3- and 5-year survival rates of integrated therapy group vs. chemotherapy group were 70% vs. 32%, 18% vs. 4%, and 11% vs. 0%, respectively. There was a significant difference between the two groups (x2 = 42.244, P 〈 0.001 ). After six-month treatment, KPS scores of the integrated therapy group and the chemotherapy group were 75.00 ± 14.78 and 60.64 ± 21.39, respectively (P 〈 0.001). The Cox regression analysis showed that TCM treatment is a protective factor for patients' overall survival. CONCLUSION: This study demonstrated that TCM integrated with chemotherapy may prolong overal survival and improve survival rate and life quality of patients with stage IV non-surgical gastric cancer展开更多
Objectives: Ziyin Huatan Recipe(ZYHT), a traditional Chinese medicine comprised of Lilii Bulbus, Pinelliae Rhizoma, and Hedyotis Diffusa, has shown promise in treating gastric cancer(GC). However, its potential mechan...Objectives: Ziyin Huatan Recipe(ZYHT), a traditional Chinese medicine comprised of Lilii Bulbus, Pinelliae Rhizoma, and Hedyotis Diffusa, has shown promise in treating gastric cancer(GC). However, its potential mechanism has not yet been clearly addressed. This study aimed to predict targets and molecular mechanisms of ZYHT in treating GC by network pharmacology analysis and to explore the role of ZYHT in GC both in vitro and in vivo.Methods: Targets and molecular mechanisms of ZYHT were predicted via network pharmacology analysis. The effects of ZYHT on the expression of metastasis-associated targets were further validated by Western blot and quantitative real-time polymerase chain reaction. To explore the specific molecular mechanisms of the effects of ZYHT on migration and invasion, the runt-related transcription factor 3(RUNX3) gene was knocked out by clustered regularly interspaced short palindromic repeats/Cas9, and lentiviral vectors were transfected into SGC-7901 cells. Then lung metastasis model of GC in nude mice was established to explore the anti-metastasis effect of ZYHT. Western blot and immunohistochemistry were used to explore the impact of ZYHT on the expression of metastasis-related proteins with or without RUNX3 gene.Results: The network pharmacology analysis showed that ZYHT might inhibit focal adhesion, migration,invasion and metastasis of GC. ZYHT inhibited the proliferation, migration and invasion of GC cells in vitro via regulating the expression of metastasis-associated targets. Knocking out RUNX3 almost completely reversed the cell phenotypes(migration and invasion) and protein expression levels elicited by ZYHT.In vivo studies showed that ZYHT inhibited the metastasis of GC cells to the lung and prolonged the survival time of the nude mice. Knocking out RUNX3 partly reversed the metastasis of GC cells to the lung and the protein expression levels elicited by ZYHT.Conclusion: ZYHT can effectively inhibit the invasion and migration of GC in vitro and in vivo, and its molecular mechanism may relate to the upregulation of RUNX3 expression.展开更多
Identification of cancer driver genes plays an important role in precision oncology research,which is helpful to understand cancer initiation and progression.However,most existing computational methods mainly used the...Identification of cancer driver genes plays an important role in precision oncology research,which is helpful to understand cancer initiation and progression.However,most existing computational methods mainly used the protein–protein interaction(PPI)networks,or treated the directed gene regulatory networks(GRNs)as the undirected gene–gene association networks to identify the cancer driver genes,which will lose the unique structure regulatory information in the directed GRNs,and then affect the outcome of the cancer driver gene identification.Here,based on the multi-omics pan-cancer data(i.e.,gene expression,mutation,copy number variation,and DNA methylation),we propose a novel method(called DGMP)to identify cancer driver genes by jointing directed graph convolutional network(DGCN)and multilayer perceptron(MLP).DGMP learns the multi-omics features of genes as well as the topological structure features in GRN with the DGCN model and uses MLP to weigh more on gene features for mitigating the bias toward the graph topological features in the DGCN learning process.The results on three GRNs show that DGMP outperforms other existing state-of-the-art methods.The ablation experimental results on the Dawn Net network indicate that introducing MLP into DGCN can offset the performance degradation of DGCN,and jointing MLP and DGCN can effectively improve the performance of identifying cancer driver genes.DGMP can identify not only the highly mutated cancer driver genes but also the driver genes harboring other kinds of alterations(e.g.,differential expression and aberrant DNA methylation)or genes involved in GRNs with other cancer genes.The source code of DGMP can be freely downloaded from https://github.com/NWPU-903PR/DGMP.展开更多
基金Supported by the National Natural Science Foundation of China,No. 81970541,No. 31960151,No. 81660099 and No. 81660412。
文摘The gut-brain axis is a bidirectional information interaction system between the central nervous system(CNS) and the gastrointestinal tract, in which gut microbiota plays a key role. The gut microbiota forms a complex network with the enteric nervous system, the autonomic nervous system, and the neuroendocrine and neuroimmunity of the CNS, which is called the microbiota-gut-brain axis. Due to the close anatomical and functional interaction of the gut-liver axis, the microbiota-gut-liver-brain axis has attracted increased attention in recent years. The microbiota-gut-liver-brain axis mediates the occurrence and development of many diseases, and it offers a direction for the research of disease treatment. In this review, we mainly discuss the role of the gut microbiota in the irritable bowel syndrome, inflammatory bowel disease, functional dyspepsia, non-alcoholic fatty liver disease, alcoholic liver disease, cirrhosis and hepatic encephalopathy via the gut-liver-brain axis, and the focus is to clarify the potential mechanisms and treatment of digestive diseases based on the further understanding of the microbiota-gut-liver-brain axis.
文摘Organic anion transporters(OATs)and organic anion transporter polypeptides(OATPs)are classified within two SLC superfamilies,namely,the SLC22A superfamily and the SLCO superfamily(formerly the SLC21A family),respectively.They are expressed in many tissues,such as the liver and kidney,and mediate the absorption and excretion of many endogenous and exogenous substances,including various drugs.Most are composed of 12 transmembrane polypeptide chains with the C-terminus and the N-terminus located in the cell cytoplasm.OATs and OATPs are abundantly expressed in the liver,where they mainly promote the uptake of various endogenous substrates such as bile acids and various exogenous drugs such as antifibrotic and anticancer drugs.However,differences in the locations of glycosylation sites,phosphorylation sites,and amino acids in the OAT and OATP structures lead to different substrates being transported to the liver,which ultimately results in their different roles in the liver.To date,few articles have addressed these aspects of OAT and OATP structures,and we study further the similarities and differences in their structures,tissue distribution,substrates,and roles in liver diseases.
基金Supported by the National Natural Science Foundation of China,No.816660412 to Xie R and No.81160265 to Xu JY
文摘The Na^+/Ca^(2+) exchanger(NCX) protein family is a part of the cation/Ca^(2+) exchanger superfamily and participates in the regulation of cellular Ca^(2+) homeostasis. NCX1, the most important subtype in the NCX family, is expressed widely in various organs and tissues in mammals and plays an especially important role in the physiological and pathological processes of nerves and the cardiovascular system. In the past few years, the function of NCX1 in the digestive system has received increasing attention; NCX1 not only participates in the healing process of gastric ulcer and gastric mucosal injury but also mediates the development of digestive cancer, acute pancreatitis, and intestinal absorption.This review aims to explore the roles of NCX1 in digestive system physiology and pathophysiology in order to guide clinical treatments.
文摘Tic disorder is a common pediatric neurological disorder that severely affects children’s quality of life.But in fact,there is still a lack of a very effective treatment for tic disorder.Acupuncture as a flexible treatment time,treatment cycle control,easy to operate treatment method,widely used in the field of neurological disorder,widely praised by clinicians and patients.At the same time,tic disorder belongs to the category of chronic inflammatory convulsion in traditional Chinese medicine,and acupuncture has a good effect of relieving spasticity and fewer adverse reactions,which makes acupuncture have a good application prospect.Now,more and more studies about acupuncture treatment of tic disorder in children have been published.The main methods of treating tic disorder are acupuncture,electroacupuncture,scalp acupuncture and ear acupuncture.The efficacy of acupuncture alone or in combination with other drugs such as tiapride hydrochloride and haloperidol has been shown to be good.The safety of acupuncture is also commendable,and clinical trials showing that the combination of acupuncture and medication can reduce the incidence and severity of adverse drug reactions.According to the existing studies,acupuncture can promote the development and repair of the nervous system,regulate the secretion of neurotransmitters,and reduce the occurrence of local discomfort,which may be the main mechanism of acupuncture treatment of tic disorders.
文摘Cancer related fatigue(CRF)is one of the most common and painful reactions in cancer patients.Due to the negative effects of cancer itself and long-term treatment,patients often feel continuous burnout,fatigue and powerlessness in both mental and physical aspects.Fatigue related to cancer and cancer treatment will affect the physical and mental health of cancer patients for a long time and continuously,and it is hard to be relieved through rest.Nowadays,most of the researches focus on the clinical manifestations of patients with cancer,such as pain,nausea and vomiting,weight loss and so on.The fatigue feeling of cancer patients is often one of the most easily overlooked pain symptoms in the treatment process.The influence of CRF on cancer patients may be one of the reasons for reducing the survival time of cancer patients.This paper aims to summarize and explore the pathogenesis of cancer-related fatigue,in order to analyze the related factors of cancer-related fatigue from multi-dimensional and multi-angle,and provide reference for its comprehensive treatment in clinic.
文摘Based on the Traditional Chinese Medicine Inheritance Support System(V2.5),we explored the compatibility rules of moxibustion and auricular point prescriptions in the treatment of urinary retention after anorectal surgery,and further to obtain new prescriptions.Methods:We searched CNKI,Wanfang,VIP and CBM databases for the literature of moxibustion and auricular point therapy for urinary retention after anorectal surgery,collecting complete prescriptions and entering them into the Traditional Chinese Medicine Inheritance Support System(V2.5).The acupoint frequency,association rules,and combination rules were analyzed by data mining method of software integration.Results:126 moxibustion prescriptions were selected,and the acupoints for urinary retention after anorectal surgery in the literature were analyzed,including 10 acupoints with frequency≥6,9 acupoint combinations with frequency≥26,5 acupoints with association rules,6 core acupoint combinations and 3 new prescription.124 auricular point prescriptions were selected,and the relevant acupoints in the literature were analyzed,including 10 acupoints with frequency of use≥18,15 acupoint combinations with frequency≥40,12 acupoint combinations with association rules,6 core acupoint combinations,and 3 new prescriptions.The core points include Qihai(RN6),Sanyinjiao(SP6),Zhongji(RN3),Guanyuan(RN4).The auricular point therapy is more to select the tenderness points and acupoints that have an impact on the urinary system,mainly including“Pangguang”(CO9),“Shenmen”(TF4),“Shen”(CO10),“Sanjiao”(CO17),“Pizhixia”(AT4),“Niaodao”(HX3),“Fei”(CO14),“Jiaogan”(AH6a).Conclusion:Moxibustion for the treatment of urinary retention is mainly based on invigorating“Primordial Qi”and regulating the functions of“Middle Jiao”(refers to the area of the body just below the diaphragm and above the navel that helps produce delicate nutrients).The entropic clustering of complex systems excavated new prescriptions for the treatment of urinary retention after anorectal surgery,providing new ideas for future research and treatment.
文摘"Tai Chi","Ba Duan Jin",and"Yi Jin Jing"are traditional Chinese exercises which are used for adjuvant treatment of various diseases.The article outlines the application of three exercises in knee osteoarthritis(KOA).Long-term adherence to traditional exercises("Tai Chi",a traditional Chinese exercise therapies;"Ba Duan Jin",one of the main forms of"Qigong";"Yi Jin Jing",a Chinese ancient exercises)can improve muscle control over bones,improve patients’balance and proprioception,reduce the inflammatory reaction of KOA,protect articular cartilage,and significantly improve the pain,stiffness and mobility of patients with KOA.
文摘Objective:To explore the potential mechanism of hepatotoxicity induced by Nux Vomica through network toxicology.Methods:The active components and targets of Nux Vomica were identified and screened by Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform Database(TCMSP),literature research,PubChem Database,Swiss Target Prediction database,etc.Genecards,pharmGKB and OMIM databases were used to collect hepatotoxicity related targets,then,cross them with active component targets to obtain potential targets of hepatotoxicity caused by Nux Vomica.A"Nux Vomica-Potential active components-Potential targets-Hepatotoxicity"network was constructed with Cytoscape 3.8.0 software.The String 11.0 database was used to construct the protein-protein interaction(PPI)network of the targets and to screen out the core targets.In addition,Gene Ontology(GO)function enrichment analysis and Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis were conducted by R software,and then the obtained pathways directly related to hepatotoxicity were integrated.Results:In this study,37 active components were screened via TCMSP and literature research,468 targets for the active components of Nux Vomica were obtained.There were 533 hepatotoxicity-related targets,73 potential targets for hepatotoxicity caused by Nux Vomica,and 26 potential active components,among which Ferulic acid,Novacine,Icajine,Simiarenol were the key active components for hepatotoxicity caused by Nux Vomica,and JUN,RELA,and STAT3 were the core target proteins of hepatotoxicity caused by Nux Vomica.There were 1859 GO entries(P-value<0.05),including 1709 entries of Biological Process(BP),39 entries of Cellular Component(CC),and 111 entries of Molecular Function(MF).KEGG enrichment analysis revealed 145 pathways(value<0.05),of which PI3K/AKT signaling pathway,HIF-1 signaling pathway,EGFR tyrosine kinase inhibitor resistance were strongly correlated with the hepatotoxicity caused by Nux Vomica.Conclusion:Through network toxicology analysis,it was found that lots of potential components in Nux Vomica may be involved in the activation of the excessive inflammatory response,oxidative stress,and the LPS response through multiple targets and multiple pathways,resulting in the generation of hepatotoxicity.
文摘Breast cancer is the most common malignant tumor in women,and the current treatment mainly includes surgery,radiotherapy and chemotherapy.With the development of cell biology,molecular biology and immunology,breast cancer has entered the era of immunotherapy.Through multi-level and multi-way regulation of relevant genes and proteins,TCM regulates the immune microenvironment,and has great advantages in inhibiting tumor progression,alleviating surgical complications,improving immune system function,alleviating the damage to the body caused by surgery,radiotherapy and chemotherapy,and reducing the recurrence rate etc,provides ideas for the comprehensive treatment of breast cancer.This paper discusses the understanding of breast cancer in Traditional Chinese medicine,the influence of immune microenvironment on the occurrence and development of breast cancer,and the regulation of immune microenvironment in breast cancer by traditional Chinese medicine,in order to provide theoretical basis for the diagnosis and treatment of breast cancer with traditional Chinese medicine.
文摘The decline of ovarian reserve is an important cause of female infertility.More and more older women want to be pregnant again,especially because of the liberal birth policy.But the main reason for limiting fertility in older women is the decline of ovarian reserve.At the same time,due to the change of social environment,the incidence of ovarian function decline has a trend of youth.Therefore,in the infertile women,ovarian dysfunction was found to account for a higher proportion.Finding ways to improve ovarian function is especially important for improving female fertility.Modern medicine has no clear treatment for the disease,only some health products such as coenzyme Q10,dehydroepiandrosterone are used to maintain the ovaries.As a simple and convenient non-drug therapy,acupuncture has been widely used in improving the ovarian function of infertile women in recent years,and its effect has been explored by many researchers.Therefore,this article will start from the human body research and the animal experiment two aspects,discusses the acupuncture improvement ovary function.
文摘Background:To systematically evaluate the efficacy and safety of traditional Chinese medicine in the treatment of refractory gastroesophageal reflux.Methods:PubMed,The Cochrane Library,Embase,Web of Science,China National Knowledge Infrastructure,Wanfang Database,China Science and Technology Journal Database and Chinese BioMedical Literature Database were searched for randomized controlled trials of traditional Chinese medicine in the treatment of refractory gastroesophageal reflux from database establishment time to December 2020.After two researchers independently screened the literature,extracted data and evaluated the bias risk included in the study,RevMan5.3 software was used for meta-analysis.Results:A total of 12 randomized controlled trials,were included,including 893 patients.The results of meta-analysis showed that the total effective rate of the treatment group(relative risk=1.28,95%confidence interval(CI)(1.19,1.38),P<0.00001),RGERDQ(refractory gastroesophageal reflux disease)score(mean difference(MD)=−3.35,95%CI(−4.13,−2.57),P<0.00001],acid reflux(acid in the stomach comes out of the mouth)[MD=−0.30,95%CI(−0.45,−0.15),P<0.00001],heartburn(the feeling that the heart is burned)(MD=−0.44,95%CI(−0.60,−0.29),P<0.00001,and retrosternal pain(MD=−0.27,95%CI(−0.44,−0.10),P<0.00001,belching(MD=−0.40,95%CI(−0.57,−0.24),P<0.00001],endoscopic mucosal score(MD=−0.62,95%CI(−0.78,−0.46),P<0.00001],the differences were statistically significant,and the effective rate of mucosal improvement under endoscopy was P=0.93>0.05,with no statistically significant difference.Conclusion:The current evidence shows that traditional Chinese medicine in the treatment of refractory gastroesophageal reflux disease is better than that of western medicine in the total effective rate,relieving acid reflux,heartburn,retrosternal pain and belching symptoms,but it is impossible to judge the improvement of mucosa under endoscope.Due to the limitations of the quality and quantity of included studies,more high-quality studies are needed to confirm the above conclusions.
基金This work was financially supported by the National Natural Science Foundation of China(Nos.52061160483 and 92166130)the Hubei Science Fund for Distinguished Young Scholars(No.2020CFA086).The authors are also grateful to the Analytical and Testing Center,Huazhong University of Science and Technology for technical assistance.
文摘Additive manufacturing of aluminum alloys has received significant attention in the aerospace industry;however,achieving sufficient high strength,especially at elevated temperatures,remains challenging.Here,a crack-free and near-full dense Al-1Fe-0.6Cu-1.3Zr alloy was fabricated by the laser powder bed fusion(LPBF)technique.The Al-Fe-Cu-Zr alloy exhibits heterogeneous microstructures with two distinct zones.One is the so-called coarse-grain zones(CGZs)with an average grain size of 0.95μm,where(Al,Cu)Fe_(3) nanoparticles precipitate in the Al matrix and Fe and Cu cosegregate at the grain boundaries(GBs).The other is fine-grain zones(FGZs)with an average grain size of 0.45μm,where an Al 3 Zr nanoparti-cle precipitates in each of theα-Al grains(serves as the nuclei),and Fe-rich nanoprecipitates and Fe/Cu cosegregation appear at the GBs.As a result,the LPBF Al-Fe-Cu-Zr alloy,with these unique heteroge-neous structures,displays high strength at both room temperature and elevated temperatures,e.g.,with high yield strengths of 500 MPa at room temperature,and 163 MPa at 573 K,both are higher than those of additive manufactured Al-based alloys reported thus far.It is suggested that the high strength over a wide temperature range of the current LPBF Al alloy is mainly attributed to the combination of the precipitation strengthening mechanism and grain-boundary strengthening mechanism.
基金supported by the Natural Science Foundation of Shanghai Science and Technology Commission (No. 17ZR1438800, for Dr. Xuan Liu)Traditional Chinese Medicine Guiding Project of Shanghai Science and Technology Commission (No . 13401907500 , for Zhi-feng Qin)+1 种基金Shanghai Municipal Commission of Health and Family Planning Program (No. ZY3-CCCX-3-3056, for Dr. Li-juan Xiu)National Natural Science Foundation Youth Program (No. 81603434, for Dr. Li-juan Xiu)
文摘OBJECTIVE: Traditional Chinese medicine (TCM) is regarded as an important treatment for gastric cancer patients, especially for those in advanced stage. To evaluate the effects of TCM treatment on gastric cancer patients, the authors performed a retrospective study to report the result of the integrated treatment of TCM with chemotherapy for stage IV non-surgical gastric cancer. METHODS: In this study, 182 patients with stage IV and non-surgical gastric cancer were retrospectively analyzed to evaluate the effects of TCM integrated with chemotherapy. Among the 182 cases, 88 cases received integrated therapy consisting of TCM and chemotherapy, while 94 cases received chemotherapy alone. The overall survival and Karnofsky performance status (KPS) score were measured as the main outcome. RESULTS: The median overall survival of the integrated therapy group and chemotherapy group were 16.9 and 10.5 months, respectively. The 1-, 3- and 5-year survival rates of integrated therapy group vs. chemotherapy group were 70% vs. 32%, 18% vs. 4%, and 11% vs. 0%, respectively. There was a significant difference between the two groups (x2 = 42.244, P 〈 0.001 ). After six-month treatment, KPS scores of the integrated therapy group and the chemotherapy group were 75.00 ± 14.78 and 60.64 ± 21.39, respectively (P 〈 0.001). The Cox regression analysis showed that TCM treatment is a protective factor for patients' overall survival. CONCLUSION: This study demonstrated that TCM integrated with chemotherapy may prolong overal survival and improve survival rate and life quality of patients with stage IV non-surgical gastric cancer
基金supported by National Natural Science Foundation of China (No. 81873215 and No. 81804008)Shanghai Science and Technology Fund (No. 19401971700)Science Research Project of Strategic Support Force Xingcheng Special Duty Sanatorium (No. 20YG003 and No. 21YG001)。
文摘Objectives: Ziyin Huatan Recipe(ZYHT), a traditional Chinese medicine comprised of Lilii Bulbus, Pinelliae Rhizoma, and Hedyotis Diffusa, has shown promise in treating gastric cancer(GC). However, its potential mechanism has not yet been clearly addressed. This study aimed to predict targets and molecular mechanisms of ZYHT in treating GC by network pharmacology analysis and to explore the role of ZYHT in GC both in vitro and in vivo.Methods: Targets and molecular mechanisms of ZYHT were predicted via network pharmacology analysis. The effects of ZYHT on the expression of metastasis-associated targets were further validated by Western blot and quantitative real-time polymerase chain reaction. To explore the specific molecular mechanisms of the effects of ZYHT on migration and invasion, the runt-related transcription factor 3(RUNX3) gene was knocked out by clustered regularly interspaced short palindromic repeats/Cas9, and lentiviral vectors were transfected into SGC-7901 cells. Then lung metastasis model of GC in nude mice was established to explore the anti-metastasis effect of ZYHT. Western blot and immunohistochemistry were used to explore the impact of ZYHT on the expression of metastasis-related proteins with or without RUNX3 gene.Results: The network pharmacology analysis showed that ZYHT might inhibit focal adhesion, migration,invasion and metastasis of GC. ZYHT inhibited the proliferation, migration and invasion of GC cells in vitro via regulating the expression of metastasis-associated targets. Knocking out RUNX3 almost completely reversed the cell phenotypes(migration and invasion) and protein expression levels elicited by ZYHT.In vivo studies showed that ZYHT inhibited the metastasis of GC cells to the lung and prolonged the survival time of the nude mice. Knocking out RUNX3 partly reversed the metastasis of GC cells to the lung and the protein expression levels elicited by ZYHT.Conclusion: ZYHT can effectively inhibit the invasion and migration of GC in vitro and in vivo, and its molecular mechanism may relate to the upregulation of RUNX3 expression.
基金supported in part by the National Natural Science Foundation of China(Grant Nos.62173271 and 61873202 to SWZ)。
文摘Identification of cancer driver genes plays an important role in precision oncology research,which is helpful to understand cancer initiation and progression.However,most existing computational methods mainly used the protein–protein interaction(PPI)networks,or treated the directed gene regulatory networks(GRNs)as the undirected gene–gene association networks to identify the cancer driver genes,which will lose the unique structure regulatory information in the directed GRNs,and then affect the outcome of the cancer driver gene identification.Here,based on the multi-omics pan-cancer data(i.e.,gene expression,mutation,copy number variation,and DNA methylation),we propose a novel method(called DGMP)to identify cancer driver genes by jointing directed graph convolutional network(DGCN)and multilayer perceptron(MLP).DGMP learns the multi-omics features of genes as well as the topological structure features in GRN with the DGCN model and uses MLP to weigh more on gene features for mitigating the bias toward the graph topological features in the DGCN learning process.The results on three GRNs show that DGMP outperforms other existing state-of-the-art methods.The ablation experimental results on the Dawn Net network indicate that introducing MLP into DGCN can offset the performance degradation of DGCN,and jointing MLP and DGCN can effectively improve the performance of identifying cancer driver genes.DGMP can identify not only the highly mutated cancer driver genes but also the driver genes harboring other kinds of alterations(e.g.,differential expression and aberrant DNA methylation)or genes involved in GRNs with other cancer genes.The source code of DGMP can be freely downloaded from https://github.com/NWPU-903PR/DGMP.