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Herpes simplex vires thymidine kinase and ganciclovir suicide gene therapy for human pancreatic cancer 被引量:16
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作者 jingwang xiao-xuanlu dao-zhenchen shu-fengli li-shanzhang 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第3期400-403,共4页
AIM:To investigate the in vitro effects of suicide gene therapy system of herpes simplex virus thymidine kinase gene (HSV-TK) in combination with the treatment of nucleotide analog-ganciclovir (GCV) on human pancreati... AIM:To investigate the in vitro effects of suicide gene therapy system of herpes simplex virus thymidine kinase gene (HSV-TK) in combination with the treatment of nucleotide analog-ganciclovir (GCV) on human pancreatic cancer, and to provide a novel clinical therapeutic method for human pancreatic cancer.METHODS: We used a replication defective recombinant retrovirus vector GINaTK (bearing HSV-TK gene) to make packaging cell PA317 produce progeny virions. We then transferred the HSV-TK gene to target cells SW1990 using these progeny virions, and treated these gene-modified tumor cells with GCV to study the sensitivity of the cells to GCV and their bystander effects by routine MTT-method.RESULTS:Packaging cell PA317/TK was successfully constructed, and we acquired SW1990/TK through virus progeny infection. These gene-modified pancreatic cancer cells were sensitive to the treatment of GCV compared with unmodified tumor cells (t=4.15,n=10,P<0.0025). We also observed a remarkable bystander effect by mixing two kinds of cells at different ratio.CONCLUSION:Our data demonstrate that HSV-TK/GCV suicide gene therapy system is effective for treating experimental human pancreatic cancer, which is largely resistant to the common therapies,so the suicide gene therapy system may be a potential treatment approach for pancreatic cancer. 展开更多
关键词 胸腺密啶核苷激酶 单纯疱疹病毒 自杀基因 基因疗法 胰腺癌 MTT法
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