Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque.It remains challenges for current practice to specifically identify,interven...Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque.It remains challenges for current practice to specifically identify,intervene and interrupt the development of caries while restoring defects.In this study,inspired by natural dental plaque,a stimuli-responsive multidrug delivery system(PMs@NaF-SAP)has been developed to prevent tooth decay and promote enamel restoration.Classic spherical core-shell structures of micelles dual-loaded with antibacterial and restorative agents are self-assembled into bacteria-responsive multidrug delivery system based on the pH-cleavable boronate ester bond,followed by conjugation with salivary-acquired peptide(SAP)to endow the nanoparticle with strong adhesion to tooth enamel.The constructed PMs@NaF-SAP specifically adheres to tooth,identifies cariogenic conditions and intelligently releases drugs at acidic pH,thereby providing antibacterial adhesion and cariogenic biofilm resistance,and restoring the microarchitecture and mechanical properties of demineralized teeth.Topical treatment with PMs@NaF-SAP effectively diminishes the onset and severity of caries without impacting oral microbiota diversity or surrounding mucosal tissues.These findings demonstrate this novel nanotherapy has potential as a promising biomedical application for caries prevention and tooth defect restoration while resisting biofilm-associated diseases in a controlled manner activated by pathological bacteria.展开更多
As a global public health focus,oral health plays a vital role in facilitating overall health.Defected teeth characterized by exposure of dentin generally increase the risk of aggravating oral diseases.The exposed den...As a global public health focus,oral health plays a vital role in facilitating overall health.Defected teeth characterized by exposure of dentin generally increase the risk of aggravating oral diseases.The exposed dentinal tubules provide channels for irritants and bacterial invasion,leading to dentin hypersensitivity and even pulp inflammation.Cariogenic bacterial adhesion and biofilm formation on dentin are responsible for tooth demineralization and caries.It remains a clinical challenge to achieve the integration of tubule occlusion,collagen mineralization,and antibiofilm functions for managing exposed dentin.To address this issue,an epigallocatechin-3-gallate(EGCG)and poly(allylamine)-stabilized amorphous calcium phosphate(PAH-ACP)co-delivery hollow mesoporous silica(HMS)nanosystem(E/PA@HMS)was herein developed.The application of E/PA@HMS effectively occluded the dentinal tubules with acid-and abrasion-resistant stability and inhibited the biofilm formation of Streptococcus mutans.Intrafibrillar mineralization of collagen fibrils and remineralization of demineralized dentin were induced by E/PA@HMS.The odontogenic differentiation and mineralization of dental pulp cells with high biocompatibility were also promoted.Animal experiments showed that E/PA@HMS durably sealed the tubules and inhibited biofilm growth up to 14 days.Thus,the development of the E/PA@HMS nanosystem provides promising benefits for protecting exposed dentin through the coordinated manipulation of dentin caries and hypersensitivity.展开更多
The authors regret the incorrect publication of Fig.5,which was not identified during the proofing stage.During a self-check,we unfortunately found that the insets(250μg/mL,1 day)in Fig.5b/c and the image(1000μg/mL,...The authors regret the incorrect publication of Fig.5,which was not identified during the proofing stage.During a self-check,we unfortunately found that the insets(250μg/mL,1 day)in Fig.5b/c and the image(1000μg/mL,3 days)in Fig.5c were mistakenly used during figure assembly.We tracked down the original data obtained in June 2022,and have replaced Fig.5 with the correct image as follows.The authors would like to apologise for any inconvenience caused and state that the correction does not change the scientific conclusions of the article in any way.展开更多
基金supported by the National Natural Science Foundation of China(No.82001106,81970918,81901043).
文摘Dental caries is one of the most prevalent human diseases resulting from tooth demineralization caused by acid production of bacteria plaque.It remains challenges for current practice to specifically identify,intervene and interrupt the development of caries while restoring defects.In this study,inspired by natural dental plaque,a stimuli-responsive multidrug delivery system(PMs@NaF-SAP)has been developed to prevent tooth decay and promote enamel restoration.Classic spherical core-shell structures of micelles dual-loaded with antibacterial and restorative agents are self-assembled into bacteria-responsive multidrug delivery system based on the pH-cleavable boronate ester bond,followed by conjugation with salivary-acquired peptide(SAP)to endow the nanoparticle with strong adhesion to tooth enamel.The constructed PMs@NaF-SAP specifically adheres to tooth,identifies cariogenic conditions and intelligently releases drugs at acidic pH,thereby providing antibacterial adhesion and cariogenic biofilm resistance,and restoring the microarchitecture and mechanical properties of demineralized teeth.Topical treatment with PMs@NaF-SAP effectively diminishes the onset and severity of caries without impacting oral microbiota diversity or surrounding mucosal tissues.These findings demonstrate this novel nanotherapy has potential as a promising biomedical application for caries prevention and tooth defect restoration while resisting biofilm-associated diseases in a controlled manner activated by pathological bacteria.
基金This work was financially supported by National Natural Science Foundation of China(81901043,81970918,and 82001106)J.Yu’s research conducted at the UBC Faculty of Dentistry was supported by China Scholarship Council(202006275049).
文摘As a global public health focus,oral health plays a vital role in facilitating overall health.Defected teeth characterized by exposure of dentin generally increase the risk of aggravating oral diseases.The exposed dentinal tubules provide channels for irritants and bacterial invasion,leading to dentin hypersensitivity and even pulp inflammation.Cariogenic bacterial adhesion and biofilm formation on dentin are responsible for tooth demineralization and caries.It remains a clinical challenge to achieve the integration of tubule occlusion,collagen mineralization,and antibiofilm functions for managing exposed dentin.To address this issue,an epigallocatechin-3-gallate(EGCG)and poly(allylamine)-stabilized amorphous calcium phosphate(PAH-ACP)co-delivery hollow mesoporous silica(HMS)nanosystem(E/PA@HMS)was herein developed.The application of E/PA@HMS effectively occluded the dentinal tubules with acid-and abrasion-resistant stability and inhibited the biofilm formation of Streptococcus mutans.Intrafibrillar mineralization of collagen fibrils and remineralization of demineralized dentin were induced by E/PA@HMS.The odontogenic differentiation and mineralization of dental pulp cells with high biocompatibility were also promoted.Animal experiments showed that E/PA@HMS durably sealed the tubules and inhibited biofilm growth up to 14 days.Thus,the development of the E/PA@HMS nanosystem provides promising benefits for protecting exposed dentin through the coordinated manipulation of dentin caries and hypersensitivity.
文摘The authors regret the incorrect publication of Fig.5,which was not identified during the proofing stage.During a self-check,we unfortunately found that the insets(250μg/mL,1 day)in Fig.5b/c and the image(1000μg/mL,3 days)in Fig.5c were mistakenly used during figure assembly.We tracked down the original data obtained in June 2022,and have replaced Fig.5 with the correct image as follows.The authors would like to apologise for any inconvenience caused and state that the correction does not change the scientific conclusions of the article in any way.
