We report a complete genomic sequence of rare isolates (minor genotype) of theSARS-CoV from SARS patients in Guangdong, China, where the first few casesemerged. The most striking discovery from the isolate is an extra...We report a complete genomic sequence of rare isolates (minor genotype) of theSARS-CoV from SARS patients in Guangdong, China, where the first few casesemerged. The most striking discovery from the isolate is an extra 29-nucleotidesequence located at the nucleotide positions between 27,863 and 27,864 (referredto the complete sequence of B J01) within an overlapped region composed of BGI-PUP5 (BGI-postulated uncharacterized protein 5) and BGI-PUP6 upstream ofthe N (nucleocapsid) protein. The discovery of this minor genotype, GD-Ins29,suggests a significant genetic event and differentiates it from the previously re-ported genotype, the dominant form among all sequenced SARS-CoV isolates. A17-nt segment of this extra sequence is identical to a segment of the same size intwo human mRNA sequences that may interfere with viral genome replication andtranscription in the cytosol of the infected cells. It provides a new avenue for theexploration of the virus-host interaction in viral evolution, host pathogenesis, andvaccine development.展开更多
The Coronaviridae family is characterized by a nucleocapsid that is composed of thegenome RNA molecule in combination with the nucleoprotein (N protein) withina virion. The most striking physiochemical feature of the ...The Coronaviridae family is characterized by a nucleocapsid that is composed of thegenome RNA molecule in combination with the nucleoprotein (N protein) withina virion. The most striking physiochemical feature of the N protein of SARS-CoVis that it is a typical basic protein with a high predicted pI and high hydrophilicity,which is consistent with its function of binding to the ribophosphate backbone ofthe RNA molecule. The predicted high extent of phosphorylation of the N proteinon multiple candidate phosphorylation sites demonstrates that it would be relatedto important functions, such as RNA-binding and localization to the nucleolus ofhost cells. Subsequent study shows that there is an SR-rich region in the N proteinand this region might be involved in the protein-protein interaction. The abundantantigenic sites predicted in the N protein, as well as experimental evidence withsynthesized polypeptides, indicate that the N protein is one of the major antigensof the SARS-CoV. Compared with other viral structural proteins, the low variationrate of the N protein with regards to its size suggests its importance to the survivalof the virus.展开更多
文摘We report a complete genomic sequence of rare isolates (minor genotype) of theSARS-CoV from SARS patients in Guangdong, China, where the first few casesemerged. The most striking discovery from the isolate is an extra 29-nucleotidesequence located at the nucleotide positions between 27,863 and 27,864 (referredto the complete sequence of B J01) within an overlapped region composed of BGI-PUP5 (BGI-postulated uncharacterized protein 5) and BGI-PUP6 upstream ofthe N (nucleocapsid) protein. The discovery of this minor genotype, GD-Ins29,suggests a significant genetic event and differentiates it from the previously re-ported genotype, the dominant form among all sequenced SARS-CoV isolates. A17-nt segment of this extra sequence is identical to a segment of the same size intwo human mRNA sequences that may interfere with viral genome replication andtranscription in the cytosol of the infected cells. It provides a new avenue for theexploration of the virus-host interaction in viral evolution, host pathogenesis, andvaccine development.
文摘The Coronaviridae family is characterized by a nucleocapsid that is composed of thegenome RNA molecule in combination with the nucleoprotein (N protein) withina virion. The most striking physiochemical feature of the N protein of SARS-CoVis that it is a typical basic protein with a high predicted pI and high hydrophilicity,which is consistent with its function of binding to the ribophosphate backbone ofthe RNA molecule. The predicted high extent of phosphorylation of the N proteinon multiple candidate phosphorylation sites demonstrates that it would be relatedto important functions, such as RNA-binding and localization to the nucleolus ofhost cells. Subsequent study shows that there is an SR-rich region in the N proteinand this region might be involved in the protein-protein interaction. The abundantantigenic sites predicted in the N protein, as well as experimental evidence withsynthesized polypeptides, indicate that the N protein is one of the major antigensof the SARS-CoV. Compared with other viral structural proteins, the low variationrate of the N protein with regards to its size suggests its importance to the survivalof the virus.
