N6-Methyladenine (6mA)DNA methylation has recently been implicated as a potential new epigenetic marker in eukaryotes,including the dioot modelArabidopsis thaliana.However,the conservation and divergence of 6mA distri...N6-Methyladenine (6mA)DNA methylation has recently been implicated as a potential new epigenetic marker in eukaryotes,including the dioot modelArabidopsis thaliana.However,the conservation and divergence of 6mA distribution patterns and functions in plants remain elusive.Here we report high-quality 6mA methylomes at single-nucleotide resolution in rice based on substantially improved genome sequences of two rice cultivars,Nipponbare (Nip;Japonica)and 93-11 (Indica).Analysis of 6mA genomic distribution and its association with transcription suggest that 6mA distribution and function is rather conserved between rice and Arabidopsis.We found that 6mA levels are positively correlated with the expression of key stressrelated genes,which may be responsible for the difference in stress tolerance between Nip and 93-11. Moreover,we showed that mutations in DDM1 cause defects in plant growth and decreased 6mA level. Our results reveal that 6mA is a conserved DNA modification that is positively associated with gene expression and contributes to key agronomic traits in plants.展开更多
The most common and abundant DNA modification is 5-methylcytosine（5mC）,which has been well-established as an epigenetic mark regulating gene expression in eukaryotes（Jones,2012）.Another DNA modification N^6-methyl...The most common and abundant DNA modification is 5-methylcytosine（5mC）,which has been well-established as an epigenetic mark regulating gene expression in eukaryotes（Jones,2012）.Another DNA modification N^6-methyldeoxyadenosine（6mA）,previously reported as a widespread DNA methylation in prokaryotes.展开更多
Nitrogen-containing polymers have found a wide myriad of applications in materials science and other different areas,and have attracted much attention towards the development of new synthetic methods.Ring-opening meta...Nitrogen-containing polymers have found a wide myriad of applications in materials science and other different areas,and have attracted much attention towards the development of new synthetic methods.Ring-opening metathesis polymerization represents an area of great versatility and potential for the synthesis of highly functionalized polymers with low dispersity and control over molecular weight and architecture complexity.Expanding the scope of monomers through the incorporation of nitrogen atoms would further expand their utilities.In this work,a controlled synthesis of polymers with in-chain enamide moiety through ring-opening metathesis polymerization was developed by utilizing azetine derivatives as the monomers.Relatively short synthetic routes were designed and generated a panel of five monomers.The polymerization exhibited living characteristics,as linear relationship between molecular weights and degrees of polymerization and relatively narrow molar mass distributions were observed,which was further confirmed by successful diblock copolymers formation through sequential addition of two monomers.The resulting polymers that contain the enamide moiety within the backbone exhibited good stability and underwent facile degradation under acidic conditions.展开更多
基金The Ministry of Science and Technology of the People's Republic of China (2016YFD0101001)to X.G.,National Natural Science Foundation of China (31871606,31671670)to X.G.,Recruitment program of Global Youth Expert of China to X.G.,and Fundamental Research funds for Central Non-profit Scientific Institution (1610392017001)to X.G.
文摘N6-Methyladenine (6mA)DNA methylation has recently been implicated as a potential new epigenetic marker in eukaryotes,including the dioot modelArabidopsis thaliana.However,the conservation and divergence of 6mA distribution patterns and functions in plants remain elusive.Here we report high-quality 6mA methylomes at single-nucleotide resolution in rice based on substantially improved genome sequences of two rice cultivars,Nipponbare (Nip;Japonica)and 93-11 (Indica).Analysis of 6mA genomic distribution and its association with transcription suggest that 6mA distribution and function is rather conserved between rice and Arabidopsis.We found that 6mA levels are positively correlated with the expression of key stressrelated genes,which may be responsible for the difference in stress tolerance between Nip and 93-11. Moreover,we showed that mutations in DDM1 cause defects in plant growth and decreased 6mA level. Our results reveal that 6mA is a conserved DNA modification that is positively associated with gene expression and contributes to key agronomic traits in plants.
基金supported by Recruitment program of Global Youth Expert of China (to X.G.)the Elite Youth Program of the Chinese Academy of Agricultural Science (to X.G.)the intramural research support from Biotechnology Research Institute, Chinese Academy of Agricultural Sciences
文摘The most common and abundant DNA modification is 5-methylcytosine（5mC）,which has been well-established as an epigenetic mark regulating gene expression in eukaryotes（Jones,2012）.Another DNA modification N^6-methyldeoxyadenosine（6mA）,previously reported as a widespread DNA methylation in prokaryotes.
基金the National Natural Science Foundation of China(22101087)the Natural Science Foundation of Guangdong Province(2023A1515011714)for the financial support.
文摘Nitrogen-containing polymers have found a wide myriad of applications in materials science and other different areas,and have attracted much attention towards the development of new synthetic methods.Ring-opening metathesis polymerization represents an area of great versatility and potential for the synthesis of highly functionalized polymers with low dispersity and control over molecular weight and architecture complexity.Expanding the scope of monomers through the incorporation of nitrogen atoms would further expand their utilities.In this work,a controlled synthesis of polymers with in-chain enamide moiety through ring-opening metathesis polymerization was developed by utilizing azetine derivatives as the monomers.Relatively short synthetic routes were designed and generated a panel of five monomers.The polymerization exhibited living characteristics,as linear relationship between molecular weights and degrees of polymerization and relatively narrow molar mass distributions were observed,which was further confirmed by successful diblock copolymers formation through sequential addition of two monomers.The resulting polymers that contain the enamide moiety within the backbone exhibited good stability and underwent facile degradation under acidic conditions.
