A simple approach for the preparation of emitter of electrospray ionization mass spectrometry by covalently coating the fused-silica capillary tip with the conductive carbon-nanotube sol-gel composite material(CNTSCM)...A simple approach for the preparation of emitter of electrospray ionization mass spectrometry by covalently coating the fused-silica capillary tip with the conductive carbon-nanotube sol-gel composite material(CNTSCM)is described.The CNTSCM was prepared by dispersing single-walled carbon nanotubes in the sol composed of a mixture of 3-glycidoxypropyltrimethoxysilane and 3-aminopropyltrimethoxysilane,and ethanol.The long-term sta-bility of the prepared ESI emitters was demonstrated by at least 180 h of continuous use.Signal intensity obtained by the prepared emitter was mass-flux sensitive when the flow rate was lower than 500 nL/min,while the signal in-tensity performed a concentration dependence when the flow rate was in the range of 500-800 nL/min.The use-fulness of such a prepared emitter was demonstrated by the analysis of various types of samples such as organic small molecular drugs,oligosaccharide,peptide,and protein.展开更多
of main observation and conclusion We developed a method for comprehensively profiling drug-protein interactions using micro-column affinity purification(AP)combined with label-free quantitative(LFQ)proteomics as well...of main observation and conclusion We developed a method for comprehensively profiling drug-protein interactions using micro-column affinity purification(AP)combined with label-free quantitative(LFQ)proteomics as well as the statistical and bioinformatics analysis.FK506 was used as the experimental model for proof of concept.The true interacting proteins were distinguished from the background proteins by their fold changes of FLQ intensities combined with p-values.Totally 116 FK506 interacting proteins including 5 known target proteins were identified.The method was validated by using the LFQ intensity of the endogenous known drug targets together with statistical analysis.We demonstrated that the micro-column-based affinity purification in combination with LFQ proteomics provides a highly reproducible and robust approach for profiling drug-protein interactions.展开更多
基金This work was financially supported by the National Natural Science Foundations of China(No.21175146).
文摘A simple approach for the preparation of emitter of electrospray ionization mass spectrometry by covalently coating the fused-silica capillary tip with the conductive carbon-nanotube sol-gel composite material(CNTSCM)is described.The CNTSCM was prepared by dispersing single-walled carbon nanotubes in the sol composed of a mixture of 3-glycidoxypropyltrimethoxysilane and 3-aminopropyltrimethoxysilane,and ethanol.The long-term sta-bility of the prepared ESI emitters was demonstrated by at least 180 h of continuous use.Signal intensity obtained by the prepared emitter was mass-flux sensitive when the flow rate was lower than 500 nL/min,while the signal in-tensity performed a concentration dependence when the flow rate was in the range of 500-800 nL/min.The use-fulness of such a prepared emitter was demonstrated by the analysis of various types of samples such as organic small molecular drugs,oligosaccharide,peptide,and protein.
基金This work was financially supported by the National NaturalScience Foundations of China(Nos.21775158,21375140,21175146)the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB20020200).
文摘of main observation and conclusion We developed a method for comprehensively profiling drug-protein interactions using micro-column affinity purification(AP)combined with label-free quantitative(LFQ)proteomics as well as the statistical and bioinformatics analysis.FK506 was used as the experimental model for proof of concept.The true interacting proteins were distinguished from the background proteins by their fold changes of FLQ intensities combined with p-values.Totally 116 FK506 interacting proteins including 5 known target proteins were identified.The method was validated by using the LFQ intensity of the endogenous known drug targets together with statistical analysis.We demonstrated that the micro-column-based affinity purification in combination with LFQ proteomics provides a highly reproducible and robust approach for profiling drug-protein interactions.
