Strawberry‘Kaorino’is one of the perfect early-maturing strawberry varieties with high quality and high yield due to its anthracnose resistance,early maturity and good quality.The variety has become more and more po...Strawberry‘Kaorino’is one of the perfect early-maturing strawberry varieties with high quality and high yield due to its anthracnose resistance,early maturity and good quality.The variety has become more and more popular with the promotion over the last few years,but there are great differences from other varieties in cultivation.Based on its varietal characteristics and cultivation performance in production over the last few years,we summarized the seedling cultivation techniques,planting management techniques,main disease control techniques and harvesting and storage techniques,aiming to provide reference for better promotion and application of‘Kaorino’.展开更多
How to trigger strong anti-tumor immune responses has become a focus for tumor therapy.Here,we report the human-induced pluripotent stem cells(iPSs)to deliver MnO2@Ce6 nanoprobes into tumors for simultaneous photodyna...How to trigger strong anti-tumor immune responses has become a focus for tumor therapy.Here,we report the human-induced pluripotent stem cells(iPSs)to deliver MnO2@Ce6 nanoprobes into tumors for simultaneous photodynamic therapy(PDT)and enhanced immunotherapy.Ce6 photosensitizer was attached on manganese dioxide(MnO2)nanoparticles,and resultant MnO2@Ce6 nanoprobes were delivered into mitomycin-treated iPSs to form iPS-MnO2@Ce6 nanoprobes.The iPS-MnO2@Ce6 actively targeted in vivo tumors,the acidic microenvironment triggered interaction between MnO2 and H2O2,released large quantities of oxygen,alleviated hypoxia in tumor.Upon PDT,singlet oxygen formed,broken iPSs released tumor-shared antigens,which evoked an intensive innate and adaptive immune response against the tumor,improving dendritic cells matured,effector T cells,and natural killer cells were activated.Meanwhile,regulatory T cells were reduced,and then the immune response induced by iPS-MnO2@Ce6 was markedly stronger than the immune reaction induced by MnO2@Ce6(P<0.05).The iPS-MnO2@Ce6 markedly inhibited tumor growth and metastasis and reduced mortality in mice models with tumor.Human iPS s loaded with MnO2-based nanoprobes are a promising strategy for simultaneous PDT and enhanced immunotherapy against tumor and own clinical translational prospect.展开更多
文摘Strawberry‘Kaorino’is one of the perfect early-maturing strawberry varieties with high quality and high yield due to its anthracnose resistance,early maturity and good quality.The variety has become more and more popular with the promotion over the last few years,but there are great differences from other varieties in cultivation.Based on its varietal characteristics and cultivation performance in production over the last few years,we summarized the seedling cultivation techniques,planting management techniques,main disease control techniques and harvesting and storage techniques,aiming to provide reference for better promotion and application of‘Kaorino’.
基金financially supported by National Nature Scientific foundation(81803094,81802979 and 81921002)the National Foundational Basic Research Project of China(2017YFA0205301 and 2015CB931802)+3 种基金Shanghai Municipal Commission of Economy and Information Technology Fund(No.XC-ZXSJ-02-2016-05)the medical engineering cross project of Shanghai Jiao Tong university(YG2017ZD05,YG2016ZD10 and YG2017Z D05)the Project of Thousand Youth Talents from Chinathe National Key Research and Development Program of China(2017YFC1200904 and 2017YFE0124400)。
文摘How to trigger strong anti-tumor immune responses has become a focus for tumor therapy.Here,we report the human-induced pluripotent stem cells(iPSs)to deliver MnO2@Ce6 nanoprobes into tumors for simultaneous photodynamic therapy(PDT)and enhanced immunotherapy.Ce6 photosensitizer was attached on manganese dioxide(MnO2)nanoparticles,and resultant MnO2@Ce6 nanoprobes were delivered into mitomycin-treated iPSs to form iPS-MnO2@Ce6 nanoprobes.The iPS-MnO2@Ce6 actively targeted in vivo tumors,the acidic microenvironment triggered interaction between MnO2 and H2O2,released large quantities of oxygen,alleviated hypoxia in tumor.Upon PDT,singlet oxygen formed,broken iPSs released tumor-shared antigens,which evoked an intensive innate and adaptive immune response against the tumor,improving dendritic cells matured,effector T cells,and natural killer cells were activated.Meanwhile,regulatory T cells were reduced,and then the immune response induced by iPS-MnO2@Ce6 was markedly stronger than the immune reaction induced by MnO2@Ce6(P<0.05).The iPS-MnO2@Ce6 markedly inhibited tumor growth and metastasis and reduced mortality in mice models with tumor.Human iPS s loaded with MnO2-based nanoprobes are a promising strategy for simultaneous PDT and enhanced immunotherapy against tumor and own clinical translational prospect.
