hsa_circ_0007460通过调节巨噬细胞的自噬和凋亡影响细胞内结核分枝杆菌的存活

环状RNA(circular RNA,circRNA)是一类缺乏5′-帽子和3′-poly(A)尾巴的非编码RNA,可以参与多种人类疾病的生物学过程。然而,对其在活动性肺结核(active pulmonary tuberculosis,ATB)中的诊断和功能价值却知之甚少。本研究旨在研究hsa_circ_0007460是否能作为ATB患者的潜在诊断生物标志物,并对其功能进行初探。通过实时荧光定量PCR(real-time quantitative fluorescent PCR,RT-qPCR)发现hsa_circ_0007460在32例ATB患者的外周血以及牛结核分枝杆菌的减毒株——BCG(bacillus Calmette-Guerin)感染的THP-1人源巨噬细胞中显著上调。受试者工作特征曲线(receiver operating curve,ROC)显示曲线下面积(the area under ROC curve,AUC)为0.7474,灵敏度76.67%,特异度78.13%。通过RNase R消化和放线菌素D抑制实验证实hsa_circ_0007460相较于其线性mRNA更加稳定,提示其有作为ATB的诊断生物标志物的潜力。通过蛋白质印迹(Western blot)、CCK-8(cell counting kit-8)、平板计数、免疫荧光等实验表明hsa_circ_0007460能调控巨噬细胞凋亡和自噬。最后,通过生物信息学分析预测下游的miRNA和mRNA,构建了hsa_circ_0007460/hsa-miR-3127-5p/PATZ1轴。上述研究结果表明,hsa_circ_0007460在ATB患者外周血中显著上调,可以作为潜在的诊断生物标志物,且hsa_circ_0007460能促进巨噬细胞凋亡、抑制巨噬细胞自噬从而促进胞内BCG的存活。

Impact of Labor Mobility and College Volunteers' Fixed-position Teaching Experience on Rural Households——Based on Survey Data about Teaching Sites and Volunteer Teachers

With the continuous deepening of reform and opening up,the gap between urban and rural areas continues to widen,and many farmers in rural areas have chosen to work in cities in order to get out of poverty. At the same time,in the context of the policy of precise poverty alleviation and rural rejuvenation,various helping and supporting measures for rural areas have also emerged. Supporting education in universities is one of the most important ones. With the Graduate Teaching Fellowship of Huazhong Agricultural University and the students and parents in the teaching sites as research objects,the impact of labor mobility and fixed-position supporting education in rural areas on rural families was explored in this paper using economics,sociology and other relevant theories and analytical methods. The results showed that labor mobility can promote the increase of rural household economic income. Outbound industries and regions have a major impact on the entire family development. However,the outflow of labor can also lead to various problems such as staying behind. The fixed-position supporting education in colleges and universities can,on the one hand,promote the improvement of the academic performance of the students in the teaching areas. On the other hand,it can help students develop good habits. Fixed-position supporting education of colleges and universities can be seen as a way to make up for the outflow of labor in rural areas. Combining the flow of labor force with the teaching support of colleges and universities can better promote the development of the entire family of farmers in rural areas.

以深静脉血栓首诊的腹壁型侵袭性纤维瘤病一例并文献复习

纤维瘤病,也称韧带样纤维瘤病或侵袭性纤维瘤病,是一种罕见的具有局部侵袭特性的软组织肿瘤,起源于筋膜或肌肉组织,好发于腹部、胸壁、肠系膜根部和四肢等部位^([1-3])。患者通常症状轻微或无症状,往往因为肿块增大引起压迫症状时才被发现,误诊率较高。现将中南大学湘雅二医院老年外科收治的1例以下肢深静脉血栓为首诊的腹壁侵袭性纤维瘤病报道如下。