目的:筛选胃癌患者及健康人血清外泌体miRNAs,探究miRNAs联合肿瘤标记物在胃癌诊断中的价值。方法:选取2020年4月至2021年4月在苏州大学附属第一医院确诊胃癌的46例患者为胃癌组,同期20例健康体检者为对照组,收集两组血清标本,行血清外...目的:筛选胃癌患者及健康人血清外泌体miRNAs,探究miRNAs联合肿瘤标记物在胃癌诊断中的价值。方法:选取2020年4月至2021年4月在苏州大学附属第一医院确诊胃癌的46例患者为胃癌组,同期20例健康体检者为对照组,收集两组血清标本,行血清外泌体miRNAs测序,筛选并验证差异性表达的miRNAs,将验证后的miRNAs表达水平、肿瘤标志物数值与胃癌诊断行Spearman相关分析,并绘制受试者工作特征(receiver operating characteristic,ROC)曲线,分别分析外泌体miRNAs、CA72-4及两者联合在胃癌诊断中的价值。结果:两组血清外泌体共筛选出376个差异表达的miRNAs,经q-PCR验证,仅4个miRNAs差异具有统计学意义,采用ROC曲线判断外泌体miRNAs在胃癌诊断中的效能:miR-1323、miR-26a-5p、miR-202-3p、miR-96-5p诊断胃癌的曲线下面积(area under curve,AUC)分别为0.908、0.815、0.570、0.547,灵敏度分别为71.7%、67.4%、30.4%、19.6%,特异度分别100%、85.0%、90.0%、100%。Spearman相关分析结果提示:miR-26a-5p、miR-1323和CA72-4与胃癌诊断相关,ROC曲线显示:miR-26a-5p、miR-1323和CA72-4三者联合诊断的曲线下面积为0.967,高于三者单独诊断效能,其灵敏度和特异度分别为87.0%、100%。结论:联合检测血清外泌体miR-26a-5p、miR-1323和CA72-4的表达水平,在胃癌诊断中具有潜在价值。展开更多
In the treatment of most malignancies,radiotherapy plays a significant role.However,the resistance of cancer cells to ionizing radiation(IR)is the main reason for the failure of radiotherapy,which causes tumor recurre...In the treatment of most malignancies,radiotherapy plays a significant role.However,the resistance of cancer cells to ionizing radiation(IR)is the main reason for the failure of radiotherapy,which causes tumor recurrence and metastasis.In this study,we confirmed that GPR162,an orphan receptor in the G-protein-coupled receptor family,acted as a novel radiotherapy sensitizer by interacting with the stimulator of interferon genes(STING),which targeted DNA damage responses,activated IRF3,accelerated the activation of type I interferon system,promoted the expression of chemokines including CXCL10 and CXCL4,and inhibited the occurrence and development of tumors.Interestingly,the activation of STING by overexpression of GPR162 was independent of the classical pathway of cGAS.STING inhibitors could resist the antitumor effect of overexpression of GPR162 in IR-induced mouse models.In addition,most solid tumors showed low expression of GPR162.And the higher expression of GPR162 indicated a better prognosis in patients with lung adenocarcinoma,liver cancer,breast cancer,etc.In summary,these results suggested that GPR162 may serve as a potential sensitizer of radiotherapy by promoting radiotherapy-induced STING-IFN production and increasing the expression of chemokines including CXCL10 and CXCL4 in DNA damage response,providing an alternative strategy for improving cancer radiotherapy.展开更多
Since the publication of this article,we noticed a minor mistake in the article that needs to be corrected.We have checked the original data;the correct data are provided in this Corrigendum as follows.The key finding...Since the publication of this article,we noticed a minor mistake in the article that needs to be corrected.We have checked the original data;the correct data are provided in this Corrigendum as follows.The key findings of the article are not affected by these corrections.展开更多
文摘目的:筛选胃癌患者及健康人血清外泌体miRNAs,探究miRNAs联合肿瘤标记物在胃癌诊断中的价值。方法:选取2020年4月至2021年4月在苏州大学附属第一医院确诊胃癌的46例患者为胃癌组,同期20例健康体检者为对照组,收集两组血清标本,行血清外泌体miRNAs测序,筛选并验证差异性表达的miRNAs,将验证后的miRNAs表达水平、肿瘤标志物数值与胃癌诊断行Spearman相关分析,并绘制受试者工作特征(receiver operating characteristic,ROC)曲线,分别分析外泌体miRNAs、CA72-4及两者联合在胃癌诊断中的价值。结果:两组血清外泌体共筛选出376个差异表达的miRNAs,经q-PCR验证,仅4个miRNAs差异具有统计学意义,采用ROC曲线判断外泌体miRNAs在胃癌诊断中的效能:miR-1323、miR-26a-5p、miR-202-3p、miR-96-5p诊断胃癌的曲线下面积(area under curve,AUC)分别为0.908、0.815、0.570、0.547,灵敏度分别为71.7%、67.4%、30.4%、19.6%,特异度分别100%、85.0%、90.0%、100%。Spearman相关分析结果提示:miR-26a-5p、miR-1323和CA72-4与胃癌诊断相关,ROC曲线显示:miR-26a-5p、miR-1323和CA72-4三者联合诊断的曲线下面积为0.967,高于三者单独诊断效能,其灵敏度和特异度分别为87.0%、100%。结论:联合检测血清外泌体miR-26a-5p、miR-1323和CA72-4的表达水平,在胃癌诊断中具有潜在价值。
基金The Science and Technology Innovation Program of Hunan Province[2022RC3072(Y.Tao)].
文摘In the treatment of most malignancies,radiotherapy plays a significant role.However,the resistance of cancer cells to ionizing radiation(IR)is the main reason for the failure of radiotherapy,which causes tumor recurrence and metastasis.In this study,we confirmed that GPR162,an orphan receptor in the G-protein-coupled receptor family,acted as a novel radiotherapy sensitizer by interacting with the stimulator of interferon genes(STING),which targeted DNA damage responses,activated IRF3,accelerated the activation of type I interferon system,promoted the expression of chemokines including CXCL10 and CXCL4,and inhibited the occurrence and development of tumors.Interestingly,the activation of STING by overexpression of GPR162 was independent of the classical pathway of cGAS.STING inhibitors could resist the antitumor effect of overexpression of GPR162 in IR-induced mouse models.In addition,most solid tumors showed low expression of GPR162.And the higher expression of GPR162 indicated a better prognosis in patients with lung adenocarcinoma,liver cancer,breast cancer,etc.In summary,these results suggested that GPR162 may serve as a potential sensitizer of radiotherapy by promoting radiotherapy-induced STING-IFN production and increasing the expression of chemokines including CXCL10 and CXCL4 in DNA damage response,providing an alternative strategy for improving cancer radiotherapy.
文摘Since the publication of this article,we noticed a minor mistake in the article that needs to be corrected.We have checked the original data;the correct data are provided in this Corrigendum as follows.The key findings of the article are not affected by these corrections.
