Respirasome, a huge molecular machine that carries out cellular respiration, has gained growing attention since its discovery, because respiration is the most indis- pensable biological process in almost all living cr...Respirasome, a huge molecular machine that carries out cellular respiration, has gained growing attention since its discovery, because respiration is the most indis- pensable biological process in almost all living crea- tures. The concept of respirasome has renewed our understanding of the respiratory chain organization, and most recently, the structure of respirasome solved by Yang＇s group from Tsinghua University （Gu et al. Nature 237（7622）：639-643, 2016） firstly presented the detailed interactions within this huge molecular machine, and provided important information for drug design and screening. However, the study of cellular respiration went through a long history. Here, we briefly showed the detoured history of respiratory chain investigation, and then described the amazing structure of respirasome.展开更多
Respirasome,as a vital part of the oxidative phosphorylation system,undertakes the task of transferring electrons from the electron donors to oxygen and produces a proton concentration gradient across the inner mitoch...Respirasome,as a vital part of the oxidative phosphorylation system,undertakes the task of transferring electrons from the electron donors to oxygen and produces a proton concentration gradient across the inner mitochondrial membrane through the coupled translocation of protons.Copious research has been carried out on this lynchpin of respiration.From the discovery of individual respiratory complexes to the report of the high-resolution structure of mammalian respiratory supercomplex I1III2IV1,scientists have gradually uncovered the mysterious veil of the electron transport chain(ETC).With the discovery of the mammalian respiratory mega complex I2III2IV2,a new perspective emerges in the research field of the ETC.Behind these advances glitters the light of the revolution in both theory and technology.Here,we give a short review about how scientists‘see’the structure and the mechanism of respirasome from the macroscopic scale to the atomic scale during the past decades.展开更多
Dear Editor, Mitochondrial respiratory chain consists of four multimeric protein complexes, Complex I-IV （CI, NADH dehydroge- nase; CII, succinate：ubiquinone oxidoreductase; Clll, cyto- chrome bcl complex; and CIV, ...Dear Editor, Mitochondrial respiratory chain consists of four multimeric protein complexes, Complex I-IV （CI, NADH dehydroge- nase; CII, succinate：ubiquinone oxidoreductase; Clll, cyto- chrome bcl complex; and CIV, cytochrome c oxidase）. These four complexes transfer electrons from NADH or FADH2 to oxygen and pump protons from mitochondrial matrix to intermembrane space, generating electrochemical gradient across the inner membrane which is harnessed by complex V to synthesize ATP, providing the majority of energy acquired by living organisms. Respiratory chain complexes were reported to interact with each other to form supercomplexes.展开更多
基金This work was supported by funds from the National Basic Research Program （973 Program） （Nos. 2016YFA0501101 and 2012CB911101）, and the National Science Fund for Distinguished Young Scholars （No. 3163000168） and the National Natural Science Foundation of China （Grant Nos. 31030020 and 31170679）.
文摘Respirasome, a huge molecular machine that carries out cellular respiration, has gained growing attention since its discovery, because respiration is the most indis- pensable biological process in almost all living crea- tures. The concept of respirasome has renewed our understanding of the respiratory chain organization, and most recently, the structure of respirasome solved by Yang＇s group from Tsinghua University （Gu et al. Nature 237（7622）：639-643, 2016） firstly presented the detailed interactions within this huge molecular machine, and provided important information for drug design and screening. However, the study of cellular respiration went through a long history. Here, we briefly showed the detoured history of respiratory chain investigation, and then described the amazing structure of respirasome.
基金Tsinghua University Branch of China National Center for Protein Sciences(Beijing)for providing the facility support.
文摘Respirasome,as a vital part of the oxidative phosphorylation system,undertakes the task of transferring electrons from the electron donors to oxygen and produces a proton concentration gradient across the inner mitochondrial membrane through the coupled translocation of protons.Copious research has been carried out on this lynchpin of respiration.From the discovery of individual respiratory complexes to the report of the high-resolution structure of mammalian respiratory supercomplex I1III2IV1,scientists have gradually uncovered the mysterious veil of the electron transport chain(ETC).With the discovery of the mammalian respiratory mega complex I2III2IV2,a new perspective emerges in the research field of the ETC.Behind these advances glitters the light of the revolution in both theory and technology.Here,we give a short review about how scientists‘see’the structure and the mechanism of respirasome from the macroscopic scale to the atomic scale during the past decades.
基金We thank the Tsinghua University Branch of China National Center for Protein Sciences （Beijing） for providing the facility support. The computation was completed on the ＂Explorer 100＂ cluster system of Tsinghua National Laboratory for Information Science and Technol- ogy. This work was supported by funds from the National Key R＆D Program of China （Nos. 2017YFA0504600 and 2016YFA0501100）. The National Science Fund for Distinguished Young Scholars（31625008）, and the National Natural Science Foundation of China （Grant Nos. 21532004 and 31570733）.
文摘Dear Editor, Mitochondrial respiratory chain consists of four multimeric protein complexes, Complex I-IV （CI, NADH dehydroge- nase; CII, succinate：ubiquinone oxidoreductase; Clll, cyto- chrome bcl complex; and CIV, cytochrome c oxidase）. These four complexes transfer electrons from NADH or FADH2 to oxygen and pump protons from mitochondrial matrix to intermembrane space, generating electrochemical gradient across the inner membrane which is harnessed by complex V to synthesize ATP, providing the majority of energy acquired by living organisms. Respiratory chain complexes were reported to interact with each other to form supercomplexes.
