[Objectives] To compare the differences in the total flavonoids between leaves and whole plants of Anoectochilus roxburghii.[Methods] The total flavonoids in the tissue cultured leaves and whole plants of A.roxburghii...[Objectives] To compare the differences in the total flavonoids between leaves and whole plants of Anoectochilus roxburghii.[Methods] The total flavonoids in the tissue cultured leaves and whole plants of A.roxburghii were extracted by microwave-assisted extraction.The content of total flavonoids in the extracts was determined by UV spectrophotometry.[Results]The results of the content determination showed that there were significant differences in the flavonoid content between the leaves and the whole plants of A.roxburghii.[Conclusions]The content of total flavonoids in tissue cultured leaves was higher than that of whole plants.Thus,in the development and utilization of A.roxburghii,tissue cultured leaves can be used to take the place of the whole plants.展开更多
基金Supported by the Forestry Science and Technology Extension Project of Central Finance "Tissue Culture Rapid Propagation and Cultivation Techniques for Anoectochilus roxburghii"(JXTG[2016]-05)
文摘[Objectives] To compare the differences in the total flavonoids between leaves and whole plants of Anoectochilus roxburghii.[Methods] The total flavonoids in the tissue cultured leaves and whole plants of A.roxburghii were extracted by microwave-assisted extraction.The content of total flavonoids in the extracts was determined by UV spectrophotometry.[Results]The results of the content determination showed that there were significant differences in the flavonoid content between the leaves and the whole plants of A.roxburghii.[Conclusions]The content of total flavonoids in tissue cultured leaves was higher than that of whole plants.Thus,in the development and utilization of A.roxburghii,tissue cultured leaves can be used to take the place of the whole plants.
