In plants,alternative splicing(AS)is markedly induced in response to environmental stresses,but it is unclear why plants generate multiple transcripts under stress conditions.In this study,RNA-seq was performed to ide...In plants,alternative splicing(AS)is markedly induced in response to environmental stresses,but it is unclear why plants generate multiple transcripts under stress conditions.In this study,RNA-seq was performed to identify AS events in cucumber seedlings grown under different light intensities.We identi fi ed a novel transcript of the gibberellin(GA)-deactivating enzyme Gibberellin 2-beta-dioxygenase 8(CsGA2ox8).Compared with canonical CsGA2ox8.1,the CsGA2ox8.2 isoform presented intron retention between the second and third exons.Functional analysis proved that the transcript of CsGA2ox8.1 but not CsGA2ox8.2 played a role in the deactivation of bioactive GAs.Moreover,expression analysis demonstrated that both transcripts were upregulated by increased light intensity,but the expression level of CsGA2ox8.1 increased slowly when the light intensity was>400μmol-m-2-s-1 PPFD(photosynthetic photon fl ux density),while the CsGA2ox8.2 transcript levels increased rapidly when the light intensity was>200 pmol-m-2-s-1 PPFD.Our fi ndings provide evidence that plants might fi nely tune their GA levels by buffering against the normal transcripts of CsGA2ox8 through AS.展开更多
基金The Nati onal Key Resea rch and Developme nt Program of China(2019YFD1000300)the International Postdoctoral Exchange Fellowship Program from the China Postdoctoral Council(20170053)+1 种基金the Tech no logy System Constr ucti on of Mode rn Agr icult ural In dust ry of Sha nghai(19Z113040008)the Presidential Foundation of Guangdong Academy of Agricultural Sciences(BZ201901).
文摘In plants,alternative splicing(AS)is markedly induced in response to environmental stresses,but it is unclear why plants generate multiple transcripts under stress conditions.In this study,RNA-seq was performed to identify AS events in cucumber seedlings grown under different light intensities.We identi fi ed a novel transcript of the gibberellin(GA)-deactivating enzyme Gibberellin 2-beta-dioxygenase 8(CsGA2ox8).Compared with canonical CsGA2ox8.1,the CsGA2ox8.2 isoform presented intron retention between the second and third exons.Functional analysis proved that the transcript of CsGA2ox8.1 but not CsGA2ox8.2 played a role in the deactivation of bioactive GAs.Moreover,expression analysis demonstrated that both transcripts were upregulated by increased light intensity,but the expression level of CsGA2ox8.1 increased slowly when the light intensity was>400μmol-m-2-s-1 PPFD(photosynthetic photon fl ux density),while the CsGA2ox8.2 transcript levels increased rapidly when the light intensity was>200 pmol-m-2-s-1 PPFD.Our fi ndings provide evidence that plants might fi nely tune their GA levels by buffering against the normal transcripts of CsGA2ox8 through AS.
