Knockdown of RFC4 inhibits the cell proliferation of nasopharyngeal carcinoma in vitro and in vivo

Nasopharyngeal carcinoma(NPC)is a malignant tumor that mainly occurs in East and Southeast Asia.Although patients benefit from the main NPC treatments(e.g.,radiotherapy and concurrent chemotherapy),persistent and recurrent diseases still occur in some NPC patients.Therefore,investigating the pathogenesis of NPC is of great clinical significance.In the present study,replication factor c subunit 4(RFC4)is a key potential target involved in NPC progression via bioinformatics analysis.Furthermore,the expression and mechanism of RFC4 in NPC were investigated in vitro and in vivo.Our results revealed that RFC4 was more elevated in NPC tumor tissues than in normal tissues.RFC4 knockdown induced G2/M cell cycle arrest and inhibited NPC cell proliferation in vitro and in vivo.Interestingly,HOXA10 was confirmed as a downstream target of RFC4,and the overexpression of HOXA10 attenuated the silencing of RFC4-induced cell proliferation,colony formation inhibition,and cell cycle arrest.For the first time,this study reveals that RFC4 is required for NPC cell proliferation and may play a pivotal role in NPC tumorigenesis.

会计信息可比性的测度方法研究——基于函数型数据分析的改进

测度会计信息可比性的方法主要包括会计准则趋同检验、会计方法可比性测度及基于公司层面的会计信息可比性测度等三类,其中,前两类方法只是会计信息可比性测度的必要非充分条件,而目前基于公司层面的会计信息可比性测度的线性框架(单变量盈利—收益一次方程)不仅难以捕捉会计盈余与股票收益率之间可能隐藏的高阶相关关系,而且忽略了公司层面的微观差异和动态结构,本文应用函数型数据分析模型在这两个方面尝试做出改进。实证研究发现:会计盈余与股票收益率之间的弱相关关系只是表达了变量之间的线性结构,二者之间的非线性关系其实并不弱,平均会计盈余对平均股票收益率存在显著的响应与伴随关系,且这种关系是动态的。因此进一步应用函数型数据分析模型构建基于公司层面的会计可比性测度,经检验,该方法具有较强的稳健性和有效性,拟合效果更好,并且通过函数曲线不同阶数的导函数可以刻画出公司层面的微观差异和动态结构。该方法为探索适合我国公司的会计信息可比性测度方法提供了理论参考,也为提高会计信息质量提供了决策依据。