基于网络药理学探讨罗勒抗炎抗菌分子机制

利用网络药理学探究罗勒抗炎抗菌的作用机制,并通过体外试验进行初步验证。通过相关数据库筛选到罗勒的18种化合物和6种挥发油成分,作用靶点521个,获得抗炎抗菌相关靶点975个。使用Venny 2.1做药物和疾病靶点交集的韦恩图,得到88个罗勒抗炎抗菌靶点。构建蛋白互作PPI网络,并进行GO和KEGG富集分析。根据蛋白关联度可知核心靶点涉及TNF-α,IL-1β,IL-6等,主要通过对细菌的反应、对脂多糖的反应等信号通路发挥抗炎抗菌作用。体外细胞试验采用LPS及热灭活痤疮丙酸杆菌(Cutibacterium acnes,C.a)诱导的THP-1两种炎症细胞模型。采用ELISA法检测添加不同含量罗勒精油(Ocimum basilicum L.essential oil,OBEO)细胞的TNF-α,IL-1β、IL-6的分泌量。结果显示,体积分数为0.025%的OBEO能够抑制两种炎症损伤模型TNF-α,IL-1β,IL-6的表达;且OBEO对灭活C.a致THP-1细胞炎症损伤的抑制作用显著,分别降低TNF-α,IL-1β,IL-6的表达24%,45%,13%左右。

网络药理学研究现状及在化妆品领域应用展望

网络药理学的出现是当前人工智能和大数据时代发展的结果,对医药领域疾病的治疗和新药的研发起着十分重要的作用,但未有关于网络药理学在化妆品领域中的相关应用研究。本文从药理学及中医药研究、复杂性疾病揭示、药物创制等几个方面回顾和梳理了网络药理学应用研究的发展现状,并对未来网络药理学在化妆品植物功效原料的选择、化妆品功效性评价、化妆品安全性评价三方面提出了应用设想。

Research Progress of Bioactive Substances and Processing of Abelmoschus esculentus L.

Okra （Abelmoschus esculentus L. ） is a novel heahhcare vegetable that has been developed rapidly in recent years in China. It contains abundant bioactive substances with significant heahbeare functions. So far, many domestic research institutions have carried out researches about the extraction technology of bioactive substances and their heahhcare functions. At present, tender pods and other organs of A. esculentus are mainly used freshly and directly, but rare products have been processed and developed. In this paper, the research progress of bioactive substances and processing ofA. esculentus was summarized, aiming at providing reference for the deep processing and comprehensive utilization of A. escu/entus in the future in China.