Cotton fiber is the most important natural raw material for the textile industry, and fiber length(FL) is one of the most important traits in cotton.Quantitative trait locus(QTL)mapping based on high-density genetic m...Cotton fiber is the most important natural raw material for the textile industry, and fiber length(FL) is one of the most important traits in cotton.Quantitative trait locus(QTL)mapping based on high-density genetic maps is an efficient approach to identify genetic regions for FL.In our study, two backcrossed inbred lines(BILs) were chosen as parents to construct a high-density genetic map in F2 which was used to fine map FL QTL in F2:3 population.The genetic map had a total size of 3462.8 cM, containing 9182 singlenucleotide polymorphisms(SNPs) based on genotyping-by-sequencing.Two FL related stable QTL were identified on two chromosomes(qFL-A08–1 on A08 and qFL-D03–1 on D03),and qFL-A08–1 was confirmed by a meta-analysis.Utilizing previously obtained RNA-seq data for the two BILs and qRT-PCR analysis, two candidate genes annotated as cytochrome b5(CB5, Gh_A08 G1729) and microtubule end-binding 1 C(EB1C, Gh_D03 G0232) that may regulate FL during the fiber elongation stage were identified.In addition, nine recombination hotspots in this population were found.The results of this study will provide an important foundation for further studies on the molecular and genetic regulation of fiber elongation.展开更多
The cotton fiber is the most important raw material for the textile industry and an ideal model system for studying cell elongation. However, the genetic variation of fiber elongation in relation to miRNA is poorly un...The cotton fiber is the most important raw material for the textile industry and an ideal model system for studying cell elongation. However, the genetic variation of fiber elongation in relation to miRNA is poorly understood. A high-throughput comparative RNA-seq of two lines differing in fiber length(FL) from a backcross inbred line(BIL)population of G. hirsutum × G. barbadense revealed differentially expressed(DE) miRNAs and their targets in rapidly elongating fibers. A real-time quantitative PCR analysis was further performed to validate the results. A total of 463(including 47 DE) miRNAs were identified,and seven DE miRNAs were co-localized with seven FL quantitative trait loci(QTL)identified in the G. hirsutum × G. barbadense population. Of 82(including 21 DE) targets identified, nine(including one DE) were also co-localized with the seven FL QTL. The relationship between the allopolyploid and its diploid ancestral species with respect to miRNAs and their targets was also characterized. These results will facilitate the understanding of the molecular genetic mechanism of fiber elongation with regards to miRNAs in cotton.展开更多
A high-density linkage map was constructed for an F2 population derived from an Interspecific cross of cultivated allotetraploid species between Gossypium hirsutum L. and G. barbadense L. A total of 186 F2 individuals...A high-density linkage map was constructed for an F2 population derived from an Interspecific cross of cultivated allotetraploid species between Gossypium hirsutum L. and G. barbadense L. A total of 186 F2 individuals from the Interspecific cross of ＂CRI 36 × Hal 7124＂ were genotyped at I 252 polymorphic loci Including a novel marker system, target region amplification polymorphism （TRAP）. The map consists of 1 097 markers, including 697 simple se- quence repeats （SSRs）, 171 TRAPs, 129 sequence-related amplified polymorphisms, 98 amplified fragment length polymorphisms, and two morphological markers, and spanned 4 536.7 cM with an average genetic distance of 4.1 cM per marker. Using 45 duplicated SSR loci among chromosomes, 11 of the 13 pairs of homologous chromosomes were Identified In tetraploid cotton. This map will provide an essential resource for high resolution mapping of quantitative trait loci and molecular breeding in cotton.展开更多
The self-dissipation and attenuation capacity of materials play an important role in realizing efficient electromagnetic absorption,in this case,the roles of macroscopic composition and micro-structure should be empha...The self-dissipation and attenuation capacity of materials play an important role in realizing efficient electromagnetic absorption,in this case,the roles of macroscopic composition and micro-structure should be emphasized simultaneously in the reasonable design of microwave absorbent.Given that,Fe_(3)N alloy embedded in two-dimensional porous carbon composites were fabricated via facile sol-gel and sacrificial template methods.Satisfactorily,the magnetic/dielectric materials combination and porous structure introduction are conductive to the optimization of impedance matching property,as result of the enhancement of microwave absorption capacity.In addition,sufficient magnetic loss capacity,strong conductivity as well as polarization attenuation bring about the outstanding microwave absorbing performance with an effective absorption bandwidth of 6.76 GHz and a minimum reflection loss value of-65.6 d B.It is believed that this work not only lay a foundation to achieve microwave response materials in a wide frequency range,but also emphasize the significant role of the component selection and structural design.展开更多
基金supported by the National Natural Science Foundation of China (31621005)the National Key Research and Development Program of China (2016YFD0101400)+1 种基金the National Transgenic Research Program of China (2016ZX08005005)the New Mexico Agricultural Experiment Station
文摘Cotton fiber is the most important natural raw material for the textile industry, and fiber length(FL) is one of the most important traits in cotton.Quantitative trait locus(QTL)mapping based on high-density genetic maps is an efficient approach to identify genetic regions for FL.In our study, two backcrossed inbred lines(BILs) were chosen as parents to construct a high-density genetic map in F2 which was used to fine map FL QTL in F2:3 population.The genetic map had a total size of 3462.8 cM, containing 9182 singlenucleotide polymorphisms(SNPs) based on genotyping-by-sequencing.Two FL related stable QTL were identified on two chromosomes(qFL-A08–1 on A08 and qFL-D03–1 on D03),and qFL-A08–1 was confirmed by a meta-analysis.Utilizing previously obtained RNA-seq data for the two BILs and qRT-PCR analysis, two candidate genes annotated as cytochrome b5(CB5, Gh_A08 G1729) and microtubule end-binding 1 C(EB1C, Gh_D03 G0232) that may regulate FL during the fiber elongation stage were identified.In addition, nine recombination hotspots in this population were found.The results of this study will provide an important foundation for further studies on the molecular and genetic regulation of fiber elongation.
基金supported by grants from the National Natural Science Foundation of China(31621005)the National Key Research and Development Program of China(2016YFD0101400)+1 种基金the National Research and Development Project of Transgenic Crops of China(2016ZX08005005)the New Mexico Agricultural Experiment Station
文摘The cotton fiber is the most important raw material for the textile industry and an ideal model system for studying cell elongation. However, the genetic variation of fiber elongation in relation to miRNA is poorly understood. A high-throughput comparative RNA-seq of two lines differing in fiber length(FL) from a backcross inbred line(BIL)population of G. hirsutum × G. barbadense revealed differentially expressed(DE) miRNAs and their targets in rapidly elongating fibers. A real-time quantitative PCR analysis was further performed to validate the results. A total of 463(including 47 DE) miRNAs were identified,and seven DE miRNAs were co-localized with seven FL quantitative trait loci(QTL)identified in the G. hirsutum × G. barbadense population. Of 82(including 21 DE) targets identified, nine(including one DE) were also co-localized with the seven FL QTL. The relationship between the allopolyploid and its diploid ancestral species with respect to miRNAs and their targets was also characterized. These results will facilitate the understanding of the molecular genetic mechanism of fiber elongation with regards to miRNAs in cotton.
文摘A high-density linkage map was constructed for an F2 population derived from an Interspecific cross of cultivated allotetraploid species between Gossypium hirsutum L. and G. barbadense L. A total of 186 F2 individuals from the Interspecific cross of ＂CRI 36 × Hal 7124＂ were genotyped at I 252 polymorphic loci Including a novel marker system, target region amplification polymorphism （TRAP）. The map consists of 1 097 markers, including 697 simple se- quence repeats （SSRs）, 171 TRAPs, 129 sequence-related amplified polymorphisms, 98 amplified fragment length polymorphisms, and two morphological markers, and spanned 4 536.7 cM with an average genetic distance of 4.1 cM per marker. Using 45 duplicated SSR loci among chromosomes, 11 of the 13 pairs of homologous chromosomes were Identified In tetraploid cotton. This map will provide an essential resource for high resolution mapping of quantitative trait loci and molecular breeding in cotton.
基金supported by the National Nature Science Foundation of China(51971111)the Postgraduate Research&Practice Innovation of Jiangsu Province(KYCX200190)Jiangsu Provincial Key Laboratory of Bionic Functional Materials。
文摘为了满足电子通信产品对电磁兼容不断增长的需求,研制轻质、柔性、高效电磁干扰(EMI)屏蔽材料已成为研究热点之一.本文以聚(3,4-乙撑二氧噻吩):聚(苯乙烯磺酸盐)(PEDOT:PSS)和银纳米线(Ag NWs)作为导电填料,通过简便的蒸发法构筑了具有优异机械性能的EMI屏蔽复合膜.通过在PVA/PEDOT:PSS复合材料中添加1.38 wt%Ag NWs,所得薄膜展现出卓越的EMI屏蔽性能,10 GHz,20μm厚度下可达到16,800 dB cm^(-1).此外,PVA/PEDOT:PSS/Ag NWs膜还具有出色的拉伸强度(~62.39 MPa)和断裂伸长率(~29.3%),可满足恶劣环境下的使用需求.综上,该研究为设计新型柔性电磁屏蔽复合薄膜指明了研究方向.
基金supported by the National Natural Science Foundation of China(No.51971111)the Postgraduate Research&Practice Innovation of Jiangsu Province(No.KYCX200190)the Jiangsu Provincial Key Laboratory of Bionic Functional Materials。
文摘The self-dissipation and attenuation capacity of materials play an important role in realizing efficient electromagnetic absorption,in this case,the roles of macroscopic composition and micro-structure should be emphasized simultaneously in the reasonable design of microwave absorbent.Given that,Fe_(3)N alloy embedded in two-dimensional porous carbon composites were fabricated via facile sol-gel and sacrificial template methods.Satisfactorily,the magnetic/dielectric materials combination and porous structure introduction are conductive to the optimization of impedance matching property,as result of the enhancement of microwave absorption capacity.In addition,sufficient magnetic loss capacity,strong conductivity as well as polarization attenuation bring about the outstanding microwave absorbing performance with an effective absorption bandwidth of 6.76 GHz and a minimum reflection loss value of-65.6 d B.It is believed that this work not only lay a foundation to achieve microwave response materials in a wide frequency range,but also emphasize the significant role of the component selection and structural design.
