Effects of Diet with Picria fel-tarrae Lour Particle on the Growth Performance, Blood Biochemical Indexes, Immune and Antioxidant Function of Hongguang Yellow Chickens

[Objectives]To investigate the effect of diet with Picria fel-tarrae Lour particle(PFLp)on growth performance,blood biochemical indexes,immune and antioxidant function of Hongguang yellow chickens(HYC).[Methods]80 HYC in age of 1 d were divided into 4 groups randomly and each group was assigned to 5 replicates with 24 chicks in each replicate.Group A was control group fed only basal diet.Group B,C and D were treatment groups and fed diets with 0.125%,0.25%and 0.50%of PFLp respectively during the trial of 49 d.Serum samples were taken from 10 chicks in each group in the age of 21,35 and 49 d respectively and analyzed for biochemical,immune and antioxidant parameters in blood.[Results]ADG from group C in 21 d was increased significantly than that from group A,B and D(P<0.01),and ADG in 35 d from group C was also significantly greater than that from group A,B and D(P<0.05),but not much difference was found on ADG among the 4 groups in 49 d(P>0.05);ADFI from group C in 35 d was significantly higher than that from group A,B and D(P<0.05).ADFI from group C in 21 and 49 d were also higher than that from group A(P>0.05).FCR from group C in 21 d was much better than that from the other three groups(P<0.01).Death rate of group C was 50 percent lower than group A;There was no difference occurred in serum biochemical and immune parameters among the 4 groups during the trial(P>0.05).However,the T-AOC from group C and D in 21 d was enhanced significantly than that from group A and B(P<0.01),and the one from group C in 35 d was also increased greatly than that from group A,B and D(P<0.05).GSH from group C and D in 21 d was much higher than that from group A and B(P<0.05),and the ones from group C and D in 35 d were also significantly higher than those from group A and B(P<0.01).T-SOD in three stages trended up also in group C and D(P>0.05).[Conclusions]The diet with 0.25%PFLp in 21 and 35 d could dramatically improve the growth performance,significantly increase the antioxidant capacity and effectively reduce death rate.Diet with 0.25%PFLp is the most appropriate among the three different additions.

Effect of monoclonal antibody to bull sperm sp18 on murine fertilization and embryos development

Western blot analysis revealed that one IgG1 monoclonal antibody （mAb） to sp18 family membrane proteins （Mr. 14, 16 and 18 ku） of bovine sperm reacted faintly with protein bands of 14,18, 22, 30 and 60 ku （reducing） in samples of mouse sperm. The mAb also reacted to protein of egg lysozyme. Using a laser confocal microscope, indirect immunofluorescence （IIF） showed that the sp18 antigens were present in the posterior head of murine sperm. In murine in vitro fertilization （IVF） and embryo development trails, a total of 426 oocytes from C57BL/6 and F1 hybrid strain (CD1×C57BL/6 cross) of 12, female mice were used in 3 independent trails. After preincubating capacitated sperm with 182 μg/mL of sp18 mAb in the modified TYH IVF medium for 15—20 min, cumulus-oocyte-complexes were introduced. The fertilization rate in sp18 mAb groups was 77.1%, which was not significantly （P】0.05） different from the nonspecific mouse IgG（79.2 %） and non-IgG（80.3%） control groups. Fertilized oocytes had been

Correction of RNA aberrant splice increases foreign gene expression in transgenic mice

Transgenic mice with mammary gland secreting human granulocyte colony stimulating factor (G-CSF) were produced using mice whey acid protein gene promoter. It was found that there was very low expression level in mammary gland. Human G-CSF cDNA was obtained by RT-PCR from transgenic mice mammary gland. Sequence analysis showed that this G-CSF gene deleted the 4th exon, and compared with human G-CSF genomic DNA, there were donor and acceptor splice sites in the deletion fragment. It was considered that the 3rd and 4th introns also delete in G-CSF fragment. The transgenic construct was corrected by deleting the 3rd and 4th introns to construct the minigene, which was used to produce transgenic mice by microinjection. Northern blot showed that G-CSF expression using the new construct increased 5.4 times as that before in transgenic mice. The results suggested that it was possible that RNA aberrant splice result in low expression in transgenic mice.

Nuclear transfer of goat somatic cells transgenic for human lactoferrin gene

Transgenic animal mammary gland bioreactors are used to produce recombinant proteins with appropriate post-translational modifications.The nuclear transfer of transgenic somatic cells is a powerful method to produce mammary gland bioreactors.We established an efficient gene transfer and nuclear transfer approach in goat somatic cells.Gene targeting vector pGBC2LF was constructed by cloning human lactoferrin(LF)gene cDNA into exon 2 of the milk goat beta-casein gene and the endogenous start codon was replaced by that of human LF gene.Goat fetal fibroblasts were transfected with lin-earized pGBC2LF and 14 cell lines were positive accord-ing to PCR and Southern blot.The transgenic cells were used as donor cells of nuclear transfer and some of recon-structed embryos could develop into blastocyst in vitro.