Mitochondrial DNA（mt DNA） variation has been implicated in many common complex diseases, but inconsistent and contradicting results are common. Here we introduce a novel mutational load hypothesis, which also consid...Mitochondrial DNA（mt DNA） variation has been implicated in many common complex diseases, but inconsistent and contradicting results are common. Here we introduce a novel mutational load hypothesis, which also considers the collective effect of mainly rare variants, utilising the Mut Pred Program.We apply this new methodology to investigate the possible role of mt DNA in two cardiovascular disease（CVD） phenotypes（hypertension and hyperglycaemia）, within a two-population cohort（n = 363; mean age 45 ± 9 yrs）. Very few studies have looked at African mt DNA variation in the context of complex disease, and none using complete sequence data in a well-phenotyped cohort. As such, our study will also extend our knowledge of African mt DNA variation, with complete sequences of Southern Africans being especially under-represented. The cohort showed prevalence rates for hypertension（58.6%） and prediabetes（44.8%）. We could not identify a statistically significant role for mt DNA variation in association with hypertension or hyperglycaemia in our cohort. However, we are of the opinion that the method described will find wide application in the field, being especially useful for cohorts from multiple locations or with a variety of mt DNA lineages, where the traditional haplogroup association method has been particularly likely to generate spurious results in the context of association with common complex disease.展开更多
During the last decade, hundreds of studies have been pub- lished examining whether significant associations exist be- tween mitochondrial DNA （mtDNA） variants and/or haplogroups （clades） and particular diseases ...During the last decade, hundreds of studies have been pub- lished examining whether significant associations exist be- tween mitochondrial DNA （mtDNA） variants and/or haplogroups （clades） and particular diseases （generally com- mon/complex diseases） （Fig. 1）. However, several authors have gathered evidence indicating a high incidence of false positive findings in mtDNA case-control association studies. Raule et al. （2007） and Herrnstadt and Howell （2004） showed various problems affecting mtDNA case-control association studies. Salas et al.展开更多
基金the Faculty of Natural Sciences of the NorthWest University for contributing to funding and Thermo Fisher South Africa for providing additional technical resources to this studypartially funded by the South African National Research Foundation+4 种基金Medical Research CouncilROCHE DiagnosticsNorth-West University,South Africaas well as the Metabolic Syndrome Institute,Francefunding support from the Royal Society and the National Research Foundation of South Africa, for the academic meeting at which the project was mapped out
文摘Mitochondrial DNA（mt DNA） variation has been implicated in many common complex diseases, but inconsistent and contradicting results are common. Here we introduce a novel mutational load hypothesis, which also considers the collective effect of mainly rare variants, utilising the Mut Pred Program.We apply this new methodology to investigate the possible role of mt DNA in two cardiovascular disease（CVD） phenotypes（hypertension and hyperglycaemia）, within a two-population cohort（n = 363; mean age 45 ± 9 yrs）. Very few studies have looked at African mt DNA variation in the context of complex disease, and none using complete sequence data in a well-phenotyped cohort. As such, our study will also extend our knowledge of African mt DNA variation, with complete sequences of Southern Africans being especially under-represented. The cohort showed prevalence rates for hypertension（58.6%） and prediabetes（44.8%）. We could not identify a statistically significant role for mt DNA variation in association with hypertension or hyperglycaemia in our cohort. However, we are of the opinion that the method described will find wide application in the field, being especially useful for cohorts from multiple locations or with a variety of mt DNA lineages, where the traditional haplogroup association method has been particularly likely to generate spurious results in the context of association with common complex disease.
基金the "Ministerio de Ciencia e Innovacio'n"(No.SAF2011-26983)the Plan Galego IDT(No.EM 2012/045)the grant from the Sistema Universitario Gallego-Modalidad REDES(No.2012-PG226,to A.Salas) from the Xunta de Galicia
文摘During the last decade, hundreds of studies have been pub- lished examining whether significant associations exist be- tween mitochondrial DNA （mtDNA） variants and/or haplogroups （clades） and particular diseases （generally com- mon/complex diseases） （Fig. 1）. However, several authors have gathered evidence indicating a high incidence of false positive findings in mtDNA case-control association studies. Raule et al. （2007） and Herrnstadt and Howell （2004） showed various problems affecting mtDNA case-control association studies. Salas et al.
